Neurochemical Research

, Volume 29, Issue 6, pp 1093–1103

Mining Microarrays for Metabolic Meaning: Nutritional Regulation of Hypothalamic Gene Expression

  • Charles V. Mobbs
  • Kelvin Yen
  • Jason Mastaitis
  • Ha Nguyen
  • Elizabeth Watson
  • Elisa Wurmbach
  • Stuart C. Sealfon
  • Andrew Brooks
  • Stephen R. J. Salton
Article

Abstract

DNA microarray analysis has been used to investigate relative changes in the level of gene expression in the CNS, including changes that are associated with disease, injury, psychiatric disorders, drug exposure or withdrawal, and memory formation. We have used oligonucleotide microarrays to identify hypothalamic genes that respond to nutritional manipulation. In addition to commonly used microarray analysis based on criteria such as fold-regulation, we have also found that simply carrying out multiple t tests then sorting by P value constitutes a highly reliable method to detect true regulation, as assessed by real-time polymerase chain reaction (PCR), even for relatively low abundance genes or relatively low magnitude of regulation. Such analyses directly suggested novel mechanisms that mediate effects of nutritional state on neuroendocrine function and are being used to identify regulated gene products that may elucidate the metabolic pathology of obese ob/ob, lean Vgf-/Vgf-, and other models with profound metabolic impairments.

Brain diabetes fasting hypothalamus microarray obesity 

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Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Charles V. Mobbs
    • 2
    • 3
  • Kelvin Yen
    • 1
    • 2
    • 5
  • Jason Mastaitis
    • 1
    • 2
    • 5
  • Ha Nguyen
    • 1
    • 2
    • 5
  • Elizabeth Watson
    • 1
    • 5
  • Elisa Wurmbach
    • 4
  • Stuart C. Sealfon
    • 1
    • 4
  • Andrew Brooks
    • 6
  • Stephen R. J. Salton
    • 1
    • 3
  1. 1.Fishberg Research Center for NeurobiologyMount Sinai School of MedicineNew York
  2. 2.Kastor Neurobiology of Aging LaboratoriesMount Sinai School of MedicineNew York
  3. 3.Department of GeriatricsMount Sinai School of MedicneNew York
  4. 4.Department of NeurologyMount Sinai School of MedicineNew York
  5. 5.Graduate Training Program in NeuroscienceMount Sinai School of MedicineNew York
  6. 6.Department of Environmental Medicine, Center for Functional GenomicsUniversity of Rochester Medical CenterRochester

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