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Molecular Biology Reports

, Volume 31, Issue 3, pp 197–202 | Cite as

Molecular cloning and characterization of a novel human protein phosphatase 2C cDNA (PP2C ε)

  • Feng Jin
  • Chaoneng Ji
  • Lingfeng Liu
  • Jianfeng Dai
  • Shaohua Gu
  • Xianfei Sun
  • Yi Xie
  • Yumin Mao
Article

Abstract

We have isolated a novel cDNA from the human fetal brain cDNA library with homology to the Mg2+-dependent serine/threonine protein phosphatase 2C (PP2C) family. The cDNA is 3055 bp in length, and the predicted coding region encodes a 360-amino-acid protein, which shows 99% identity to the PP2C ε from rat and mouse. Then we term it human PP2C ε gene. The gene is mapped to chromosome 3q26.1 and contains 4 exons. RT-PCR analysis shows that the PP2C ε is widely expressed in human tissues and the expression levels in heart, placenta, lung, liver, kidney, and pancreas are relatively high.

Abbreviations: PP2Cε– Protein Phospatase 2C ε; RT-PCR – reverse transcriptionalPCR; SAPKs – stress-activated protein kinases; MAPK – mitogen--activated protein kinase.

expression pattern PP2C ε 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Feng Jin
    • 1
  • Chaoneng Ji
    • 1
  • Lingfeng Liu
    • 1
  • Jianfeng Dai
    • 1
  • Shaohua Gu
    • 1
  • Xianfei Sun
    • 1
  • Yi Xie
    • 1
  • Yumin Mao
    • 1
  1. 1.State Key Laboratory of Genetic Engineering, Institute of Genetics, School of life SciencesFudan UniversityShanghaiPeople's Republic of China

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