Molecular Diversity

, Volume 8, Issue 3, pp 177–187 | Cite as

High density peptide microarrays. In situ synthesis and applications

  • Xiaolian Gao
  • Jean Philippe Pellois
  • Younghwa Na
  • Younkee Kim
  • Erdogan Gulari
  • Xiaochuan Zhou


The technologies enabling the creation of large scale, miniaturized peptide or protein microarrays are emerging. The focuses of this review are the synthesis and applications of peptide and peptidomimetic microarrays, especially the light directed parallel synthesis of individually addressable high density peptide microarrays using a novel photogenerated reagent chemistry and digital photolithography (Gao et al., 1998, J. Am. Chem. Soc. 120, 12698; Pellois et al. 2002, Nat. Biotechnol. 20, 922). Concepts related to the synthesis are discussed, such as the reactions of photogenerated acids in the deprotection step of peptide synthesis or oligonucleotide synthesis, and the applications of high density peptide chips in antibody binding assays are discussed. Peptide chips provide versatile tools for probing antigen-antibody, protein-protein, peptide-ligand interactions and are basic components for miniaturization, automation, and system integration in research and clinical diagnosis applications.

antibody binding digital photolithography epitope screening miniaturization parallel synthesis peptide chip peptide microarray PGA PGR chemistry protein binding assay 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Xiaolian Gao
    • 1
  • Jean Philippe Pellois
    • 1
  • Younghwa Na
    • 1
  • Younkee Kim
    • 2
  • Erdogan Gulari
    • 3
  • Xiaochuan Zhou
    • 4
  1. 1.Department of ChemistryUniversity of HoustonHouston
  2. 2.Xeotron CoHouston
  3. 3.Department of Chemical EngineeringUniversity of MichiganAnn Arbor
  4. 4.Atactic Technologies Inc.Houston

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