Microbiology

, Volume 73, Issue 2, pp 176–183 | Cite as

Three New Species of Brevibacteria, Brevibacterium antiquum sp. nov., Brevibacterium aurantiacum sp. nov., and Brevibacterium permense sp. nov.

  • E. Yu. Gavrish
  • V. I. Krauzova
  • N. V. Potekhina
  • S. G. Karasev
  • E. G. Plotnikova
  • O. V. Altyntseva
  • L. A. Korosteleva
  • L. I. Evtushenko
Article

Abstract

This work deals with the taxonomic study of orange-pigmented bacteria isolated from permafrost sediments, rice plots, and soils contaminated with wastes from the chemical and salt industries that were assigned to the genus Brevibacterium on the basis of phenotypic characteristics, as well as of some strains described previously as Brevibacterium linens. The study revealed three genomic species, whose members and the type strains of the closest species of Brevibacterium had DNA similarity levels between 24 and 59%. The strains of the genomic species differed from each other and from the known species of Brevibacterium in some physiological and biochemical characteristics, as well as in the sugar and polyol composition of their teichoic acids. The 16S rDNA sequence analysis confirmed the assignment of the environmental isolates to the genus Brevibacterium and showed the phylogenetic distinction of the three genomic species. The results obtained in this study allow three new Brevibacterium species to be described: Brevibacterium antiquum (type strain VKM Ac-2118T = UCM Ac-411T), Brevibacterium aurantiacum (type strain VKM Ac-2111T = NCDO 739T = ATCC 9175T), and Brevibacterium permense (type strain VKM Ac-2280T = UCM Ac-413T).

actinomycetes Brevibacterium teichoic acids 16S rDNA 

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REFERENCES

  1. 1.
    Collins, M.D., Genus Brevibacterium, The Prokaryotes, Balows, A. et al., Eds., New York: Springer, 1992, vol. 2, pp. 1351–1354.Google Scholar
  2. 2.
    Collins, M.D., Jones, D., Keddie, R.M., and Sneath, P.H.A., Reclassification of Chromobacterium iodinum (Davis) in a Redefined Genus Brevibacterium (Breed) as Brevibacterium iodinum nom. rev., comb. nov., J. Gen. Microbiol., 1980, vol. 120, pp. 1–10.Google Scholar
  3. 3.
    Collins, M.D., Farrow, J.A.E., Goodfellow, M., and Minnikin, D.E., Brevibacterium casei sp. nov. and Brevibacterium epidermidis sp. nov., Syst. Appl. Microbiol., 1983, vol. 4, pp. 388–395.Google Scholar
  4. 4.
    Anderton, W.J. and Wilkinson, S.G., Evidence for the Presence of a New Class of Teichoic Acids in the Cell Wall of Bacterium NCTC9742, J. Gen. Microbiol., 1980, vol. 118, pp. 343–351.Google Scholar
  5. 5.
    Fiedler, F., Schaffler, M.J., and Stackebrandt, E., Biochemical and Nucleic Acid Hybridization Studies on Brevibacterium linens and Related Strains, Arch. Microbiol., 1981, vol. 129, pp. 85–93.Google Scholar
  6. 6.
    Fiedler, F. and Bude, A., Occurrence and Chemistry of Cell Wall Teichoic Acids in the Genus Brevibacterium, J. Gen. Microbiol., 1989, vol. 135, pp. 2837–2848.Google Scholar
  7. 7.
    McBride, M.E., Ellner, K.M., Black, H.S., Clarridge, J.E., and Wolf, J.E., A New Brevibacterium sp. Isolated from Infected Genital Hair of Patients with White Piedra, J. Med. Microbiol., 1993, vol. 39, pp. 255–261.Google Scholar
  8. 8.
    Pascual, C. and Collins, M.D., Brevibacterium avium sp. nov., Isolated from Poultry, Int. J. Syst. Bacteriol., 1999, vol. 49, pp. 1527–1530.Google Scholar
  9. 9.
    Pascual, C., Collins, M.D., Funke, G., and Pitcher, D.G., Phenotypic and Genotypic Characterization of Two Brevibacterium Strains from the Human Ear: Description of Brevibacterium otitidis sp. nov., Med. Microbiol. Lett., 1996, vol. 5, pp. 113–123.Google Scholar
  10. 10.
    Wauters, G., Charlier, J., Janssens, M., and Delmee, M., Brevibacterium paucivorans sp. nov., from Human Clinical Specimens, Int. J. Syst. Evol. Microbiol., 2001, vol. 51, pp. 1703–1707.Google Scholar
  11. 11.
    Wauters, G., Avesani, V., Laffineur, K., Charlier, J., Janssens, M., Van Bosterhaut, B., and Delmee, M., Brevibacterium lutescens sp. nov., from Human and Environmental Samples, Int. J. Syst. Evol. Microbiol., 2003, vol. 53, pp. 1321–1325.Google Scholar
  12. 12.
    Kampfer, P., Differentiation of Brevibacterium Species by Electrophoretic Protein Patterns, Syst. Appl. Microbiol., 1994, vol. 17, pp. 533–535.Google Scholar
  13. 13.
    Zgurskaya, H.I., Evtushenko, L.I., Akimov, V.N., and Kalakoutskii, L.V., Rathayibacter gen. nov., Including the Species Rathayibacter rathayi comb. nov., Rathayibacter tritici comb. nov., Rathayibacter iranicus comb. nov., and Six Strains from Annual Grasses, Int. J. Syst. Bacteriol., 1993, vol. 43, pp. 143–149.Google Scholar
  14. 14.
    Evtushenko, L.I., Taran, V.V., Akimov, V.N., Kroppenstedt, R.M., Tiedje, J.M., and Stackebrandt, E., Nocardiopsis tropica sp. nov., Nocardiopsis trehalosi sp. nov., nom. rev., and Nocardiopsis dessonvilei subsp. albirubida subsp. nov., comb. nov., Int. J. Syst. Evol. Microbiol., 2000, vol. 50, pp. 73–81.Google Scholar
  15. 15.
    Streshinskaya, G.M., Shashkov, A.S., Usov, A.I., Evtushenko, L.I., and Naumova, I.B., The Cell Wall Teichoic Acids of Three Actinomycete Genera from the Order Actinomycetales, Biokhimiya, 2002, vol. 67,no. 7, pp. 939–947.Google Scholar
  16. 16.
    Versalovic, J., Schneider, M., de Bruijn, F.J., and Lupski, J.R., Genomic Fingerprinting of Bacteria Using Repetitive Sequence-based Polymerase Chain Reaction, Methods Cell. Mol. Biol., 1994, vol. 5, pp. 25–40.Google Scholar
  17. 17.
    Thompson, J.D., Higgins, D.G., and Gibson, T.J., CLUSTAL W: Improving the Sensitivity of Progressive Multiple Sequence Alignment through Sequence Weighting, Position Specific Gap Penalties, and Weight Matrix Choice, Nucleic Acids Res., 1994, vol. 22, pp. 4673–4680.Google Scholar
  18. 18.
    Jukes, T.H. and Cantor, C.R., Evolution of Protein Molecules, Mammalian Protein Metabolism, Munro, H.N., Ed., New York: Academic, 1969, pp. 21–132.Google Scholar
  19. 19.
    Van de Peer, Y. and De Wachter, R., TREECON for Windows: A Software Package for the Construction and Drawing of Evolutionary Trees for the Microsoft Windows Environment, Comput. Appl. Biosci., 1994, vol. 10, pp. 569–570.Google Scholar
  20. 20.
    Marmur, J. and Doty, P., Determination of the Base Composition of Deoxyribonucleic Acid from Its Thermal Denaturation Temperature, J. Mol. Biol., 1962, vol. 5, pp. 109–118.Google Scholar
  21. 21.
    Meyer, S.A. and Schleifer, K.H., Deoxyribonucleic Acid Reassociation in the Classification of Coagulase-Positive Staphylococci, Arch. Microbiol., 1978, vol. 117, pp. 183–188.Google Scholar
  22. 22.
    Tijssen, P., Hybridization with Nucleic Acid Probes: Part II. Probe Labeling and Hybridization Techniques, Lab. Techniq. Biochem. Mol. Biol., 1993, vol. 24, pp. 291–406.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2004

Authors and Affiliations

  • E. Yu. Gavrish
    • 1
    • 2
  • V. I. Krauzova
    • 2
  • N. V. Potekhina
    • 3
  • S. G. Karasev
    • 4
  • E. G. Plotnikova
    • 5
  • O. V. Altyntseva
    • 5
  • L. A. Korosteleva
    • 6
  • L. I. Evtushenko
    • 1
    • 2
  1. 1.Pushchino State UniversityMoscow oblastRussia
  2. 2.Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of SciencesMoscow oblastRussia
  3. 3.Moscow State University, Vorob'evy goryMoscowRussia
  4. 4.Kuban State UniversityKrasnodarRussia
  5. 5.Institute of Ecology and Genetics of Microorganisms, Ural DivisionRussian Academy of SciencesPermRussia
  6. 6.Kuban State Agrarian UniversityKrasnodarRussia

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