Molecular and Cellular Biochemistry

, Volume 266, Issue 1–2, pp 109–115 | Cite as

Effect of garlic supplementation on oxidized low density lipoproteins and lipid peroxidation in patients of essential hypertension

  • Veena Dhawan
  • Sanjay Jain


Reactive oxygen species (ROS) has been implicated in the pathogenesis of many diseases including hypertension. Therefore, certain compounds with antioxidative capacity are believed to be protective against such diseases. Some components of garlic are known to possess antioxidative properties. Therefore, in the present study we investigated the effect of short-term garlic supplementation in essential hypertensive patients (EH) on indices of oxidative stress. Twenty patients of EH as diagnosed by JNC VI criteria (Group I) and 20 age and sex-matched normotensive controls were enrolled for the study. Both groups were given garlic pearls (GP) in a dose of 250 mg per day for 2 months. Baseline samples were obtained at start of the study, i.e. 0 day, and thereafter, 2 months (follow-up). Lipids and lipoprotein subfractions, plasma-oxidized low-density lipoproteins (ox-LDL), plasma and urinary concentration of 8-iso-Prostaglandin F (8-iso-PGF) as a biomarker of oxidative stress in vivo, and the total antioxidant status (TOS) of these individuals were determined. We observed a moderate hypercholesterolemia and a significantly raised blood pressure in hypertensive patients as compared to the controls. The indices of oxidative stress, i.e. plasma ox-LDL and plasma and urinary concentration of 8-iso-PGF were significantly increased in EH group. Further, hypertensive patients had a significantly low TOS as compared to the control group. With in 2 months of GP supplementation, there was a significant decline in both systolic (SBP) and diastolic blood pressures (DBP) and a significant reduction in ox-LDL and 8-iso-PGF levels in Group I patients. Further, a moderate increase in the TOS was also observed in this group as compared to their control counterparts. These findings suggest that dietary supplementation of garlic may be beneficial in reducing blood pressure and oxidative stress in hypertensive individuals (Mol Cell Biochem 266: 109–115, 2004)

garlic hypertension lipoproteins oxidative stress 


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  1. 1.
    Harunobu A, Brenda LP, Hiromichi M, Shigeo K, Yoichi I: Recent advances on the nutritional effects associated with the use of garlic as a supplement. J Nutr 131: 955S–962S, 2001Google Scholar
  2. 2.
    Qureshi AA, Din ZZ, Abuirmeileh N, et al.: Suppression of avian hepatic lipid metabolism by solvent extracts of garlic: impact on serum lipids. J Nutr 113: 746–755.Google Scholar
  3. 3.
    Chang ML, Johnson MA: Effect of garlic on carbohydrate metabolism and lipid synthesis in rats. J Nutr 110: 931–968, 1980Google Scholar
  4. 4.
    Jain AK, Vargas R, Gotzkowsky S, Mcmohan FG: Can garlic reduce levels of serum lipids? A controlled clinical study. Am J Med 94: 632–635, 1993Google Scholar
  5. 5.
    Steiner M, Khan AH, Holbert D, Lin RI: A doubleblind crossover study in moderately hypercholesterolemic men that compared the effect of aged garlic extract and placebo administration on good lipids. Am J Clin Nutr 64: 866–870, 1996Google Scholar
  6. 6.
    Sendl A, Schliack M, Loser R, et al.: Inhibition of cholesterol synthesis in vitro by extracts and isolated compounds prepared from garlic and wild garlic. Atherosclerosis 94: 79–85, 1992Google Scholar
  7. 7.
    Gebhardt R: Multiple inhibitory effects of garlic extracts on cholesterol biosynthesis in hepatocytes. Lipids 28: 613–619, 1993Google Scholar
  8. 8.
    Agarwal KC: Therapeutic actions of garlic constituents. Med Res Rev 16: 111–124, 1996Google Scholar
  9. 9.
    Rahman K: Historical perspectives on garlic and cardiovascular disease. J Nutr 131: 977S–979S, 2001Google Scholar
  10. 10.
    Stevenson C, Pittler MH, Ernst E: Garlic for treating hypercholesterolemia Ann Intern Med 133: 420–429, 2000Google Scholar
  11. 11.
    Foushee DB, Ruffin J, Banerjee U: Garlic as a natural agent for the treatment of hypertension: A preliminary report. Cytobios 34: 145–152, 1982Google Scholar
  12. 12.
    Pedraza CJ, Tapia E, Medina Campos ON: Garlic prevents hypertension induced by chronic inhibition of nitric oxide synthesis. Life Sci 62: 71–77, 1998Google Scholar
  13. 13.
    Steiner M, Li W: Aged garlic a modulator of cardiovascular risk factors: A dose finding study on the effects of AGE on platelet function. J Nutr 131: 980S–984S, 2001.Google Scholar
  14. 14.
    Bordia A, Verma SK, Srivastava KC: Effect of garlic (Allium sativum) on blood lipids, blood sugar, fibrinogen and fibrinolytic activity in patients of coronary heart disease. Prostaglandins Leuk Essential Fatty Acids 58: 257, 1998Google Scholar
  15. 15.
    Russo C, Olivieri O, Girelli D, Faccini G, Zenari LM, Lombardi S, Corrocher R: Antioxidant status and lipid peroxidation in patients with essential hypertension. J Hypertens 16: 1267–1271, 1998Google Scholar
  16. 16.
    Dhalla NS, Temsah RM, Netticadan T: Role of oxidative stress in cardiovascular diseases. J Hypertens 18: 655–673, 2000Google Scholar
  17. 17.
    Cathcart MK, Morel DW, Chisolm GM: Monocytes and neutrophils oxidize low density lipoprotein, making it cytotoxic. J Leuko C Biol 38: 341–350, 1985Google Scholar
  18. 18.
    Steinberg D, Parthsarathy S, Carew TE, Witzum JL: Beyond Cholesterol: Modification of low density lipoprotein that increase in athero-genicity. N Engl J Med 320: 915–924, 1989Google Scholar
  19. 19.
    Darley-Usmar VM, Hogg N, O'Leary VJ, Wilson MT, Moncada S: The simultaneous generation of superoxide and nitric oxide can initiate lipid peroxidation in human lowdensity lipoprotein. Free Radic Res Commun 17: 9–20, 1992Google Scholar
  20. 20.
    Rosenfield ME, Painskiw, Yla-Herthala S, Carew TE: Macrophages, endothelial cells and lipoprotein oxidation in the pathogenesis of atherosclerosis. Toxicol Pathol 18: 566–571, 1990Google Scholar
  21. 21.
    Kuzuya M, Naito M, Funaki C, Mayashi T, Asai K, Kuzuya F: Lipid peroxide and transiton metals are required for the toxicity of oxidised low density lipoprotein to cultured endothelial cells. Biochem Biophys Acta 1096: 155–561, 1991Google Scholar
  22. 22.
    Marletha MA, Yoon PS, Iyengar R, Lear CD, Wishroik JS: Macrophage oxidation of l-arginine to nitrite and nitrate: Nitric oxide is an intermediate. Biochemistry 27: 8706–8711, 1988Google Scholar
  23. 23.
    Barton M, Haudenschild CC: Endothelium and atherogenesis: Endothelial therapy revisited. Cardiovasc Phamacol 38 (suppl): 523–525, 2001Google Scholar
  24. 24.
    Sigurdardotitir V, Fagerberg B, Hulthe J: Circulating oxidized low density lipoprotein (LDL) is associated with risk factors of the metabolic syndrome and LDL size in clinically healthy 58 year old men. J Intern Med 252: 440–447Google Scholar
  25. 25.
    Orekhov AN, Pivovarova CM, Testov VV: Garlic powder tablets reduce atherogenicity of low-density lipoproteins. A placebo-controlled double-blind study. Nutr Metab Cardiovasc Dis 6: 21–31, 1996Google Scholar
  26. 26.
    Prasad K, Laxdal VA, Yu M, Raney BL: Antioxidant activity of allicin, an active principle in garlic. Mol Cell Biochem 148: 183, 1995Google Scholar
  27. 27.
    Stephanie AD, Gordon ML, David B, Khalid R: Dietary supplementa-tion with aged garlic extract reduces plasma and urine concentrations of 8-iso-prostaglandin F2a in smoking and non-smoking men and women. J Nutr 132: 168–171, 2002Google Scholar
  28. 28.
    Marrow JD, Harris TM, Roberts LJ II: Non-cyclooxygenase oxidative formation of a series of novel prostaglandins. Analytical ramification of measurement of eicosanoids. Anal Biochem 184: 1–10, 1996Google Scholar
  29. 29.
    Delanty N, Reily AMP, Pratico D. 8-iso-PGF2a generation during coronary reperfusion. A potential quantitative marker of oxidative stress in vivo. Br JClin Pharmacol 42: 15–19, 1996Google Scholar
  30. 30.
    JNC VI — The Sixth Report of Joint National Committee on Prevention, detection, evaluation and treatment of high blood pressure. Arch Intern Med 157: 2401–2445, 1997Google Scholar
  31. 31.
    Davi G, Ciabattoni G, Consoli A, Mezzetti A, Falco A, Santarone S: In vivo formation of 8-iso-prostaglandin F and platelet activation in diabetes mellitus: Effects of improved metabolic control and vitamin E supplementation. Circulation 99: 224–229, 1999Google Scholar
  32. 32.
    Benzie Iris FF, Stein JJ: Ferric reducing antioxidant power assay: Direct measurement of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Anal Biochem 239: 15–27, 1996Google Scholar
  33. 33.
    Friedwald WT, Levy RI, Fredrickson DS: Estimation of the concentration of low density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem 18: 499–502, 1977Google Scholar
  34. 34.
    Expert Group: The Fifth Report of The Joint National Committee on detection, evaluation and treatment of high blood pressure. Arch Intern Med 153: 154–186, 1993Google Scholar
  35. 35.
    Whetton PK: Epidemiology of hypertension. Lancet 344: 101–106, 1994Google Scholar
  36. 36.
    Mader FH: Treatment of hyperlipidemia with garlic powder tablets. Arzneimittelforschung 40: 1111–1116, 1990Google Scholar
  37. 37.
    Jain AK, Vargas R, Gotzkowsky S, McMohan FG: Can garlic reduce levels of serum lipids? A controlled clinical study. Am J Med 94: 632–635, 1993Google Scholar
  38. 38.
    Pedraza-Chaverri J, Tapia E, Medina Campus ON, et al: Garlic prevents hypertension induced by chronic inhibition of NO synthesis. Life Sci 62: 71–72, 1998Google Scholar
  39. 39.
    Siegel G, Nuck R. Schnalke F, Michel F.: Molecular evidences for phy-topharmacological K channel opening by garlic in human vascular smooth muscle cell membranes. Phytother Res 12: S149–S151, 1998Google Scholar
  40. 40.
    Suetsuna K: Isolation and characterization of angiotensin I converting enzyme inhibitor dipeptides derived from Allium sativum L. (garlic). J Nutr Biochem 9: 415–419, 1998Google Scholar
  41. 41.
    Silaqy CA, Neil HAW. A meta analysis of the effect of garlic on blood pressure. J Hypertens 12: 463–468, 1994Google Scholar
  42. 42.
    Ide N, Nelson A, Lau BH: Aged garlic extract and its constituents inhibit Cu2+-induced oxidative modification of low density lipoprotein. Planta Med 63: 263–264, 1997Google Scholar
  43. 43.
    Phelps S, Harris W: Garlic supplementation and lipoprotein oxidative susceptibility. Lipids 28: 475–477, 1993Google Scholar
  44. 44.
    Reilly M, Delanty N, Lawson JA, Fitzgerald GA: Modulation of chronic oxidant stress in vivo in chronic cigarette smokers. Circulation 94: 19–25, 1996Google Scholar
  45. 45.
    Reilly MP, Pratico D, Delanty N, Delanty N, Di Minno G, Tremoli E, Rader D, et al.: Increased formation of distinct F2 isoprostane in hypercholesterolemia. Circulation 28: 22–28, 1998Google Scholar
  46. 46.
    Gopaul NK, Anggard EE, Mallet AI, Betteridge DJ, Walff SP, Naurooz-Zadeh J: Plasma-epi-PGF levels are elevated in individuals with non-insulin dependent diabetes mellitus. FEBS Lett 368: 225–229, 1995Google Scholar
  47. 47.
    Gopaul NK, Halliwell B, Angard FE: Measurement of plasma F2 isoprostane as an index of lipid peroxidation does not appear to be confounded by diet. Free Radic Res 33: 115–127, 2000Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Veena Dhawan
    • 1
  • Sanjay Jain
    • 2
  1. 1.Department of Experimental Medicine & BiotechnologyPostgraduate Institute of Medical Education and ResearchChandigarhIndia.
  2. 2.Department of Internal MedicinePostgraduate Institute of Medical Education and ResearchChandigarhIndia

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