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Molecular and Cellular Biochemistry

, Volume 266, Issue 1–2, pp 57–63 | Cite as

Aspirin-induced apoptosis in Jurkat cells is not mediated by peroxisome proliferator-activated receptor delta

  • José M. López
  • Manuel A. Fernández
  • Maria Piqué
  • Joan Gil
Article

Abstract

Nonsteroidal antiinflammatory drugs may induce apoptosis via inhibition of peroxisome proliferator-activated receptor delta (PPARδ) activity. Here we analyze the role of PPARδ in aspirin-induced apoptosis of Jurkat cells, which, together with other lymphoid cell lines, express PPARδ mRNA. Aspirin increased PPARδ mRNA levels in Jurkat cells, but decreased the activity of both PPARδ and PPARα/γ, assayed using the luciferase reporter constructs DRE and ACO, respectively. The DNA binding of PPARδ was not affected by 10 mM aspirin, which induces apoptosis in Jurkat cells. Moreover, neither addition of a specific ligand of PPARδ nor transient transfection of PPARδ expression vectors protected Jurkat cells from aspirin-induced apoptosis. These results indicate that PPARδ is not involved in aspirin-induced apoptosis. Therefore, the mechanism by which aspirin mediates cell death in Jurkat cells remains unknown (Mol Cell Biochem 266: 57–63, 2004)

apoptosis aspirin Jurkat lymphoid cells NSAIDs PPAR 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • José M. López
    • 1
    • 2
  • Manuel A. Fernández
    • 1
    • 3
  • Maria Piqué
    • 1
    • 2
  • Joan Gil
    • 1
  1. 1.Unitat de Bioquímica, Departament de Ciències Fisiològiques IIUniversitat de Barcelona, Campus de BellvitgeL'HospitaletSpain
  2. 2.Centre de Regulació GenòmicaPrograma d'Expressió Gènica, Pg. Maritim 37-49BarcelonaSpain.
  3. 3.Departament de Biologia Celular i Anatomia Patològica, Institut d' Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Facultat de MedicinaUniversitat de BarcelonaBarcelonaSpain

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