Advertisement

Molecular and Cellular Biochemistry

, Volume 261, Issue 1, pp 137–142 | Cite as

Effects of diabetes mellitus on salivary secretion and its composition in the human

  • Antonio D. Mata
  • Duarte Marques
  • Sara Rocha
  • Helena Francisco
  • Carolina Santos
  • Maria F. Mesquita
  • Jaipaul Singh
Article

Abstract

This study investigated the effects of diabetes mellitus (types I and II) on human salivary gland function compared to healthy age-matched controls. The results have shown that both type I and type II diabetic patients secrete significantly (p < 0.05) less resting and stimulated saliva compared to healthy age-matched controls (AMC). It was also found that the diabetic patients have an increased resting and stimulated salivary protein concentration compared to healthy participants. However, the secretory capacity (stimulated minus resting values) was markedly reduced compared to controls. The level of calcium (Ca2+) in the saliva of diabetic patients was significantly (p < 0.05) elevated compared to the AMC. In contrast, the levels of magnesium (Mg2+), zinc (Zn2+) and potassium (K+) in the saliva of diabetic patients were significantly (p < 0.05) reduced compared to the values obtained in AMC. These results indicate that diabetes mellitus can lead to marked dysfunction of the secretory capacity of the salivary glands. In these patients a modified fluid, organic and inorganic salivary secretion may be responsible for the increased susceptibility to oral infections and impaired wound healing described by others in the literature. (Mol Cell Biochem 261: 137–142, 2004)

diabetes mellitus human saliva protein calcium magnesium zinc potassium 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Mandel ID: Salivary diagnosis: More than a lick and a promise. J Am Dent Assoc 124: 85–87, 1993PubMedGoogle Scholar
  2. 2.
    Pellerin C, Pellat B: La salive. In: Biochimie Odonto Stomatologique. Masson, Paris 1986, pp 33–54Google Scholar
  3. 3.
    Kaufman E, Lamster IB: The diagnostic applications of saliva—a review. Crit Rev Oral Biol Med 13(2): 197–212, 2002PubMedGoogle Scholar
  4. 4.
    Drobitch RK, Svensson CK: Therapeutic drug monitoring in saliva. An update. Clin Pharmacokinet 23: 365–379, 1992PubMedGoogle Scholar
  5. 5.
    Fox PC: Salivary monitoring in oral diseases. Ann NY Acad Sci 694: 234–237, 1993PubMedGoogle Scholar
  6. 6.
    Davies PB: Pathophysiology of cystic fibrosis with emphasis on salivary gland involvement. J Dent Res 66: 667–671, 1987Google Scholar
  7. 7.
    Samuel NM, Chandrasekaran A, Paul SA: Detection of antibodies to HIV-1 in serum and saliva. J Assoc Physicians India 45(4): 280–282, 1997PubMedGoogle Scholar
  8. 8.
    Daniels TE, Fox PC: Salivary and oral components of Sjögren's syndrome. Rheum Dis Clin North Am 18: 571–589, 1992PubMedGoogle Scholar
  9. 9.
    Nechifor M, Teslariu E, Mindreci I, Mihailovici S, Matei V, Filip C, Miftode M, Danila GH: The influence of magnesium, chromium and copper in alloxan-induced diabetic rats. In: Advances in Magnesium Research Nutrition and Health. Y. Rayssiguier, A. Mazur, J. Durlach (eds). John Libbey, London, 2001, pp 375–379Google Scholar
  10. 10.
    Twetman S, Johansson I, Birkhed D, Nederfors T: Caries incidence in young type 1 diabetes mellitus patients in relation to metabolic control and caries-associated risk factors. Caries Res 36(1): 31–35, 2002CrossRefPubMedGoogle Scholar
  11. 11.
    Chavez EM, Borrell LN, Taylor GW, Ship JA: A longitudinal analysis of salivary flow in control participants and older adults with type 2 diabetes. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 91(2): 166–173, 2002Google Scholar
  12. 12.
    Navazesh M: Salivary gland hypofunction in ederly patients. J Calif Dent Assoc 22: 62–68, 1992Google Scholar
  13. 13.
    Lowry OH, Roseborough NJ, Forr AL, Randall RJ: Protein measurement with folin phenol reagent. J Biol Chem 193: 265–275, 1951PubMedGoogle Scholar
  14. 14.
    Markopoulos AK, Belazi M: Histopathological and immunohistochemical features of the labial salivary glands in children with type 1 diabetes. J Diabetes Complications 12: 39–42, 1998CrossRefPubMedGoogle Scholar
  15. 15.
    Ship JA, Pillemer SR, Baum BJ: Xerostomia and the geriatric patient. J Am Geriatr Soc 50(3): 535–543, 2002CrossRefPubMedGoogle Scholar
  16. 16.
    Oxford GE, Tayari L, Barfoor MD, Peck AB, Tanaka Y, Humphreys-Beher MG: Salivary EGF levels reduced in diabetic patients. J Diabet Complications 14: 140–145, 2000Google Scholar
  17. 17.
    Belce A, Uslu E, Kucur M, Umut M, Ipbuker A, Seymen HO: Evaluation of salivary sialic acid level and Cu-Zn superoxide dismutase activity in type 1 diabetes mellitus. Tohoku J Exp Med 192(3): 219–225, 2000CrossRefPubMedGoogle Scholar
  18. 18.
    Tosiello L: Hypomagnesemia and diabetes mellitus. Arch Int Med 156: 1143–1148, 1996Google Scholar
  19. 19.
    Leeuw ID: Magnesium and diabetes: Is magnesium depletion a risk factor for complications in type 1 diabetic patients. In: Advances in Magnesium Research Nutrition and Health. Y. Rayssiguier, A. Mazur, J. Durlach (eds). John Libbey, London, 2001, pp 369–374Google Scholar
  20. 20.
    Mata AD, Marques D, Geraldes M, Rocha S, Mesquita MF, Singh J: Acetylcholine evoked salivary secretion: Role of extracellular magnesium on acetylcholine evoked secretory responses in the isolated rat submandibular cell glands. In: Advances in Magnesium Research Nutrition and Health. Y. Rayssiguier, A. Mazur, J. Durlach (eds). John Libbey, London, 2001, pp 93–96Google Scholar
  21. 21.
    Yago MD, Mata AD, Mañas M, Singh J: Effect of extracellular magnesium on nerve mediated and acetylcholine-evoked secretory responses in the isolated rat parotid gland. Exp Physiol 87(3): 321–326, 2002CrossRefPubMedGoogle Scholar
  22. 22.
    Jin Y, Hak-Kong H: Supragengival calculus: Formation and control. Crit Rev Oral Biol Med 13(5): 426–441, 2002PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Antonio D. Mata
    • 1
  • Duarte Marques
    • 1
  • Sara Rocha
    • 1
  • Helena Francisco
    • 1
  • Carolina Santos
    • 1
  • Maria F. Mesquita
    • 1
  • Jaipaul Singh
    • 2
  1. 1.Department of Oral BiologyISCS-SulMonte CaparicaPortugal
  2. 2.Department of Biological SciencesUniversity of Central LancashirePrestonEngland, UK

Personalised recommendations