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Molecular and Cellular Biochemistry

, Volume 260, Issue 1, pp 103–110 | Cite as

Modulation of intracellular calcium concentrations and T cell activation by prickly pear polyphenols

  • Virginie Aires
  • Sylvie Adote
  • Aziz Hirchami
  • Kabirou Moutairou
  • Es-Saddik E. Boustani
  • Naim A. Khan
Article

Abstract

Opuntia ficus indica(prickly pear) polyphenolic compounds (OFPC) triggered an increase in [Ca2+]i in human Jurkat T-cell lines. Furthermore, OFPC-induced rise in [Ca2+]i was significantly curtailed in calcium-free buffer (0% Ca2+) as compared to that in 100% Ca2+ medium. Preincubation of cells with tyrphostin A9, an inhibitor of Ca2+ release-activated Ca2+(CRAC) channels, significantly diminished the OFPC-induced sustained response on the increases in [Ca2+]i. Lanthanum and nifedipine, the respective inhibitors of voltage-dependent and L-type calcium channels, failed to curtail significantly the OFPC-induced calcium response. As OFPC still stimulated increases in [Ca2+]i in 0% Ca2+ medium, the role of intracellular calcium was investigated. Hence, addition of thapsigargin (TG), an inhibitor of Ca2+-ATPase of the endoplasmic reticulum (ER), during the OFPC-induced peak response exerted an additive effect, indicating that the mechanism of action of these two agents are different. Furthermore, U73122, an inhibitor of IP3 production, completely abolished increases in [Ca2+]i, induced by OFPC, suggesting that these polyphenols induce the production of IP3 that recruits calcium from ER pool. Polyphenolic compounds do act extracellularly as addition of fatty acid-free bovine serum albumin (BSA) significantly diminished the rise in [Ca2+]i evoked by the formers. OFPC also induced plasma membrane hyperpolarisation which was reversed by addition of BSA. OFPC were found to curtail the expression of IL-2 mRNA and T-cell blastogenesis. Together these results suggest that OFPC induce increases in [Ca2+]i via ER pool and opening of CRAC channels, and exert immunosuppressive effects in Jurkat T-cells.

polyphenols Jurkat T-cells intracellular calcium prickly pear 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Virginie Aires
    • 1
  • Sylvie Adote
    • 2
  • Aziz Hirchami
    • 1
  • Kabirou Moutairou
    • 2
  • Es-Saddik E. Boustani
    • 3
  • Naim A. Khan
    • 1
  1. 1.UPRES Lipides and NutritionUniversité de BourgogneDijonFrance
  2. 2.FASTUniversité d'Abomé CalaviCotonouBenin
  3. 3.Laboratoire de Biochimie, Unité de Recherche Nutrition-Santé, Faculté des Sciences SemlaliaUniversité Cadi AyyadMarrakechMorocco

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