Journal of Fluorescence

, Volume 14, Issue 4, pp 401–405

Quenched Emission of Fluorescence by Ligand Functionalized Gold Nanoparticles



A fluorescence-based detection scheme that uses ligand functionalized gold nanoparticles is proposed. The transduction scheme is based on the strong quenching of the fluorescence emission exerted by metallic surfaces on fluorophores positioned in their immediate vicinity (<5 nm). Binding of fluorophore-labeled anti-biotin to biotinylated gold nanoparticles resulted in decreased fluorescence emission intensity. Subsequent competitive dissociation of labeled anti-biotin with D-biotin resulted in increased fluorescence emission intensity. These interactions occurred by means of specific molecular recognition because when the binding sites of anti-biotin were saturated with D-biotin prior to interaction with the gold nanoparticles; changes in the fluorescence emission intensity were not observed.

Gold nanoparticles self-assembled monolayers surface modification fluorescence energy transfer 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    M. A. Hayat (1989). Colloidal Gold: Principles, Methods, and Applications, Academic Press, San Diego, CA.Google Scholar
  2. 2.
    J. J. Storhoff, R. Elghanian, R. C. Mucic, C. A. Mirkin, and R. L. Letsinger (1998). One-pot colorimetric differentiation of polynucleotides with single base imperfections using gold nanoparticle probes. J. Am. Chem. Soc. 120, 1959-1964.Google Scholar
  3. 3.
    C. A. Mirkin, R. L. Letsinger, R. C. Mucic, and J. J. Storhoff (1996). A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature 382, 607-609.Google Scholar
  4. 4.
    J. H. Kim, J. H. Cho, G. S. Cha, C. W. Lee, H. B. Kim, and C. W. Paek (2000). Conductimetric membrane strip immunosensor with polyaniline-bound gold colloids as signal generator. Biosens.Bioelectron. 14, 907-915.Google Scholar
  5. 5.
    O. D. Velev and E. W. Kaler (1999). In situ assembly of colloidal particles into miniaturized biosensors. Langmuir 15, 3693-3698.Google Scholar
  6. 6.
    H. Li, Y. Y. Luk, and M. Mrksich (1999). Catalytic asymmetric dihydroxylation by gold colloids functionalized with self-assembled monolayers. Langmuir 15, 4957-4959.Google Scholar
  7. 7.
    C. P. Collier, T. Vossmeyer, and J. R. Heath (1998). Nanocrystal Superlattices. Annu. Rev. Phys. Chem. 49, 371-404.Google Scholar
  8. 8.
    P. Avouris and B. N. J. Persson (1984). Excited states at metal surfaces and their non-radiative relaxation. J. Phys. Chem. 88, 837-848.Google Scholar
  9. 9.
    B. N. J. Persson and S. Anderson (1984). Dynamical processes at surfaces: Excitation of electron-hole pairs. Phys. Rev. B 29, 4382-4394.Google Scholar
  10. 10.
    G. Cnossen, K. E. Drabe, and D. A. Wiersma (1993). Fluorescence properties of submonolayers of rhodamine 6G in front of a mirror. J. Chem. Phys. 98, 5276-5280.Google Scholar
  11. 11.
    J. R. Lakowicz (2001). Radiative decay engineering: Biophysical and Biomedical applications. Anal. Biochem. 298, 1-24.Google Scholar
  12. 12.
    K. Sokolov, G. Chumanov, and T. M. Cotton (1998). Enhancement of molecular fluorescence near the surface of colloidal metal films. Anal. Chem. 70, 3898-3905.Google Scholar
  13. 13.
    T. Liebermann and W. Knoll (2000). Surface-plasmon field-enhanced fluorescence spectroscopy. Colloids Surf. A 171, 115-130.Google Scholar
  14. 14.
    I. Gryczynski, J. Malicka, Y. Shen, Z. Gryczynski, and J. R. Lakowicz (2002). Multiphoton excitation of fluorescence near metallic particles: Enhanced and localized excitation. J. Phys.Chem. B 106, 2191-2195.Google Scholar
  15. 15.
    V. H. Pérez-Luna, S. Yang, E. M. Rabinovich, T. Buranda, L. A. Sklar, P. D. Hampton, and G. P. López (2002). Fluorescence biosensing strategy based on energy transfer between fluorescently labeled receptors and a metallic surface. Biosens. Bioelectron. 17, 71-78.Google Scholar
  16. 16.
    A. Ulman (1991). An Introduction to Ultrathin Organic Films: From Langmuir-Blodgett to Self Assembly, Academic Press, Boston, MA.Google Scholar
  17. 17.
    K. Aslan and V.H. Pérez-Luna (2002). Surface modification of colloidal gold by chemisorption of alkanethiols in the presence of a nonionic surfactant. Langmuir 18, 6059-6065.Google Scholar
  18. 18.
    D. J. Maxwell, J. R. Taylor, and S. Nie (2002). Self-assembled nanoparticle probes for recognition and detection of biomolecules. J. Am. Chem. Soc. 124, 9606-9612.Google Scholar
  19. 19.
    A. Aguila and R.W. Murray (2000). Monolayer-protected clusters with fluorescent dansyl ligands. Langmuir 16, 5949-5954.Google Scholar

Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  1. 1.Department of Chemical and Environmental EngineeringIllinois Institute of TechnologyChicago

Personalised recommendations