Journal of Chemical Ecology

, Volume 30, Issue 5, pp 965–976 | Cite as

Laboratory and Field Responses of the Mosquito, Culex quinquefasciatus, to Plant-Derived Culex spp. Oviposition Pheromone and the Oviposition Cue Skatole

  • Timothy O. Olagbemiro
  • Michael A. Birkett
  • A. Jennifer Mordue (Luntz)
  • John A. Pickett
Article

Abstract

Laboratory and field studies were conducted on the oviposition behavior of the pathogen-vectoring mosquito, Culex quinquefasciatus, in response to the oviposition pheromone 6-acetoxy-5-hexadecanolide, produced from a renewable plant resource, Kochia scoparia (Chenopodiaceae) (plant-derived pheromone, PDP), and via an established synthetic route (synthetic oviposition pheromone, SOP). Responses to the oviposition cue skatole (3-methylindole), presented individually and in combination with the plant-derived and synthetic oviposition pheromone, were also studied. Both laboratory and field assays showed that PDP and SOP were equally attractive. Synergistic effects were observed with one combination of PDP and skatole combinations in laboratory assays. Synergy was also observed under field conditions. SOP and skatole combinations showed additive effects in laboratory assays, but were not tested in field bioassays. Although synergism has been previously demonstrated with combinations of SOP and polluted waters, the work presented here is the first example of synergy between a specific oviposition attractant and the oviposition pheromone. Furthermore, the efficacy of mosquito pheromone produced from a cheap, renewable botanical source has been demonstrated.

Culex quinquefasciatus oviposition pheromone 6-acetoxy-5-hexadecanolide Kochia scoparia renewable resource skatole synergism 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

REFERENCES

  1. Beehler, J. W., Millar, J. G., and Mulla, M. S. 1994. Field evaluation of synthetic compounds mediating oviposition in Culex mosquitoes (Diptera, Culicidae). J. Chem. Ecol. 20:281–291.Google Scholar
  2. Blackwell, A., Mordue (Luntz), A. J., Hansson, B. S., Wadhams, L. J., and Pickett, J. A. 1993. A behavioural and electrophysiological study of oviposition cues for Culex quinquefasciatus. Physiol. Entomol. 18:343–348.Google Scholar
  3. Dawson, G. W., Mudd, A. L., Pickett, J. A., Pile, M. M., and Wadhams, L. J. 1990. Convenient synthesis of mosquito oviposition pheromone and a highly fluorinated analog retaining biological activity. J. Chem. Ecol. 16:1779–1789.Google Scholar
  4. Donaldson, L. J. 2002. Getting Ahead of the Curve: A Strategy for Combating Infectious Diseases. UK Department of Health report, London.Google Scholar
  5. Edwards, F. W. 1942. Mosquitoes of the Ethiopian Region III—Culicine Adults and Pupae. Adlard, London.Google Scholar
  6. Gillett, J. D. 1972. Common African Mosquitoes and Their Medical Importance. William Heinemann Medical Books, London.Google Scholar
  7. Jonsson, N. N. and Reid, S. W. J. 2000. Global climate change and vector-borne diseases. Vet. J. 160:87–89.Google Scholar
  8. Laurence, B. R. and Pickett, J. A. 1982. erythro-6-Acetoxy-5-hexadecanolide, the major component of an oviposition attractant pheromone. Chem. Commun. 1:59–60.Google Scholar
  9. Mboera, L. E. G., Mdira, K. Y., Salum, F. M., Takken, W., and Pickett, J. A. 1999. The influence of synthetic oviposition pheromone and volatiles from soakage pits and grass infusions upon oviposition site-selection of Culex mosquitoes in Tanzania. J. Chem. Ecol. 25:1855–1865.Google Scholar
  10. Mboera, L. E. G., Takken, W., Mdira, K. Y., Chuwa, G. J., and Pickett, J. A. 2000a. Oviposition and behavioural responses of Culex quinquefasciatus to skatole and synthetic oviposition pheromone in Tanzania. J. Chem. Ecol. 26:1193–1203.Google Scholar
  11. Mboera, L. E. G., Takken, W., Mdira, K. Y., and Pickett, J. A. 2000b. Sampling gravid Culex quinquefasciatus (Diptera, Culicidae) using traps baited with synthetic oviposition pheromone and grass infusions in Tanzania. J. Med. Entomol. 33:172–176.Google Scholar
  12. Millar, J. G., Chaney, J. D., Beehler, J. W., and Mulla, M. S. 1994. Interaction of the Culex quinquefasciatus egg raft pheromone with a natural chemical associated with oviposition sites. J. Am. Mosq. Control Assoc. 10:374–379.Google Scholar
  13. Millar, J. G., Chaney, J. D., and Mulla, M. S. 1992. Identification of oviposition attractants for Culex quinquefasciatus from fermented Bermuda grass infusion. J. Am. Mosq. Control Assoc. 8:11–17.Google Scholar
  14. Mordue(Luntz), A. J., Blackwell, A., Hansson, B., Wadhams, L. J., and Pickett, J. A. 1992. Behavioural and physiological evaluation of oviposition attractants for Culex quinquefasciatus Say (Diptera: Culicidae). Experientia 48:1109–1111.Google Scholar
  15. Olagbemiro, T. O., Birkett, M. A., Mordue (Luntz), A. J., and Pickett, J. A. 1999. Production of (5R,6S)-6-acetoxy-5-hexadecanolide, the mosquito oviposition pheromone, from the seed oil of the Summer Cypress plant, Kochia scoparia (Chenopodiaceae). J. Agric. Food Chem. 47:3411–3415.Google Scholar
  16. Otieno, W. A., Onyango, T. O., Pile, M. M., Laurence, B. R., Dawson, G. W., Wadhams, L. J., and Pickett, J. A. 1988. A field trial of the synthetic oviposition pheromone with Culex quinquefasciatus Say (Diptera, Culicidae) in Kenya. Bull. Ent. Res. 78:463–470.Google Scholar
  17. Pickett, J. A. and Woodcock, C. M. 1996. The role of mosquito olfaction in oviposition site location and in the avoidance of unsuitable hosts, pp. 109-123, in R. G.Bock and G.Cardew (eds.). Olfaction in Mosquito-Host Interactions. Wiley, Chichester, UK.Google Scholar
  18. Pinheiro, F. 1997. Global situation of dengue and dengue haemorrhagic fever, and its emergence in the Americas. World Health Stat. Q. 3-4:161–169.Google Scholar
  19. Reiter, P. 1983. A portable battery-powered trap for collecting gravid Culex mosquitoes. Mosq. News 43:496–498.Google Scholar
  20. Reiter, P. 1986. A standardized procedure for the quantitative surveillance of certain Culex mosquitoes by egg raft collection. J. Am. Mosq. Control Assoc. 2:219–221.Google Scholar
  21. Riesen, W. K., Milby, M. M., Presser, S. B., and Hardy, J. L. 1992. Ecology of mosquito and St. Louis encephalitis virus in Los Angeles basin of California. J. Med. Entomol. 29:582–598.Google Scholar
  22. Turell, M. J., Sardelis, M. R., O'Guinn, M. L., and Dohm, D. J. 2002. Potential vectors of West Nile virus in North America, pp. 241–252, in J. S.McKenzie, A. D. T.Barrett, and V.Deubel (eds.). Current Topics in Microbiology and Immunology. Japanese Encephalitis and West Nile Viruses. Springer, Berlin.Google Scholar
  23. WHO. 1992. Lymphatic Filariasis: The Disease and Its Control. WHO report, Geneva.Google Scholar
  24. WHO/CTD. 1998. Malaria Prevention and Control. WHO report, Geneva.Google Scholar

Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Timothy O. Olagbemiro
    • 1
  • Michael A. Birkett
    • 2
  • A. Jennifer Mordue (Luntz)
    • 3
  • John A. Pickett
    • 2
  1. 1.Department of ChemistryAbubakar Tafewa Balewa UniversityBauchiNigeria
  2. 2.Biological Chemistry DivisionRothamsted ResearchHarpenden, HertsUnited Kingdom
  3. 3.Department of ZoologyUniversity of AberdeenUnited Kingdom

Personalised recommendations