Journal of Chemical Ecology

, Volume 23, Issue 7, pp 1661–1673 | Cite as

Analysis of EPG Recorded Probing by Cabbage Aphid on Host Plant Parts with Different Glucosinolate Contents

  • B. Gabrys
  • W. F. Tjallingii
  • T. A. Van Beek


Probing behavior of adult apterous Brevicoryne brassicae (L.) was monitored with the electical penetration graph (EPG) technique on Sinapis alba L. Old, young, and mature leaves, as well as the generally preferred stems were compared with respect to probing activity and glucosinolate content. Compared to leaves, EPGs on stems showed much prolonged initial probes, which more often led to phloem-phase waveforms and successively to sustained phloem ingestion. However, reaching the first phloem phase within a probe was delayed on stems so that the time to the first phloem phase within the experiment was rather similar on the different plant parts. This suggests that superficial (epidermal and mesophyll) factors are more favorable in stems than in leaves but for deeper tissues, i.e., mesophyll and vascular bundle cells, the situation seems reversed, except for the phloem sieve elements, which seemed more or at least equally suitable. 3-Butenyl, p-hydroxybenzyl, and benzyl glucosinolates were found in all parts. It is concluded that further research on distribution and compartmentation of glucosinolates within plant tissues is needed for a better understanding of the correlations found with some B. brassicae probing parameters.

Aphids Homoptera Sinapis Cruciferae allelochemicals feeding electrical monitoring electrical penetration graph technique 


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  1. BETZ, J. M., and PAGE, S. W. 1990. Liquid chromatographic method for the determination of intact, non-derivatized glucosinolates from Brassicaceae, pp. 103–104, in Proceedings of the Symposium Biology and Chemistry of Active Natural Substances. Bonn, July 1990.Google Scholar
  2. BLACKMAN, R. L., and EASTOP, V. F. 1985. Aphids on the World's Crops: An Identification Guide. John Wiley & Sons, New York, pp. 413.Google Scholar
  3. CAILLAUD, C. M., PIERRE, J. S., CHAUBET, B., and DIPIETRO, J. P. 1995. Analysis of wheat resistance to the cereal aphid Sitobion avenae using electrical penetration graphs and flow charts combined with correspondence analysis. Entomol. Exp. Appl. 75:9–18.Google Scholar
  4. CHEW, F. S. 1988. Searching for defensive chemistry in the Cruciferae, or, do glucosinolates always control interactions of Cruciferae with their potential herbivores and symbionts? No!, pp. 81–112, in K. C. Spencer (ed.), Chemical Mediation of Coevolution. Academic Press, San Diego.Google Scholar
  5. COLE, R. A. 1994. Locating a resistance mechanism to the cabbage aphid in two wild brassicas. Entomol. Exp. Appl. 71:32–31.Google Scholar
  6. DODD, G. D., andVAN EMDEN, H. F. 1979. Shifts in host plant resistance to the cabbage aphid (Brevicoryne brassicae) exhibited by brussels sprout plant. Ann. Appl. Biol. 91:251–262.Google Scholar
  7. FENWICK, G. R., HEANEY R. K., and MULLIN, W. J. 1983. Glucosinolates and their breakdown products in food and foodplants. CRC Crit. Rev. Food Sci. Nutr. 18:123–202.Google Scholar
  8. GABRYS, B. 1991. Distribution of Brevicoryne brassicae and Myzus persicae on yellow mustard plant, pp. 303–305 in Proceedings, Conference Insect Chemical Ecology, Tabor, 1990, Academia, Prague, and SPB Academic Publishers, The Hague.Google Scholar
  9. GINOVICH, A., and NIEMEYER, H. M. 1991. Hydroxyamic acids affecting barley yellow dwarf virus transmission by the aphid Rhopalosiphum padi. Entomol. Exp. Appl. 59:79–85.Google Scholar
  10. KLINGAUF, F. A. 1987. Feeding, adaptation and excretion, pp. 225–248, in A. K. Minks and P. Hareewijn (eds.). Aphids, Their Biology, Natural Enemies and Control, Vol. 2A. Elsevier, Amsterdam.Google Scholar
  11. MATILE, P. 1984. Das toxische Kompartiment der Pflanzenzelle. Naturwissenschaften 71:18–24.Google Scholar
  12. MERRITT, S. Z. 1996. Within-plant variation in concentrations of amino acids, sugar, and sinigrin in phloem sap of black mustard, Brassica nigra (L.) Koch (Curciferae). J. Chem. Ecol. 22:1133–1145.Google Scholar
  13. MILES, P. W., ASPINALL, D., and ROSENBERG, L. 1982. Performance of the cabbage aphid, Brevicoryne brassicae (L.), on water stressed rape plants, in relation to changes in their chemical composition. Aust. J. Zool. 30:337–345.Google Scholar
  14. MONTLLOR, C. B., and TJALLINGII, W. F. 1989. Stylet penetration by two aphid species on susceptible and resistant lettuce. Entomol. Exp. Appl. 52:103–111.Google Scholar
  15. NAULT, L. R., and STYER W. E. 1972. Effects of sinigrin on host selection by aphids. Entomol. Exp. Appl. 15:423–437.Google Scholar
  16. PRADO, E., and TJALLINGII, W. F. 1993. Aphid activities during sieve element punctures, pp. 109–112, in P. Kindlmann and A. F. G. Dixon (eds.). Critical Issues in Aphid Biology. Proceedings, 4th International Symposium on Aphids, Ceske Budejovice.Google Scholar
  17. TJALLINGII, W. F. 1985. Membrane potentials as an indication for plant cell penetration by aphid stylets. Entomol. Exp. Appl. 38:187–194.Google Scholar
  18. TJALLINGII, W. F. 1988. Electrical recording of stylet penetration activities, pp. 95–108, in A. K. Minks and P. Harrweijn (eds.). Aphids, Their Biology, Natural Enemies and Control, Vol. 2B. Elsevier, Amsterdam.Google Scholar
  19. TJALLINGII, W. F. 1990. Stylet penetration parameters from aphids in relation to host-plant resistance. Symp. Biol. Hung. 39:411–419.Google Scholar
  20. TJALLINGII, W. F. 1995. Aphid-plant interactions: What goes on in the depth of the tissues? Proc. Exp. Appl. Entomol. 6:163–169.Google Scholar
  21. TJALLINGII, W. F., and HOGEN ESCH, T. 1993. Fine structure of aphid stylet routes in plant tissues in correlation with EPG signals. Physiol. Entomol. 18:317–328.Google Scholar
  22. TJALLINGII, W. F., and MAYORAL, A. 1992. Criteria for host-plant acceptance by aphids, pp. 280–282, in S. B. J. Menken, J. H. Visser, and P. Harrewijn (eds.). Proceedings 8th International Symposium on Insect-Plant Relationships. Kluwer Academic Publishers, Dordrecht.Google Scholar
  23. VAN EMDEN, H. F. 1978. Insects and secondary plant substances—an altemative viewpoint with special reference to aphids, pp. 309–323, in J. B. Harborne (ed.). Biochemical Aspects of Plant and Animal Coevolution. Academic Press, London.Google Scholar
  24. VAN EMDEN, H. F., and BASHFORD, M. A. 1969. A comparison of the reproduction of Brevicoryne brassicae and Myzus persicae in relation to soluble nitrogen concentration and leaf age (leaf position) in the Brussels sprout plant. Entomol. Exp. Appl. 12:351–364.Google Scholar
  25. VAN HELDEN M., and TJALLINGII, W. F. 1993. Tissue localization of lettuce resistance to the aphid Nasonovia ribisnigri using electrical penetration graphs. Entomol. Exp. Appl. 68:269–278.Google Scholar
  26. VAN HOOF, H. A. 1958. An investigation of the biological transmission of a nonpersistent virus, pp. 81–112, in A. Van Putten and B. Oortmeijer. Alkmaar. Academic Press, New York.Google Scholar
  27. WEBER, G., OSWALD, S., and ZOLLNER, U. 1986. Suitability of rape cultivars with a different glucosinolate content for Brevicoryne brassicae (L.) and Myzus persicae (Sulzer) (Hemiptera, Aphididae). J. Plant. Dis. Prot. 93:113–124.Google Scholar
  28. WENSLER, R. J. D. 1962. Mode of host selection by an aphid. Nature 195:830–831.Google Scholar

Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • B. Gabrys
    • 1
  • W. F. Tjallingii
    • 2
  • T. A. Van Beek
    • 3
  1. 1.Department of Agricultural EntomologyAgricultural UniversityWrocławPoland
  2. 2.Department of EntomologyWageningen Agricultural UniversityWageningenThe Netherlands
  3. 3.Department of Organic Chemistry, Phytochemical SectionWageningen Agricultural UniversityWageningenThe Netherlands

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