Journal of Clinical Immunology

, Volume 24, Issue 1, pp 74–85

Increased Expression of CCL20 in Human Inflammatory Bowel Disease

  • Arthur Kaser
  • Othmar Ludwiczek
  • Sandra Holzmann
  • Alexander R. Moschen
  • Günter Weiss
  • Barbara Enrich
  • Ivo Graziadei
  • Stefan Dunzendorfer
  • Christian J. Wiedermann
  • Elisabeth Mürzl
  • Eveline Grasl
  • Zerina Jasarevic
  • Nikolaus Romani
  • Felix A. Offner
  • Herbert Tilg


Inflammatory bowel disease (IBD) constituting Crohn's disease (CD) and ulcerative colitis (UC) is related to a dysregulated T cell response. CCL20 attracts memory T lymphocytes and dendritic cells. We asked whether CCL20 expression is altered in IBD. Colonic biopsies were obtained from 114 subjects with IBD, non-IBD colitis, irritable bowel syndrome, and healthy controls. CCL20 and CCR6 mRNA expression was measured by Taqman-PCR, and protein secretion from colonic explant cultures (CEC) and its regulation by TNF-α by ELISA. CCL20, CCR6, and Langerin were identified by immunohistochemistry and immunofluorescence. CCL20 mRNA and protein were severalfold increased in involved CD and UC but not in non-IBD colitis. TNF-α increased and anti-TNF-α decreased CCL20 release in healthy control CEC but not in involved IBD colonic specimens. CCL20 localized to follicle-associated epithelium, and CCR6 to the adjacent mantle zone of lymphoid follicles. Furthermore, abundant numbers of Langerin+ immature dendritic cells were identified in the subepithelial space of IBD specimens. CCL20 might regulate the attraction of T lymphocytes and dendritic cells in IBD.

IBD chemokines lymphocytes dendritic cells epithelium 


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Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Arthur Kaser
    • 1
  • Othmar Ludwiczek
    • 1
  • Sandra Holzmann
    • 2
  • Alexander R. Moschen
    • 1
  • Günter Weiss
    • 3
  • Barbara Enrich
    • 3
  • Ivo Graziadei
    • 4
  • Stefan Dunzendorfer
    • 3
  • Christian J. Wiedermann
    • 3
  • Elisabeth Mürzl
    • 4
  • Eveline Grasl
    • 4
  • Zerina Jasarevic
    • 4
  • Nikolaus Romani
    • 2
  • Felix A. Offner
    • 4
  • Herbert Tilg
    • 1
  1. 1.Division of Gastroenterology and HepatologyUniversity Hospital InnsbruckInnsbruckAustria
  2. 2.Department of DermatologyUniversity Hospital InnsbruckInnsbruckAustria
  3. 3.Division of General Internal Medicine, Department of MedicineUniversity Hospital InnsbruckInnsbruckAustria
  4. 4.Division of Gastroenterology and HepatologyUniversity Hospital InnsbruckInnsbruckAustria

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