Journal of Insect Conservation

, Volume 7, Issue 4, pp 215–221 | Cite as

Molecular phylogenetic analysis supports the synonymy of Prodontria modesta (Broun) and Prodontria bicolorata Given (Coleoptera: Scarabaeidae: Melolonthinae)

  • Cor J. Vink
  • Alison M. Evans
  • Craig B. Phillips
  • Trudy C. Murdoch
  • Mike B. Tubbs


The validity of the synonymy of Prodontria modesta (Broun) and the nationally endangered Prodontria bicolorata Given, both flightless with very restricted distributions in Central Otago, New Zealand, was investigated using molecular phylogenetic analyses of a section of the mitochondrial gene region coding for cytochrome oxidase subunit 1 (COI) and nuclear rDNA internal transcribed spacer region (ITS1). Maximum likelihood analysis of the COI data found no separate, monophyletic lineages linked to colour form among six populations of the beetle and the genetic variation observed was less than that recorded within many other beetle species, including other melolonthines. The synonymisation of P. bicolorata with P. modesta should be considered valid and conservation efforts should focus on protecting habitats and populations, rather than on preserving colour forms.

COI ITS1 Molecular phylogenetics Prodontria modesta Synonymy 


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  1. Benson D.A., Karsch-Mizrachi I., Lipman D.J., Ostell J., Rapp B.A. and Wheeler D.L. 2002. GenBank. Nucleic Acids Res. 30: 17-20.Google Scholar
  2. Broun T. 1909. Descriptions of new genera and species of NewZealand Coleoptera. Ann. Mag. Nat. Hist. 8(3): 385-415.Google Scholar
  3. Brower A.V.Z. 1994. Rapid morphological radiation and convergence among races of the butterfly Heliconius erato inferred from patterns of mitochondrial DNA evolution. Proc. Natl Acad. Sci. USA 91: 6491-6495.Google Scholar
  4. Emerson B.C. 1994. Conservation status of chafer beetles Prodontria bicolorata and P. modesta: distribution and ecological observations. NZ Entomol. 17: 3-6.Google Scholar
  5. Emerson B.C. and Barratt B.I.P. 1997. Descriptions of seven new species of the genus Prodontria Broun (Coleoptera: Scarabaeidae: Melolonthinae). Coleop. Bull. 51: 23-36.Google Scholar
  6. Emerson B.C. and Wallis G.P. 1994. Species status and population genetic structure of the flightless chafer beetles Prodontria modesta and P. bicolorata (Coleoptera; Scarabaeidae) from South Island, New Zealand. Mol. Ecol. 3: 339-345.Google Scholar
  7. Emerson B.C. and Wallis G.P. 1995. Phylogenetic relationships of the Prodontria (Coleoptera; Scarabaeidae; subfamily Melolonthinae), derived from sequence variation in the mitochondrial cytochrome oxidase II gene. Mol. Phylogenet. Evol. 4: 433-447.Google Scholar
  8. Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783-791.Google Scholar
  9. Given B.B. 1952. A Revision of the Melolonthinae of New Zealand. Part I: The Adult Beetles. New Zealand Department of Scientific and Industrial Research Bulletin 102, Entomological Research Station Publication 10, Department of Scientific and Industrial Research, Wellington.Google Scholar
  10. Hasegawa M., Kishino K. and Yano T. 1985. Dating the human-ape splitting by a molecular clock of mitochondrial DNA. J. Mol. Evol. 22: 160-174.Google Scholar
  11. Hitchmough R. (compiler) 2002. New Zealand Threat Classification System Lists - 2002. Threatened Species Occasional Publication 23, Department of Conservation, Wellington.Google Scholar
  12. Jamieson C.J. 1999. Conservation Management of Prodontria Species in the Alexandra Area. Unpublished Report, Department of Conservation, Dunedin.Google Scholar
  13. Kelley S.T., Mitton J.B. and Paine T.D. 1999. Strong differentiation in mitochondrial DNA of Dendroctonus brevicomis (Coleoptera: Scolytidae) on different subspecies of ponderosa pine. Ann. Entomol. Soc. Am. 92: 193-197.Google Scholar
  14. Lunt D.H., Zhang D.-X., Szymura J.M. and Hewitt G.M. 1996. The insect cytochrome oxidase I gene: evolutionary patterns and conserved primers for phylogenetic studies. Insect Mol. Biol. 5: 153-165.Google Scholar
  15. McGuiness C.A. 2001. The Conservation Requirements of New Zealand's Nationally Threatened Invertebrates. Threatened Species Occasional Publication No. 20, Department of Conservation, Wellington.Google Scholar
  16. Maddison W.P. and Maddison D.R. 1992. MacClade: Analysis of Phylogeny and Character Evolution, Version 3.03. Sinauer Associates, Sunderland.Google Scholar
  17. Mander C.V., Phillips C.B., Glare T.R. and Chapman R.B. 2003. Preliminary assessment of COI and ITS1 sequence variation in Fuller's rose weevil. NZ Plant Prot. 56: 190-193.Google Scholar
  18. Posada D. and Crandall K.A. 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817-818.Google Scholar
  19. Posada D. and Crandall K.A. 2001. Selecting the best-fit model of nucleotide substitution. Syst. Biol. 50: 580-601.Google Scholar
  20. Richards N.K., Glare T.R. and Hall D.C.A. 1997. Genetic variation in grass grub, Costelytra zealandica, from several regions. Proceedings of the 50th New Zealand Plant Protection Conference, pp. 338-343.Google Scholar
  21. Simon C., Frati F., Beckenbach A., Crespi B., Liu H. and Flook P. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann. Entomol. Soc. Am. 87: 651-701.Google Scholar
  22. Swofford D.L. 2002. PAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods), Version 4.0b10. Sinauer Associates, Sunderland.Google Scholar
  23. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F. and Higgins D.G. 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25: 4876-4882.Google Scholar
  24. Wallis G.P. 2001. Taxonomic status of the chafer beetles Prodontria modesta and P. bicolorata. Department of Conservation Internal Series 10: 1-13.Google Scholar
  25. Watt J.C. 1979. Conservation of the Cromwell chafer Prodontria lewisi (Coleoptera: Scarabaeidae). NZ J. Ecol. 2: 22-29.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Cor J. Vink
    • 1
  • Alison M. Evans
    • 2
  • Craig B. Phillips
    • 3
  • Trudy C. Murdoch
    • 4
  • Mike B. Tubbs
    • 4
  1. 1.Ecology and Entomology GroupLincoln UniversityCanterburyNew Zealand
  2. 2.Department of BiologySan Diego State University, SanDiegoUSA
  3. 3.AgResearchLincoln, CanterburyNew Zealand
  4. 4.Department of ConservationCentral Otago Area OfficeAlexandraNew Zealand

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