Journal of Assisted Reproduction and Genetics

, Volume 21, Issue 8, pp 307–309 | Cite as

Freezing of Human Immature Oocytes Using Cryoloops with Taxol in the Vitrification Solution

  • Kohei Fuchinoue
  • Noritaka Fukunaga
  • Setsuyo Chiba
  • Yukiko Nakajo
  • Akiko Yagi
  • Koichi Kyono
Article

Abstract

Purpose: In human frozen immature oocytes, there has been little successful delivery. We examined the feasibility of vitrification solution including Taxol, cytoskeltal stabilizer.

Methods: We set four experimental groups that immature oocytes has cumulus cells or not, or including Taxol or not in the vitrification solution. Frozen–thawed oocytes have been performed IVM, ICSI, and IVC.

Results: There were no significant differences in survival, maturation, and fertilization rate, respectively. However, in the group enveloped by cumulus cells and including Taxol in the vitrification solution, one embryo was developed to blastocyst.

Conclusions: Our results showed that using vitrification solution with Taxol proved so effective.

Cryoloop immature oocytes Taxol vitrification 

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REFERENCES

  1. 2.
    Park SE, Son WY, Lee SH, Lee KA, Ko JJ, Cha KY: Chromo-some and spindle configurations of human oocytes matured in vitro after cryopreservation at the germinal vesicle stage. Fertil Steril 1997;68:920-926Google Scholar
  2. 2.
    Tucker MJ, Wright G, Morton PC, Massey JB: Birth after cryopreservation of immature oocytes with subsequent in vitro maturation. Fertil Steril 1998;70:578-579Google Scholar
  3. 3.
    Chen C. Pregnancy after human oocyte cryopreservation. Lancet 1986;884-886Google Scholar
  4. 4.
    Porcu E, Fabbri R, Seracchioli R, Ciotti PM, Magrini O, Flamigni C: Birth of a healthy female after intracytoplasmic sperm injection of cryopreserved human oocytes. Fertil Steril 1997;68:724-726Google Scholar
  5. 5.
    Yang D, Winslow KL, Blohm PL: Oocyte donation using cryopreserved donor oocytes. Fertil Steril (Suppl) 2002;76:S14Google Scholar
  6. 6.
    Kyono K, Fukunaga N, Haigo K, Chiba S, Sato T: Pregnancy and delivery of a healthy female infant after intracytoplasmic sperm injection into cryopreserved human oocytes. Jpn J Fertil Steril 2001;46:171-177Google Scholar
  7. 7.
    Kasai M, Komi JH, Takakamo A, Tsudera H, Sakurai T, Machida T: A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fertil 1990;89:91-97Google Scholar
  8. 8.
    Kuwayama M, Hamano S, Nagai T: Vitrification of bovine blas-tocysts obtained by in vitro culture of oocytes matured and fertilized in vitro. J Reprod Fertil 1992;96:187-193Google Scholar
  9. 9.
    Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen H, Greve T, Callesen H: Open pulled straw vitrification: A new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 1998;51:38-58Google Scholar
  10. 10.
    Rall WF and Fathy GM: Ice-free cryopreservation of mouse embryos by vitrification. Nature 1985;313:535-573Google Scholar
  11. 11.
    Cha KY, Hong SW, Chuang HM: Pregnancy and implanta-tion from vitrified oocytes following in vitro fertilization. Fertil Steril (Suppl) 1999;72:S2Google Scholar
  12. 12.
    Kuleshova L, Gianaroli L, Magli C, Ferraretti A, Trounson A: Birth following vitrification of a small number of human oocytes. Hum Reprod 1999:14;3077-3079Google Scholar
  13. 13.
    Yoon TK, Chung HM, Lim JM, Han SY, Ko JJ, Cha KY: Pregnancy and delivery of healthy infants developed from vitrified oocytes in a stimulated in vitro fertilization-embryo transfer program. Fertil Steril 2000;74:180-181Google Scholar
  14. 14.
    Park SE, Chung HM, Cha KY, Hwang WS, Lee ES, Lim JM: Cryopreservation of ICR mouse oocyte: Improved post-thawed preimplantation development after vitrification using Taxol, a cytoskeleton stabilizer. Fertil Steril 2001;75:1177-1184Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • Kohei Fuchinoue
    • 1
  • Noritaka Fukunaga
    • 1
  • Setsuyo Chiba
    • 1
  • Yukiko Nakajo
    • 1
  • Akiko Yagi
    • 1
  • Koichi Kyono
    • 1
  1. 1.Department of Gynecology and UrologyLadies Clinic Kyono. Kyono Reproduction Research CenterUSA

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