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Coculture with a Human Granulosa Cell Line Enhanced the Development of Murine Preimplantation Embryos via SCF/c-Kit System

  • Fuminori TaniguchiEmail author
  • Tasuku Harada
  • Makoto Nara
  • Imari Deura
  • Masahiro Mitsunari
  • Naoki Terakawa
Article

Abstract

Purpose: To evaluate the effect on the coculture of murine embryos with a human ovarian granulosa tumor derived cell line (KGN cells).

Methods: We observed microscopically the growth of murine preimplantation embryos in the coculture system with KGN cells or in the presence with exogenous stem cell factor (SCF). The reverse transcriptase–polymerase chain reaction (RT-PCR) method was used to analyze the gene expression of SCF in KGN cells cocultured with murine embryos.

Results: The coculture system with KGN cells significantly increased the rate of embryo development to late blastocyst and to hatching stage. We also found that coculture with murine embryos enhanced the gene expression of SCF in KGN cells. Adding human recombinant SCF to the medium significantly enhanced embryo development to late blastocyst and hatching stage.

Conclusions: KGN cells may facilitate preimplantion embryo development through SCF/c-kit paracrine system.

Coculture embryo development KGN cells, SCF/c-kit 

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Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • Fuminori Taniguchi
    • 1
    Email author
  • Tasuku Harada
    • 1
  • Makoto Nara
    • 1
  • Imari Deura
    • 1
  • Masahiro Mitsunari
    • 1
  • Naoki Terakawa
    • 1
  1. 1.Department of Obstetrics and GynecologyTottori University School of MedicineYonagoJapan

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