Advertisement

Plant Growth Regulation

, Volume 42, Issue 3, pp 203–211 | Cite as

Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and antioxidative system of rice (Oryza sativa L.) under salinity stress

  • Filiz Özdemir
  • Melike Bor
  • Tijen Demiral
  • İsmail Türkan
Article

Abstract

The effects of 24-epibrassinolide (24-epiBL) on seedling growth, antioxidative system, lipid peroxidation, proline and soluble protein content were investigated in seedlings of the salt-sensitive rice cultivar IR-28. Seedling growth of rice plants was improved by 24-epiBL treatment under salt stress conditions. When seedlings treated with 24-epiBL were subjected to 120 mM NaCl stress, the activities of superoxide dismutase (EC 1.15.1.1), catalase (EC 1.11.1.6) and glutathione reductase (EC 1.6.4.2) did not show significant difference, whereas the activity of ascorbate peroxidase (EC 1.11.1.11) significantly increased. Increased activity of peroxidase (EC 1.11.1.7) under NaCl stress showed remarkable decrease in the 24-epiBL+NaCl-applied group. Lipid peroxidation level significantly increased under salt stress but decreased with 24-epiBL application revealing that less oxidative damage occurred in this group (24-epiBL+NaCl). In addition, increased proline content in the NaCl-applied group was decreased by 24-epiBL application in the 24-epiBL+NaCl-applied group. Soluble protein content was increased by 24-epiBL application even under NaCl stress, being also higher than control conditions (no 24-epiBL or NaCl treatment). 24-epiBL treatment considerably alleviated oxidative damage that occurred under NaCl-stressed conditions and improved seedling growth in part under salt stress in sensitive IR-28 seedlings.

Antioxidant enzymes 24-epibrassinolide Lipid peroxidation Proline Rice (Oryza sativa L.) Salt stress 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Acar O., Türkan I. and Özdemir F. 2001. Superoxide dismutase and peroxidase activities in drought sensitive and resistant barley (Hordeum vulgare L.) varieties. Acta Physiol. Plant. 23(3): 351–356.Google Scholar
  2. Anuradha S. and Rao S.S.R. 2003. Application of brassinosteroids to rice seeds (Oryza sativa L.) reduced the impact of salt stress on growth, prevented photosynthetic pigment loss and increased nitrate reductase activity. Plant Growth Regul. 40: 29–32.CrossRefGoogle Scholar
  3. Aono M., Saji H., Fujiyama K., Sugita M., Kondo N. and Tanaka K. 1995. Decrease in activity of glutathione reductase enhances paraquat sensitivity in transgenic Nicotiana tabacum. Plant Physiol. 107: 645–648.PubMedGoogle Scholar
  4. Bajguz A. 2000a. Effect of brassinosteroids on nucleic acids and protein content in cultured cells of Chlorella vulgaris. Plant Physiol. Biochem. 38(3): 209–215.CrossRefGoogle Scholar
  5. Bajguz A. 2000b. Blockade of heavy metals accumulation in Chlorella vulgariscells by 24-epibrassinolide. Plant Physiol. Biochem. 38: 797–801.Google Scholar
  6. Bates L.S., Waldren R.P. and Teare I.D. 1973. Rapid determination of free proline for water-stress studies. Plant Soil 39: 205–207.CrossRefGoogle Scholar
  7. Beauchamp C.O. and Fridovich I. 1971. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276–287.CrossRefPubMedGoogle Scholar
  8. Bergmeyer N. 1970. Methoden der enzymatischen Analyse, vol. 1, Akademie Verlag, Berlin, pp. 636–647.Google Scholar
  9. Bor M., Özdemir F. and Türkan I. 2003. The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritimaL. Plant Sci. 164: 77–84.CrossRefGoogle Scholar
  10. Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.CrossRefPubMedGoogle Scholar
  11. Clouse S.D., Langford M., Hall A.F., McMorris T.C. and Baker M.E. 1993. Physiological and molecular effects of brassinosteroids on Arabidopsis thaliana. J. Plant Growth Regul. 12: 61–66.CrossRefGoogle Scholar
  12. Dhaubhadel S., Chaudhary S., Dobinson K.F. and Krishna P. 1999. Treatment with 24-epibrassinolide, a brassinosteroid, increases the basic thermotolerance of Brassica napusand tomato seedlings. Plant Mol. Biol. 40: 333–342.CrossRefPubMedGoogle Scholar
  13. Dionisio-Sese M.L. and Tobita S. 1998. Antioxidant responses of rice seedlings to salinity stress. Plant Sci. 135: 1–9.CrossRefGoogle Scholar
  14. Foyer C.H. and Halliwell B. 1976. The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133: 21–25.CrossRefGoogle Scholar
  15. Fujioka S. 1999. Natural occurrence of brassinosteroids in the plant kingdom. In: Sakurai A., Yokota T. and Clouse S.D. (eds), Brassinosteroids: Steroidal Plant Hormones, Springer-Verlag, Tokyo, pp. 21–45.Google Scholar
  16. Fujioka S. and Sakurai A. 1997. Brassinosteroids. Nat. Prod. Rep. 14: 1–10.CrossRefPubMedGoogle Scholar
  17. Grove M.D., Spencer G.F., Rohwedder W.K., Mandava N.B., Worley J.F., Warthen J.D., Steffens G.L., Flippen-Anderson J.L. and Cook J.C. 1979. Brassinolide, a plant growthpromoting steroid isolated from Brassica napuspollen. Nature 281: 216–217.CrossRefGoogle Scholar
  18. Hasegawa P.M., Bressan R.A., Zhu J.K. and Bohnert H.J. 2000. Plant cellular and molecular responses to high salinity. Annu. Rev. Plant Physiol. Plant Mol. Biol. 51: 463–499.CrossRefPubMedGoogle Scholar
  19. Hernández J.A., Jiménez A., Mullineaux P. and Sevilla F. 2000. Tolerance of pea (Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defenses. Plant Cell Environ. 23: 853–862.Google Scholar
  20. Herzog V. and Fahimi H. 1973. Determination of the activity of peroxidase. Anal. Biochem. 55: 554–562.CrossRefPubMedGoogle Scholar
  21. Jain M., Mathur G., Koul S. and Sarin N.B. 2001. Ameliorative effects of proline on salt stress-induced lipid peroxidation in cell lines of groundnut (Arachis hypogea L.). Plant Cell Rep. 20: 463–468.Google Scholar
  22. Li J.M., Nagpal P., Vitart V., Mc Morris T.C. and Chory J. 1996. A role for brassinosteroids in light dependent development of Arabidopsis. Science 272: 398–401.PubMedGoogle Scholar
  23. Li L. and Van Staden J. 1998. Effects of plant growth regulators on drought resistance of two maize cultivars. S. Afr. J. Bot. 64(2): 116–120.Google Scholar
  24. Li L., Van Staden J. and Jäger A.K. 1998. Effects of plant growth regulators on the antioxidant system in seedlings of two maize cultivars subjected to water stress. Plant Growth Reg. 25: 81–87.Google Scholar
  25. Lin J.N. and Kao C.H. 1998. Effect of oxidative stress caused by hydrogen peroxide on senescence of rice leaves. Bot. Bull. Acad. Sin. 39: 161–165.Google Scholar
  26. Madhava Rao K.V. and Sresty T.V.S. 2000. Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan L. Millspaugh) in response to Zn and Ni stresses. Plant Sci. 157: 113–128.CrossRefPubMedGoogle Scholar
  27. Mandava N.B. 1988. Plant growth-promoting brassinosteroids. Annu. Rev. Plant Physiol. Plant Mol. Biol. 39: 23–52.CrossRefGoogle Scholar
  28. McCord J.M. 2000. The evolution of free radicals and oxidative stress. Am. J. Med. 108: 652–659.CrossRefPubMedGoogle Scholar
  29. Mittal R. and Dubey R.S. 1991. Behaviour of peroxidases in rice: changes in enzyme activity and isoforms in relation to salt tolerance. Plant Physiol. Biochem. 29(1): 31–40.Google Scholar
  30. Nakano Y. and Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate-spesific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22(5): 867–880.Google Scholar
  31. Noctor G. and Foyer C.H. 1998. Ascorbate and glutathione: Keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49: 249–279.CrossRefPubMedGoogle Scholar
  32. Nomura T., Nakayama N., Reid J.B., Takeuchi Y. and Yokota T. 1997. Blockage of brassinosteroid biosynthesis and sensitivity cause dwarfism in Pisum sativum. Plant Physiol. 113: 31–37.PubMedGoogle Scholar
  33. Sasse J.M. 1997. Recent progress in brassinosteroid research. Physiol. Plant. 100: 696–701.CrossRefGoogle Scholar
  34. Sasse J.M. 1999. Physiological actions of brassinosteroids. In: Sakurai A., Yokota T. and Clouse S.D. (eds), Brassinosteroids: Steroidal plant hormones, Springer-Verlag, Tokyo, pp. 137–161.Google Scholar
  35. Sasse J.M., Smith R. and Hudson I. 1995. Effects of 24-epibrassinolide on germination of seed of Eucalyptus camaldulensisin saline conditions. Proc. Plant Growth Regul. Soc. Amer. 22: 136–141.Google Scholar
  36. Schumacher K. and Chory J. 2000. Brassinosteroid signal transduction: still casting the actors. Curr. Opin. Plant Biol. 3: 79–84.CrossRefPubMedGoogle Scholar
  37. Singha S. and Choudhuri M.A. 1990. Effect of salinity (NaCl) stress on H2O2 metabolism in Vigna and Oryza seedlings. Biochem. Physiol. Pflanzen 186: 69–74.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Filiz Özdemir
    • 1
  • Melike Bor
    • 1
  • Tijen Demiral
    • 1
  • İsmail Türkan
    • 1
  1. 1.Faculty of Science, Department of BiologyEge UniversityBornova-İzmirTurkey

Personalised recommendations