Genetic Resources and Crop Evolution

, Volume 51, Issue 6, pp 569–575

Protein heterogeneity in European wheat landraces and obsolete cultivars: Additional information

  • Edita Gregová
  • Jiří Hermuth
  • Ján Kraic
  • Ladislav Dotlačil
Article

Abstract

The objective of this study was to determine the composition of high molecular weight glutenin subunits of landraces and obsolete cultivars. Altogether glutenin profiles of 67 European wheats were analyzed by sodiumdodecylsulphate polyacrylamide gel electrophoresis. Nineteen of them were observed to be homogeneous, whereas 48 (71%) were heterogeneous in glutenin profiles. Heterogeneous accessions possessed from 2 to 9 different glutenin phenotypes. Seventeen high molecular weight (HMW)-glutenin subunits have been found, three belonged to Glu-1A, 11 to Glu-1B, and three to Glu-1D locus. The most frequented HMW-GS at the Glu-A1, Glu-B1, and Glu-D1 complex loci were 0, 7+9, and 2+12, respectively. However, allele low frequented in wheat such as 13+16, 20, 6, 7, 8, and 9 were observed also. Furthermore, other new alleles encoding HMW-GS at the locus Glu-1B with relative molecular weight 120 and 104 kDa have been found in one of the line of the Swedish cultivar Kotte. TheGlu-1 quality score in the examined accessions varied broadly with some lines reaching the maximum value of 10.

Glutenins Landraces Obsolete cultivars Storage proteins Triticum aestivum L. Wheat 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Edita Gregová
    • 1
  • Jiří Hermuth
    • 1
  • Ján Kraic
    • 1
  • Ladislav Dotlačil
    • 1
  1. 1.Department of Applied Biochemistry and Molecular BiologyResearch Institute of Plant ProductionPiešt'anySlovak Republic

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