Glycoconjugate Journal

, Volume 20, Issue 2, pp 83–89 | Cite as

Specificity of human anti-carbohydrate IgG antibodies as probed with polyacrylamide-based glycoconjugates

  • E.P. Smorodin
  • O.A. Kurtenkov
  • B.L. Sergeyev
  • G.V. Pazynina
  • N.V. Bovin


The TF, Tn, and SiaTn glycotopes are frequently expressed in cancer-associated mucins. Antibodies to these glycotopes were found in human serum. A set of polyacrylamide (PAA)—based glycoconjugates was applied to the direct and competitive enzyme-linked immunosorbent assays (ELISA) to characterize the specificity of serum IgG antibodies. The anti-TF, -Tn and -SiaTn IgG were affinity purified from serum of cancer patients and characterized using PAA-conjugates and free saccharides. The anti-TF and -Tn antibodies were shown to be specific. The anti-TF IgG bound both Galβ1-3GalNAcα- and Galβ1-3GalNAcβ-PAA, the latter was three-four times more effective inhibitor of antibody binding. The anti-Tn IgG reacted only with GalNAcα-PAA. The anti-SiaTn IgG cross-reacted with Tn-PAA but SiaTn-PAA was five-six times more effective inhibitor in a competitive assay. The IC50 values for PAA-conjugates with the corresponding antibodies typically ranged from 2 to 5 × 10−8 M. The antibodies display a low specificity to mucin-type glycoconjugates in comparison with PAA-conjugates as was shown for mucins isolated from human malignant tumor tissues, ovine submaxillary mucin (OSM) and asialo-OSM. The unusual IgG-antibody specificity to GalNAcβ and GalNAcβ1-3GalNAcβ ligands was found in human serum. Published in 2004.

antibodies glycoconjugates mucins TF Tn SiaTn 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • E.P. Smorodin
    • 1
  • O.A. Kurtenkov
    • 1
  • B.L. Sergeyev
    • 1
  • G.V. Pazynina
    • 2
  • N.V. Bovin
    • 2
  1. 1.Institute of Experimental & Clinical MedicineTallinnEstonia
  2. 2.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryMoscowRussia

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