Glycoconjugate Journal

, Volume 19, Issue 7–9, pp 615–619 | Cite as

The involvement of galectin-1 in skeletal muscle determination, differentiation and regeneration

  • Diana J. Watt
  • Gareth E. Jones
  • Kirstin Goldring


The dogma that a cell is rigidly committed to one tissue type has been heavily challenged over the past few years with numerous reports of transdifferentiation of cells between different lineages. Cells capable of entering lineages other than that of their tissue of origin have been identified in several diverse tissues. Recently we have focussed on a non-committed myogenic cell within the dermis that is capable, under certain conditions, of expressing muscle specific markers and even fusing to the terminally differentiated stage of muscle cell development. We have identified galectin-1 as being a potent factor implicated in this process. In this review we discuss our findings and consider the involvement of galectin-1 in muscle determination, differentiation and regeneration. Published in 2004.

galectin-1 muscle development dermal fibroblasts 


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  1. 1.
    Gibson AJ, Karasinski J, Relvas J, Moss J, Sherratt TG, Strong PN, Watt DJ, Dermal fibroblasts convert to a myogenic lineage in Timdx mouse muscle, J Cell Sci 108, 207-14 (1995).Google Scholar
  2. 2.
    Wise CJ, Watt DJ, Jones GE, Conversion of dermal fibroblasts to a myogenic lineage is induced by a soluble factor derived from myoblasts, J Cell Biochem 61, 363-74 (1996).Google Scholar
  3. 3.
    Goldring K, Jones GE, Watt DJ, A factor implicated in the myogenic conversion of non-muscle cells derived from the mouse dermis, Cell Transpl 9, 519-29 (2000).Google Scholar
  4. 4.
    Goldring K, Jones GE, Thiagarajah R, Watt DJ, In vitro effects of galectin-1 on the differentiation of myoblasts and fibroblasts, J Cell Sci 115, 355-66 (2002).Google Scholar
  5. 5.
    Goldring K, Jones GE, Sewry CA, Watt DJ, The role of galectin-1 in the conversion of human dermal fibroblasts to a myogenic lineage, Neuromusc Disorders 12, 183-6 (2002).Google Scholar
  6. 6.
    Breton M, Li ZL, Paulin D, Harris JA, Rieger F, Pincon-Raymond M, Garcia L, Myotube driven myogenic recruitment of cells during in vitro myogenesis, Dev Dyn 202, 126-36 (1995).Google Scholar
  7. 7.
    Poirier F, Timmons PM, Chan CT, Guenet JL, Rigby PW, Expression of the L14 lectin during mouse embryogenesis suggests multiple roles during pre-and post-implantation development, Development 115, 143-55 (1992).Google Scholar
  8. 8.
    Cooper DNW, Barondes SH, Evidence for export of a muscle lectin from cytosol to extracellular matrix and for a novel secretory mechanism, J Cell Biol 110, 1681-91 (1990).Google Scholar
  9. 9.
    Harrison FL, Wilson TJG, The 14 kDa β-galactoside binding lectin in myoblast and myotube cultures: Localization by confocal microsopy, J Cell Sci 101, 635-46 (1992).Google Scholar
  10. 10.
    Cooper DNW, Massa SM, Barondes SH, Endogenous muscle lectin inhibits myoblast adhesion to laminin, J Cell Biol 115, 1437-48 (1991).Google Scholar
  11. 11.
    Den H, Malinzak DA, Isolation and properties of b-D-galactoside specific lectin from chick embryo thigh muscles, J Biol Chem 252, 5444-8 (1977).Google Scholar
  12. 12.
    Gartner TK, Podleski TR, Evidence that a membrane bound lectin mediates fusion of L6 myoblasts, Biochem Biophys Res Commun 67, 972-8 (1975).Google Scholar
  13. 13.
    Gu M, Wang W, Song WK, Cooper DN, Kaufman SJ, Selective modulation of the interaction of alpha 7 beta 1 integrin with fi-bronectin and laminin by L-14 lectin during skeletal muscle differentiation, J Cell Sci 107, 175-81 (1994).Google Scholar
  14. 14.
    Nowak TP, Haywood PL, Barondes SH, Developmentally regulated lectin in embryonic chick muscle and a myogenic cell line, Biochem Biophys Res Commun 68, 650-57 (1976).Google Scholar
  15. 15.
    Sanford GL, Harris-Hooker S, Stimulation of vascular cell proliferation by beta-galactoside specific lectins, FASEB J 4, 2912-8 (1990).Google Scholar
  16. 16.
    Wells V, Malluchi L, Molecular expression of the negative growth factor murine b-galactoside binding protein (m bGBP), Biochim Biophys Acta 1121, 239-44 (1992).Google Scholar
  17. 17.
    MacBride RG, Przybylski RJ, Purified lectin from skeletal muscle inhibits myotube formation in vitro, J Cell Biol 85, 617-25 (1980).Google Scholar
  18. 18.
    Poirier F, Robertson EJ, Normal development of mice carrying a null mutation in the gene encoding the L14 S-type lectin, Development 119, 1229-36 (1993).Google Scholar
  19. 19.
    Goldring K, Jones G, Poirier F, Prasad R, Tavsandglu Y, Watt D, The galectin-1 null mouse furthers the understanding of the role of galectin-1 in muscle formation, differentiation and regeneration. Abstract of World Muscle Society Conference, Rotterdam Neuromusc Disorders 12, 719-20 (2002).Google Scholar
  20. 20.
    Caldwell CJ, Mattey DL, Weller RO, Role of the basement membrane in the regeneration of skeletal muscle, Neuropathol Appl Neurobiol 16, 225-38 (1990).Google Scholar
  21. 21.
    Megeney LA, Kablar B, Garrett K, Anderson JE, Rudnicki MA, Myo D is required for myogenic stem cell function in adult skeletal muscle, Genes Dev 10, 1173-83 (1996).Google Scholar
  22. 22.
    White JD, Scaffidi A, Davies M, McGeachie J, Rudnicki MA, Grounds MD, Myotube formation is delayed but not prevented in MyoD-deficient skeletal muscle: Studies in regenerating whole muscle grafts of adult mice, J Histochem Cytochem 48, 1531-44 (2000).Google Scholar
  23. 23.
    Brand-Saberi B, Christ B, Evolution and development of distinct cell lineages derived from somites, Curr Top Dev Biol 48, 1-42 (2000).Google Scholar
  24. 24.
    Baum LG, Pang M, Perillo NL, Wu T, Delegeane A, Uittenbogaart CH, Fukuda M, Seilhamer JJ, Human thymic epithelial cells express an endogenous lectin, galectin-1, which binds to core 2 Oglycans on thymocytes and T lymphoblastoid cells, J Exp Med 81, 877-87 (1995).Google Scholar
  25. 25.
    Rabinovich GA, Ariel A, Hershkoviz R, Hirabayashi J, Kasai KI, Lider O, Specific inhibition of T-cell adhesion to extracellular matrix and proinflammatory cytokine secretion by human recombinant galectin-1, Immunology 97, 100-6 (1999).Google Scholar
  26. 26.
    Van den Brule FA, Buicu C, Baldet M, Sobel ME, Cooper DN, Marschal P, Castronovo V, Galectin-1 modulates human melanoma cell adhesion to laminin, Biochem Biophys Res Commun 209, 760-67 (1995).Google Scholar
  27. 27.
    Yamaoka K, Ohno S, Kawasaki H, Suzuki K, Overexpression of a beta-galactoside binding protein causes transformation of BALB3T3 fibroblast cells, Biochem Biophys Res Commun 179, 272-9 (1991).Google Scholar
  28. 28.
    Allen HJ, Sucato D, Woynarowska B, Gottstine S, Sharma A, Bernacki RJ, Role of galaptin in ovarian carcinoma adhesion to extracellular matrix in vitro, J Cell Biochem 43, 43-57 (1990).Google Scholar
  29. 29.
    Adams L, Scott GK, Weinberg CS, Biphasic modulation of cell growth by recombinant human galectin-1, Biochem et Biophys Acta 1312, 137-44 (1996).Google Scholar
  30. 30.
    Do KY, Smith DF, Cummings RD, LAMP-1 in CHO cells is a primary carrier of poly-N-acetyllactosamine chains and is bound preferentially by a mammalian S-type lectin, Biochem Biophys Res Commun 173, 1123-8 (1990).Google Scholar
  31. 31.
    Ozeki Y, Matsui T, Yamamoto Y, Funahashi M, Hamako J, Titani K, Tissue fibronectin is an endogenous ligand for galectin-1, Glycobiology 5, 255-61 (1995).Google Scholar
  32. 32.
    Pace KE, Baum LG, Identification and characterisation of T cell surface counter-receptors for galectin-1, Glycobiol 6, 745 (abstract) (1996).Google Scholar
  33. 33.
    Zhou Q, Cummings RD, The S-type lectin from calf heart tissue binds selectively to the carbohydrate chains of laminin, Arch Biochem Biophys 281, 27-35 (1990).Google Scholar
  34. 34.
    Chung CD, Patel VP, Moran M, Lewis LA, Carrie Miceli M, Galectin-1 induces partial TCR zeta-chain phosphorylation and antagonizes processive TCR signal transduction, J Immunol 165, 3722-9 (2000).Google Scholar
  35. 35.
    Rabinovich GA, Alonso CR, Sotomayor CE, Durand S, Bocco JL, Riera CM, Molecular mechanisms implicated in galectin-1-induced apoptosis: Activation of the AP-1 transcription factor and downregulation of Bcl-2, Cell Death Differ 7, 747-53 (2000).Google Scholar
  36. 36.
    Vespa GN, Lewis LA, Kozak KR, Moran M, Nguyen JT, Baum LG, Miceli MC, Galectin-1 specifically modulates TCR signals to enhance TCR apoptosis but inhibit IL-2 production and proliferation, J Immunol 162, 799-806 (1999).Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Diana J. Watt
    • 1
  • Gareth E. Jones
    • 2
  • Kirstin Goldring
    • 3
  1. 1.Department of AnatomyBrighton and Sussex Medical SchoolBrighton, East SussexUK
  2. 2.Randall Centre for Molecular Mechanisms of Cell FunctionKing's College LondonLondonUK
  3. 3.UK Parkinson's Disease Society Tissue Bank at Imperial College, Division of Neuroscience & Psychological MedicineImperial College Faculty of MedicineLondonUK

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