Fish Physiology and Biochemistry

, Volume 28, Issue 1–4, pp 309–311 | Cite as

Specific gene expression profiles are associated with follicular maturational competence acquisition in rainbow trout (Oncorhynchus mykiss)

  • Julien Bobe
  • Gersende Maugars
  • Thaovi Nguyen
  • Bernard Jalabert
Article

Abstract

The expression profiles of several mRNAs were characterized during follicular maturational competence (FMC) acquisition. Post-vitellogenic female rainbow trout (Oncorhynchus mykiss) were assayed in vitro for follicular maturational competence (FMC). Ovarian follicles were stimulated in vitro for 60 h at 12 °C with a range of concentrations of partially purified gonadotropin and the efficient concentration for 50% germinal vesicle breakdown was calculated and used as an indicator of follicular maturational competence. Before in vitro assay, ovarian tissue was sampled in order to quantify mRNA abundance in the ovarian follicle by real-time PCR. The mRNA expression of Luteinizing Hormone receptor (LH-r), Follicular Stimulating Hormone receptor (FSH-r), Insulin-like Growth Factor 1 (IGF1), Insulin-like Growth Factor 2 (IGF2), Insulin-like Growth Factor receptor 1a (IGF-r1a) and 20β-hydroxysteroid dehydrogenase (20β-HSD), that are putatively expressed in the preovulatory ovary, was thus studied in females of varying FMC.

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References

  1. Jalabert B. 1976. In vitro oocyte maturation and ovulation in rainbow trout (Salmo gairdneri), northern pike (Esox lucius), and goldfish (Carassius auratus). J. Fish. Res. Bd. Can. 33: 974–988.Google Scholar
  2. Jalabert B. 1978. Production of fertilizable oocytes from follicles of rainbow trout (Salmo gairdneri) following in vitro maturation and ovulation. Ann. Biol. Anim. Bioch. Biophys. 18: 461–470.Google Scholar
  3. Jalabert B, Fostier A. 1984. The follicular sensitivity in vitro to maturation-inducing hormones in rainbow trout, Salmo gairdneri: role of oestradiol-17β. Aquaculture 43: 1–11.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Julien Bobe
    • 1
  • Gersende Maugars
    • 1
  • Thaovi Nguyen
    • 1
  • Bernard Jalabert
    • 1
  1. 1.Institut National de la Recherche Agronomique (INRA)SCRIBE.Rennes CedexFrance

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