, Volume 137, Issue 3, pp 291–296

QTL mapping of net blotch resistance genes in a doubled-haploid population of six-rowed barley

  • ZhengQiang Ma
  • Nora L.V. Lapitan
  • Brian Steffenson


Net blotch, caused by Pyrenophora teres f. teres, is a damaging foliar disease of barley worldwide. It is important to identify resistance germplasm and study their genetics. 'Chevron', a six-rowed barley used as a parent for the production of a doubled haploid (DH) population for mapping of Fusarium head blight (FHB) resistance, was also found to be resistant to net blotch. To map the resistance genes, the population was evaluated for resistance at the seedling stage in a greenhouse. The resistance data showed a two-peak distribution. Through linkage mapping, one resistance gene, tentatively called Rpt, was located on chromosome 6HS flanked by Xksua3b-Xwg719d, which was also detected by QTL mapping. This QTL explained 64% of the phenotypic variance for the resistance in this DH population. In addition, a minor QTL was found on chromosome 2HS defined by Xcdo786-Xabc156a. 'Chevron' and 'Stander' contributed the resistant alleles of Rpt and the 2HS QTL, respectively. Both QTLs together explained nearly 70% of the phenotypic variance. The markers for these QTLs are useful for marker-assisted selection of net blotch resistance in barley breeding.

barley mapping net blotch resistance RFLP QTL 


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  1. Chen, F.Q. & P.M. Hayes, 1989. A comparison of Hordeum bulbosum-mediated haploid production efficiency in barley using in vitro floret and tiller culture. Theor Appl Genet 77: 701–704.Google Scholar
  2. Douiyssi, A., D.C. Rasmusson & A.P. Roelfs, 1998. Responses of barley cultivars and lines to isolates of Pyrenophora teres. Plant Dis 82: 316–321.Google Scholar
  3. Jana, S. & K.L. Bailey, 1995. Responses of wild and cultivated barley from west Asia to net blotch and spot blotch. Crop Sci 35: 242–246.Google Scholar
  4. Jonsson, R., T. Bryngelsson & M. Gustafsson, 1997. Virulence studies of Swedish net blotch isolates (Drechslera teres) and identification of resistant barley lines. Euphytica 94: 209–218.Google Scholar
  5. Jonsson, R., T. Sall, T. Kraft & M. Gustafsson, 1999. Inheritance of resistance to Pyrenophora teres f. teres in spring barley. Plant Breed 118: 313–317.Google Scholar
  6. Lander, E.S. & D. Botstein, 1989. Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121: 185–199.Google Scholar
  7. Langridge, P., A. Karakousis, N. Collins, J. Kretschmer & S. Manning, 1995. A consensus linkage map of barley. Mol Breed 1: 389–395.Google Scholar
  8. Ma, Z.Q., B. Steffenson, E. Frost, D. Rasmusson & N.L.V. Lapitan, 2000. Quantitative trait loci mapping for Fusarium head blight resistance genes in six-rowed barley. Phytopath 90: 1079–1088.Google Scholar
  9. Manninen, O., R. Kalendar, J. Robinson & A.H. Schulman, 2000. Application of BARE-1 retrotransposon markers to the mapping of a major resistance gene for net blotch in barley. Mol Gen Genet 264: 325–334.Google Scholar
  10. Molnar, S.J., L.E. James & K.J. Kasha, 2000. Inheritance and RAPD tagging of multiple genes for resistance to net blotch in barley. Genome 43: 224–231.Google Scholar
  11. Richter, K., J. Schondelmaier & C. Jung, 1998. Mapping of quantitative trait loci affecting Drechslera teres resistance in barley with molecular markers. Theor Appl Genet 97: 1225–1234.Google Scholar
  12. Rasmusson, D.C., R.D. Wilcoxson & J.V. Wiersma, 1993. Registration of Stander barley. Crop Sci 33: 1403.Google Scholar
  13. Spaner, D., L.P. Shugar, T.M. Choo, I. Falak, K.G. Briggs, W.G. Legge, D.E. Falk, S.E. Ullrich, N.A. Tinker & B.J. Steffenson, 1997. Mapping of disease resistance loci in barley on the basis of visual assessment of naturally occurring symptoms. Crop Sci 38: 843–850.Google Scholar
  14. Steffenson, B.J. & R.K. Webster, 1992a. Pathotype diversity of Pyrenophora teres f.teres on barley. Phytopath 82: 170–177.Google Scholar
  15. Steffenson, B.J. & R.K. Webster, 1992b. Quantitative resistance to Pyrenophora teres f. teres in barley. Phytopath 82: 407–411.Google Scholar
  16. Steffenson, B.J., P.M. Hayes & A. Kleinhofs, 1996. Genetics of seedling and adult plant resistance to net blotch (Pyrenophora teres f. teres) and spot blotch (Cochliobolus sativus)inbarley. Theor Appl Genet 92: 552–558.Google Scholar
  17. Tekauz, A. 1985. A numerical scale to classify reactions of barley to Pyrenophora teres. Can J Plant Pathol 7: 181–183.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • ZhengQiang Ma
    • 1
    • 2
  • Nora L.V. Lapitan
    • 1
  • Brian Steffenson
    • 1
  1. 1.Department of Soil and Crop SciencesColorado State University, Ft. Collins, COU.S.A.
  2. 2.National Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjing, Jiangsu

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