Environmental Monitoring and Assessment

, Volume 92, Issue 1–3, pp 253–272

A Yeast DNA Microarray for the Evaluation of Toxicity in Environmental Water Containing Burned Ash

  • Hyun J. Kim
  • E. Ishidou
  • E. Kitagawa
  • Y. Momose
  • H. Iwahashi
Article

Abstract

Numerous studies on the hazard assessment and epidemiological healthresponses to burned ash have been reported. However, there is littleinformation on the potential toxicity of unknown chemical complexesin burned ash. For an overall evaluation of the multiple toxicitiesof burned ash, a DNA microarray was used in this study, as a new attempt to assess these toxicities. Using the global gene expression on yeast DNA chip to reflect the changes in mRNA levels, our study discovered a lot of evidences for the action of cell homeostasis and stress response etc., against the toxic effects on yeast cells. On the genes of 5,117 open reading frames(ORFs), as valid spots in a microarray, 997 were up-regulated, 1,259 were down-regulated and 2,861 remained unchanged. A detailed analysis of the microarray revealed the genes that were dynamically correlated to the function of the subcellular localization, energy/metabolism, various stress responses/cell homeostasis and detoxification. Significantly, the toxicities, caused by reactive oxygen species(ROS), metals and the other xenobiotics, were indicated in burned ash. Also, the possibility of mutagenicity of the burned ash was suggested on the basis of the DNA repair related genes.

ash biomarker DNA microarray environmental monitoring toxicity assessment 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Behnisch, P. A., Hosoe, K., Shiozaki, K., Ozaki, H., Nakamura, K. and Sakai S.: 2002, 'Lowtemperature thermal decomposition of dioxin-like compounds in fly ash: combination of chemical analysis with in vitro bioassays (EROD and DR-CALUX)', Environ. Sci. Technol. 36(23), 5211–5217.PubMedGoogle Scholar
  2. Choi, J. H., Lou, W. and Vancura A.: 1998, 'A novel membrane-bound glutathione S-transferase functions in the stationary phase of the yeast Saccharomyces cerevisiae', J. Biol. Chem. 273(45), 29915–29922.PubMedGoogle Scholar
  3. De Virgilio, C., Hottiger, T., Dominguez, J., Boller, T. and Wiemken, A., 1994, 'The role of trehalose synthesis for the acquisition of thermotolerance in yeast', Eur. J. Biochem. 219, 179–186.PubMedGoogle Scholar
  4. Delaunay, A., Isnard, A. D. and Toledano, M.: 2000, 'H2O2 sensing through oxidation of the Yap1 transcription factor', EMBO J. 19, 5157–5166.PubMedGoogle Scholar
  5. Diabate, S., Mulhopt, S., Paur, H. R., Wottrich, R. and Krug, H. F.: 2002, 'In vitro effects of incinerator fly ash on pulmonary macrophages and epithelial cells', Int. J. Hyg. Environ. Health 204(5-6), 323-326Google Scholar
  6. Eisen, M. B., Spellman, P. T., Brown, P. O. and Botstein D.: 1998, 'Cluster analysis and display of genome-wide expression patterns', Proc. Natl. Acad. Sci. 95(25), 14863–14868PubMedGoogle Scholar
  7. Fujita, K., Iwahashi, H., Kawai, R. and Komatsu, Y.: 1998, 'Hsp 104 expression and morphological changes associated with disinfectants in environmental bioassay using stress response', Water Sci. Technol. 38, 237–243.Google Scholar
  8. Guthrie, C.: 1990, 'Guide to Yeast Genetics and Molecular Biology', in G. R. Funk (ed.), Methods in Enzymology, Academic, San Diego, CA, U.S.A., pp. 398–401Google Scholar
  9. Hohmann, S.: 2002, 'Osmotic stress signaling and osmoadaptation in yeast', Microbiol. Mol. Biol. Rev. 66(2), 300–372.PubMedGoogle Scholar
  10. Ishikawa, T.: 1992, 'The ATP-dependent glutathione S-conjugate export pump', Trends Biochem. Sci. 17(11), 463–468.PubMedGoogle Scholar
  11. Jensen, L. T., Howard, W. R., Strain, J. J., Winge, D. R. and Culotta, V. C.: 1996, 'Enhanced effectiveness of copper ion buffering by CUP1 metallothionein compared with CRS5 metallothionein in Saccharomyces cerevisiae', J. Biol. Chem. 271(31), 18514–18519.PubMedGoogle Scholar
  12. Kille, P., Hemmings, A. and Lunney, E. A.: 1994, 'Memories of metallothionein',Biochim. Biophys. Acta 1205(2), 151–161PubMedGoogle Scholar
  13. Kitagawa, E., Takahashi, J., Momose, Y. and Iwahashi, H.: 2002, 'Effects of the pesticide thiuram: genome-wide screening of indicator genes by yeast DNA microarray', Environ. Sci. Technol. 36(18), 3908–3915.PubMedGoogle Scholar
  14. Koerkamp, M. G., Rep, M. and Bussemaker, H. J. et al.: 2002, 'Dissection of transient oxidative stress response in Saccharomyces cerevisiae by using DNA microarrays', Mol. Biol. Cell. 13(8), 2783–2794.PubMedGoogle Scholar
  15. Lesuisse, E. and Labbe, P.: 1995, 'Effects of cadmium and of YAP1 and CAD1/YAP2 genes on iron metabolism in the yeast Saccharomyces cerevisiae', Microbiology 141(Pt 11), 2937–2943.PubMedGoogle Scholar
  16. Mager, W. H. and Moradas-Ferreira, P.: 1993, 'Stress response of the yeast', Biochem. J. 290, 1–13.PubMedGoogle Scholar
  17. Mager, W. H. and Varela, J. C. S.: 1993, 'Osmostress response of the yeast Saccharomyces cerevisiae', Mol. Microbiol. 10, 253–258.PubMedGoogle Scholar
  18. McClellan, R. O.: 2002, 'Setting ambient air quality standards for particulate matter', Toxicol. 27(181-182), 329–347.Google Scholar
  19. Momose, Y. and Iwahashi, H.: 2001, 'Bioassay of cadmium using a DNA microarray genome-wide expression patterns of Saccharomyces cerevisiae response to cadmium', Environ. Toxicol. Chem. 20(10), 2353–2360.PubMedGoogle Scholar
  20. Moradas-Ferreira, P., Costa, V., Piper, P. and Mager, W.: 1996, 'The molecular defenses against reactive oxygen species in yeast', Mol. Microbiol. 19(4), 651–658.PubMedGoogle Scholar
  21. Nakajima-Shimada, J., Sakaguchi, S., Tsuji, F. I., Anraku, Y. and Iida, H.: 2000, 'Ca2+ signal is generated only once in the mating pheromone response pathway in Saccharomyces cerevisiae', Cell. Struct. Funct. 25(2), 125–131.PubMedGoogle Scholar
  22. Prahalad, A. K., Inmon, J., Dailey, L. A., Madden, M. C., Ghio, A. J. and Gallagher, J. E.: 2001, 'Air pollution particles mediated oxidative DNA base damage in a cell free system and in human airway epithelial cells in relation to particulate metal content and bioreactivity', Chem. Res. Toxicol. 14(7), 879–887.PubMedGoogle Scholar
  23. Quay, J. L., Reed, W., Samet, J. and Devlin, R. B.: 1998, 'Air pollution particles induce IL-6 gene expression in human airway epithelial cells via NF-kappaB activation', Am. J. Respir. Cell. Mol. Biol. 19(1), 98–106.PubMedGoogle Scholar
  24. Silbajoris, R., Ghio, A. J., Samet, J. M., Jaskot, R., Dreher, K. L. and Brighton, L. E.: 2000, 'In vivo and in vitro correlation of pulmonary MAP kinase activation following metallic exposure', Inhal. Toxicol. 12(6), 453–468.PubMedGoogle Scholar
  25. Varela, J. C., Praekelt, U. M., Meacock, P. A., Planta, R. J. and Mager, W. H.: 1995, 'The Saccharomyces cerevisiae HSP12 gene is activated by the high-osmolarity glycerol pathway and negatively regulated by protein kinase A', Mol. Cell. Biol. 15(11), 6232–6245.PubMedGoogle Scholar
  26. Vido, K., Spector, D., Lagniel, G., Lopez, S. and Toledano, M.: 2001, 'A proteome analysis of the cadmium response in Saccharomyces cerevisiae', J. Biol. Chem. 276, 8469–8474.PubMedGoogle Scholar
  27. Volkert, M. R., Elliott, N. A. and Housman, D. E.: 2000, 'Functional genomics reveals a family of eukaryotic oxidation protection genes', Proc. Natl. Acad. Sci. 97(26), 14530–14535.PubMedGoogle Scholar
  28. Werner, M., Braun, E., Johnston, G. C. and Singer, R. A.: 1993, 'Stationary phase in the yeast Saccharomyces cerevisiae', Microbiol. Rev. 57, 383–401.PubMedGoogle Scholar
  29. Zahringer, H., Holzer, H. and Nwaka, S.: 1998, 'Stability of neutral trehalose during heat stress in Saccharomyces cerevisiae is dependent on the activity of the catalytic subunits of campdependent protein kinase Tpk2 and Tpk2', Eur. J. Biochem. 255, 544–551.PubMedGoogle Scholar
  30. Zuk, D., Belk, J. P. and Jacobson A., 1999, 'Temperature-sensitive mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover', Genetics 153(1), 35–47.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Hyun J. Kim
  • E. Ishidou
  • E. Kitagawa
  • Y. Momose
  • H. Iwahashi

There are no affiliations available

Personalised recommendations