European Journal of Plant Pathology

, Volume 110, Issue 7, pp 735–746

Effect of Trichothecenes Produced by Fusarium graminearum during Fusarium Head Blight Development in Six Cereal Species

  • François Langevin
  • François Eudes
  • André Comeau
Article
  • 349 Downloads

Abstract

Fusarium head blight (FHB) is a complex cereal disease associated with trichothecene production; these mycotoxins are factors of aggressiveness in wheat. Six species (bread and durum wheat, triticale, rye, barley and oats) were submitted to point inoculations with two isogenic strains of Fusarium graminearum; a wild strain (Tri5 +) produced trichothecenes and the mutated strain (Tri5 −) did not. The trichothecene-producing strain was generally more aggressive than the non-producing strain, but this varied according to crop species. The difference in aggressiveness was less pronounced in rye, a very resistant species. High resistance levels were observed in oats due to the large spacing between florets. In six-row barley, despite the existence of a moderate Type II resistance, the fungus was often observed to move externally from one floret to another within the dense spike, without penetrating the rachis. Bread wheat had low resistance to the trichothecene-producing strain and good resistance to the non-producing strain. Triticale responded to the strains in a similar way but was somewhat more resistant to both: symptoms on the spikelets and rachis of the triticales were restricted to below the point of inoculation. Durum wheat was susceptible to the trichothecene-producing strain and only moderately resistant to the non-producing strain, which was able to cause serious damage only to this species. Our study confirmed that the role of trichothecenes in FHB pathogenesis differs among species. The failure of the trichothecene non-producing F. graminearum strain to spread within the inflorescence of wheat, triticale, rye and barley, and the significant reduction of spread in the durum wheat spike strongly suggested that trichothecenes are a major determinant of fungal spread and disease development in Triticeae.

barley deoxynivalenol oats resistance rye triticale wheat 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anderson JA, Stack RW, Liu S, Waldron BL, Fjeld AD, Coyne C, Moreno-Sevilla B, Fetch JM, Song QJ, Cregan PB and Frohberg RC (2001)DNA markers for Fusarium head blight resistance QTLs in two wheat populations. Theoretical and Applied Genetics 102:1164–1168.Google Scholar
  2. Arseniuk E, Goral T and Czembor HC (1993)Reaction of triticale, wheat and rye accessions to graminaceous Fusarium spp. infection at the seedling and adult plant growth stages. Euphytica 70:175–183.Google Scholar
  3. Atanassov Z, Nakamura C, Mori N, Kaneda C, Kato H, Jin YZ, Yoshizawa T, Murai K (1994)Mycotoxin production and pathogenicity of Fusarium species and wheat resistance to Fusarium head blight. Canadian Journal of Botany 72: 161–167.Google Scholar
  4. Bai GH, Desjardins AE and Plattner RD (2001)Deoxynivale-nol-non-producing Fusarium graminearum causes initial infection, but does not cause disease spread in wheat spikes. Mycopathologia 153:91–98.Google Scholar
  5. Bai GH and Shaner G (1994)Scab of Wheat: Prospects for Control. Plant Disease 78:760–766.Google Scholar
  6. Bai GH and Shaner G (1996)Variation in Fusarium graminearum and cultivar resistance to wheat scab. Plant Disease 80: 975–979.Google Scholar
  7. Ban T (2000)Review-Studies on the genetics of resistance to Fusarium head blight caused by Fusarium graminearum in wheat. In: Proceeding of the International Symposium on Wheat Improvement for Scab Resistance. May 5–10 (pp 82–93)Suzhou and Nanjing, China.Google Scholar
  8. Cappellini RA and Peterson JL (1965)Macroconidium formation in submerged culture by a nonsporulating strain of Gibberella zeae. Mycologia 57:962–966.Google Scholar
  9. Clear RM and Patrick SK (2000)Fusarium head blight pathogens isolated from Fusarium-damaged kernels of wheat in Western Canada,1993–1998.Canadian Journal of Plant Pathology 22:51–60.Google Scholar
  10. Couture L (1982)Receptivity of spring wheat cereal cultivars to contamination of grain in the in florescence by Fusarium spp. Canadian Journal of Plant Science 62:29–34.Google Scholar
  11. Dahleen LS and McCormick SP (2001)Trichothecene toxin effects on barley callus and seedling growth. Cereal Research Communications 29:115–120.Google Scholar
  12. Desjardins AE, Proctor RH, Bai GH, McCormick SP, Shaner G, Buechley G and Hohn TM (1996)Reduced virulence of trichothecene non-producing mutants of Gibberella zeae in wheat eld tests. Molecular Plant –Microbe Interactions 9: 775–781.Google Scholar
  13. Doohan FM, Mentewab A, Nicholson P (2000) Antifungal activity toward Fusarium culmorum in soluble wheat extracts. Phytopathology 90:666–671.Google Scholar
  14. Eudes F, Comeau A, Rioux S and Collin J (2000)Phytotoxicité de huit mycotoxines associées á la fusariose de l'épi chez le blé.Canadian Journal of Plant Pathology 22:286–292.Google Scholar
  15. Eudes F, Comeau A, Rioux S and Collin J (2001)Impact of trichothecenes on Fusarium head blight (Fusarium graminearum)development in spring wheat (Triticum aestivum ). Canadian Journal of Plant Pathology 23:318–322.Google Scholar
  16. Fedak G, Armstrong KC, Sinha RC, Gilbert J, Procunier JD, Miller D and Pandeya R (1997)Wide crosses to improve Fusarium Blight resistance in wheat. Cereal Research Communication 25:651–654.Google Scholar
  17. Gilbert J, Procunier JD and Aung T (2000)Influence of the D genome in conferring resistance to fusarium head blight in spring wheat. Euphytica 114:181–186.Google Scholar
  18. Harris LJ, Desjardins AE, Plattner RD, Nicholson P, Butler G, Young JC, Weston G, Proctor RH and Hohn TM (1999) Possible role of trichothecene mycotoxins in virulence of Fusarium graminearum on maize. Plant Disease 83:954–960.Google Scholar
  19. Hart LP, Pestka JJ and Liu MT (1984)Effect of kernel development and wet periods on production of deoxynivalenol in wheat infected with Gibberella zeae. Phytopathology 74:1415–1418.Google Scholar
  20. Hidy PH, Hodge EB, Urry WH and Wehrmeister HL (1966) The structure of zearalenone. Tetrahedron Letters 27:3109–3114.Google Scholar
  21. Hohn TM (1997)Fungal phytotoxins: Biosynthesis and activity. In: Carroll GC and Tudzynski P (eds)The Mycota V Part A: Plant Relationships (pp 129–144)Springer-Verlag, Berlin, Heidelberg.Google Scholar
  22. Lemmens M, Josephs R, Schuhmacher R, Grausgruber H, Buerstmayr H, Ruckenbauer P, Neuhold G, Fidesser M and Krska R (1997)Head blight (Fusarium spp.)on wheat: Investigations on the relationship between disease symptoms and mycotoxin content. Cereal Research Communications 25:459–465.Google Scholar
  23. Ma Z, Steffenson BJ, Prom LK and Lapitan NLV (2000) Mapping of Quantitative Trait Loci for Fusarium Head Blight resistance in barley. Phytopathology 90:1079–1088.Google Scholar
  24. McCallum B, Tekauz A, Gilbert J, Mueller E, Kaethler R, Stulzer M and Kromer U (1999)Fusarium head blight of barley in Manitoba in 1998. Canadian Plant Disease Survey 79:84–85.Google Scholar
  25. McCallum B, Tekauz A and Gilbert J (2001) Vegetative compatibility among Fusarium graminearum (Gibberella zeae) isolates from barley spikes in southern Manitoba. Canadian Journal of Plant Pathology 23:83–87.Google Scholar
  26. McMullen M, Jones R and Gallenberg D (1997)Scab of wheat and barley: A re-emerging disease of devastating impact. Plant Disease 81:1340–1348.Google Scholar
  27. Mesterházy Á(1989)Progress in breeding of wheat and corn genotypes not susceptible to infection by Fusaria. In: Chelkowski J (ed)Fusarium Mycotoxins, Taxonomy and Pathogenicity (pp 357–386)Elsevier, Amsterdam, The Netherlands.Google Scholar
  28. Mesterházy Á(1995) Types and components of resistance to Fusarium head blight of wheat. Plant Breeding 114:377–386.Google Scholar
  29. Mesterházy Á (2002)Role of deoxynivalenol in aggressiveness of Fusarium graminearum and F. culmorum and in resistance to Fusarium head blight. European Journal of Plant Pathology 108:675–684.Google Scholar
  30. Mesterházy Á, Bartó k T, Mirocha CG and Komoróczy R (1999) Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breeding 118:97–110.Google Scholar
  31. Miller JD, Simon JW, Blackwell BA, Greenhalgh R and Taylor A. (1999) Deoxynivalenol: A 25 year perspective on a trichothecene of agricultural importance. In: Summerell BA, Leslie JF, Backhouse D, Bryden WL and Burgess LW (eds)Fusarium (pp 310–320)APS Press, St.Paul, MN, USA.Google Scholar
  32. Miller JD, Young JC and Sampson DR (1985)Deoxynivalenol and Fusarium head blight resistance in spring cereals. Phytopathologie Zeitschrift 113:359–367.Google Scholar
  33. Mohammadi M, Kazemi H (2002)Changes in peroxidase and polyphenol oxidase activities in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance. Plant Science 162:491–498.Google Scholar
  34. Proctor RH, Hohn TM and McCormick SP (1995)Reduced virulence of Gibberella zeae caused by disruption of a trichothecene toxin biosynthetic gene. Molecular Plant – Microbe Interactions 8:593–601.Google Scholar
  35. Ribichich KF, Lopez SE and Vegetti AC (2000)Histopathological spikelet changes produced by Fusarium graminearum in susceptible and resistant wheat cultivars. Plant Disease 84:794–802.Google Scholar
  36. SAS Institute Inc.(1988)SAS/STAT user's guide: release 6.03. SAS Institute Inc., Cary,1029 pp.Google Scholar
  37. Savard ME, Sinha RC, Seaman WL and Fedak G (2000) Sequential distribution of the mycotoxin deoxynivalenol in wheat spikes after inoculation with Fusarium graminearum. Canadian Journal of Plant Pathology 22:280–285.Google Scholar
  38. Schroeder RW and Christensen JJ (1963)Factors a effecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838.Google Scholar
  39. Singh RP and van Ginkel M (1997)Breeding strategies for introgressing diverse scab resistance into adapted wheats. In: Duben HJ, Gilchrist L, Reeves J and McNab A (eds) Fusarium Head Scab: Global Status and Future Prospects (pp 86–92) CIMMYT, Mexico.Google Scholar
  40. Siranidou E, Kang Z and Buchenauer H (2002)Studies on symptom development, phenolic compounds and morphological defense responses in wheat cultivars differing in resistance to Fusarium head blight. Journal of Phytopathology 150:200–208.Google Scholar
  41. Snijders CHA and Perkowski J (1990)Effects of head blight caused by Fusarium culmorum on toxin production and weight of wheat kernels. Phytopathology 80:566–570.Google Scholar
  42. Snijders CHA and Krechting CF (1992)Inhibition of deoxynivalenol translocation and fungal colonization in Fusarium head blight resistant wheat. Canadian Journal of Botany 70: 1570–1576.Google Scholar
  43. Ueno Y (1977)Trichothecenes: Overview address. In: Rodricks JV, Hesseltine CW and Mehlman MA (eds)Mycotoxins in Human and Animal Health.(pp 189–207)Pathotox Publishers Inc., Park Forest South, USA.Google Scholar
  44. Wang YZ and Miller JD (1988)Effects of Fusarium graminearum metabolites on wheat tissue in relation to Fusarium head blight resistance. Journal of Phytopathology 122:118–125.Google Scholar
  45. Windels CE (2000)Economic and social impacts of Fusarium head blight: Changing farms and rural communities in the Northern Great Plains. Phytopathology 90:17–21.Google Scholar
  46. Wong LSL, Abramson D, Tekauz A, Leisle D and McKenzie RIH (1994)Pathogenicity and mycotoxin production of Fusarium species causing head blight in wheat cultivars varying in resistance. Canadian Journal of Plant Science 75: 261–267.Google Scholar
  47. Zhu H, Gilchrist L, Hayes P, Kleinhofs A, Kudrna D, Liu Z, Prom L, Steffenson B, Toojinda T and Vivar H (1999)Does function follow form? Principal QTLs for Fusarium head blight (FHB)resistance are coincident with QTLs for in fluorescence traits and plant height in a doubled-haploid population of barley. Theoretical and Applied Genetics 99: 1221–1232.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • François Langevin
    • 1
  • François Eudes
    • 1
  • André Comeau
    • 1
  1. 1.Agriculture and Agri-Food Canada, Sainte-Foy Research Centre, 2560 Hochelaga Boul, Sainte-Foy, Québec G1V 2J3Canada

Personalised recommendations