European Journal of Plant Pathology

, Volume 110, Issue 5–6, pp 551–562

Genetic Diversity of Fusarium Graminearum in Europe and Asia

  • T.Yu. Gagkaeva
  • T. Yli-Mattila
Article

Abstract

The biodiversity and phylogeny of Fusarium graminearum isolates originating from different geographical areas was analyzed using isozyme variation, β-tubulin and IGS sequences. Geographically distinct groups of isolates originating from different areas of the Russian Federation, China, Germany and Finland were studied. The complex enzymes (α- and β-esterases, aspartate aminotransferase and superoxide dismutase) and the IGS sequences demonstrated a high level of genetic diversity in F. graminearum. Diversity in the Asian population was higher than in the European one. There was a correlation between genetic clusters of the IGS sequences and geographical origin in F. graminearum. Knowledge of biodiversity and identification of some phylogenetic lineages in F. graminearum will be useful in defining the risk of pathogen evolution as well as benefiting disease management strategies.

IGS isozyme population β-tubulin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aoki T and O'Donnell K (1999) Morphological and molecular characterization of Fusarium pseudograminearum sp. nov., formerly recognized as the group 1 population of F. graminearum. Mycologia 91: 597-609Google Scholar
  2. Bai GH and Shaner G (1996) Variation in Fusarium graminearum and cultivar resistance to wheat scab. Plant Disease 80: 975-979Google Scholar
  3. Bakan B, Cahagnier B and Melcion D (2001) Natural occurrence of Fusarium toxins in domestic wheat and corn harvested in 1996 and 1997-production of mycotoxin by Fusarium isolates from these samples. In: Logrieco A (ed.) Cost Action 835 Occurrence of Toxigenic Fungi and Mycotoxins in Plants, Food and Feed in Europe (pp 51–53) European Communities, BelgiumGoogle Scholar
  4. Bottalico A and Perrone G (2002) Toxigenic Fusarium species and mycotoxins associated with head blight in small grain cereals in Europe. European Journal of Plant Pathology 198: 611-624Google Scholar
  5. Bowden RL, Zeller KA and Leslie JF (2000) Population structure of Gibberella zeae in the Great Plains of North America. In: Proceedings of the International Symposium on Wheat Improvement for Scab Resistance (pp 211-213) 5–11 May 2000, ChinaGoogle Scholar
  6. Chen LF, Bai GH and Desjardins AE (2000) Recent advances in wheat head scab researches in China. In: Proceedings of the International Symposium on Wheat Improvement for scab Resistance (pp 258-273) 5–11 May 2000, ChinaGoogle Scholar
  7. Cosic J and Jurkovic D (2000) Biological characterization of Fusarium graminearum Schw. Isolated from different host plants. In: 6th European Fusarium Seminar and Third COST 835 Workshop of Agriculturally Important Toxigenic Fungi, Book of Abstracts: 79–80Google Scholar
  8. Cullen D, Caldwell RW and Smalley EB (1982) Cultural characteristics, pathogenicity and zearalenone production by strains of Gibberella zeae isolated from corn. Phytopathology 72: 1415-1418Google Scholar
  9. Dusabenyagasani M, Dostaler D and Hamelin RC (1999) Genetic diversity among Fusarium graminearum strains from Ontario and Quebec. Canadian Journal of Plant Pathology 21: 308-314Google Scholar
  10. Ellner FM (2001) Levels of mycotoxins in cereals of various regions of Germany in the 1990 harvest. In: Logrieco A (ed.) Cost Action 835 Occurrence of Toxigenic Fungi and Myco-toxins in Plants, Food and Feed in Europe (pp 59–60). European Communities, BelgiumGoogle Scholar
  11. Fernando WGD, Ramarathnam J, Gilbert J and Clear R (2003) Genetic diversity of Fusarium graminearum isolates from wheat, barley, and corn. In: Proceedings of International Congress of Plant Pathology (pp 107-?) 2–8 February 2003, New ZealandGoogle Scholar
  12. Felsenstein (1993). ?Google Scholar
  13. Gagkaeva TY, Koopmann B and Wolf GA (2001) Biodiversity of F. graminearum isolates from different geographical locations. In: Proceedings of the 8th Aschersleben Symposium ''New Aspects of Resistance Research on Cultivated Plants'' (pp 11-15) GermanyGoogle Scholar
  14. Gagkaeva TY, Levitin MM, Zuev E and Terentjeva I (2002) Evaluation of genetic resources of wheat and barley from far east of Russia for resistance to Fusarium head blight. Journal Applied Genetics 43A: 229-236Google Scholar
  15. Gale LR, Chen L-F, Hernick CA, Takamura K. Kistler HC (2002) Population analysis of Fusarium graminearum from wheat fields in eastern China. Phytopathology 92: 1315-1322Google Scholar
  16. Gladstein and Wheeler (2001). ?Google Scholar
  17. Gocho H (1985) Wheat breeding for scab resistance. Wheat Information Service 60: 41--Google Scholar
  18. Honold GR, Farkas GL and Stahmann MA (1966) The oxidation-reduction enzymes of wheat. Cereal Chemistry 43: 517-529Google Scholar
  19. Ittu M (2001) Occurrence of FHB in Romania and control strategy. In: Logrieco A (ed.) Cost Action 835 Occurrence of Toxigenic Fungi and Mycotoxins in Plants, Food and Feed in Europe (pp 147–150) European Communities, BelgiumGoogle Scholar
  20. Klechkovskaya H (1999) Ecological and biochemical charac-teristics of Fusarium spp. on winter wheat in steppe area close to Black Sea in Ukraine. Mycology and Phytopathology 33: 280–289 (in Russian)Google Scholar
  21. Koixumi S, Kato H, Yoshino R, Hayoshi N and Ichinoe M (1991) Distribution of causal fusaria of wheat and barley scab in Japan. Annals of the Phytopathological Society of Japan 57: 165-173Google Scholar
  22. Korochkin LI, Serov OL and Pudovkin AI (1977) Genetic of enzymes. Moscow, Russia: 1–278 (in Russian)Google Scholar
  23. Laday M, Bagi F, Mesterhazy A and Szecsi A (2000) Isozyme evidence for two groups of Fusarium graminearum. Mycological Research 104: 788-793Google Scholar
  24. Lee SB, Taylor JW (1990) Isolation of DNA from fungal mycelia and single spores. In: Innis MM, Gelfand DH, Sninsky JJ and White JW (eds) PCR Protocols, a Guide to Methods and Applications (pp 282–287) Academic Press, San Diego, USAGoogle Scholar
  25. Levitin MM, Ivashchenko VG, Shipilova NP, Gagkaeva TY (1994) Fusarium head blight of cereals and problems of breeding for resistance. Plant Science (Sofia) 31: 158-161Google Scholar
  26. Levitin M, Ivashenko V, Shipilova N and Gagkaeva T (2000) Fusarium head blight of the cereal crops in Russia. Plant Protection 51: 111-122Google Scholar
  27. Lew H, Adler A, Thimm N, Kriska G and Schuh M (2001) Occurrence of toxigenic fungi and related mycotoxins in plants, food and feed in Austria. In: Logrieco A (ed.) Cost Action 835 Occurrence of Toxigenic Fungi and Mycotoxins in Plants, Food and Feed in Europe (pp 3–12) European Communities, BelgiumGoogle Scholar
  28. Liu W-Ch, Xi J-H, Li H-Y, Pan H-Yu, Hu H-Q, Guo Y-L and Bai R-L (2002) RAPD analysis of isolates from Fusarium spp. causing wheat head blight in northeast China. Mycosystema 21: 63-70Google Scholar
  29. Logrieco A, Altomare C, Mule G and Moretti A (1999) Molecular and biochemical characterization of toxigenic Fusarium species. Recent Research Developments in Microbiology 3: 135-145Google Scholar
  30. Mayrer G (1971) Disk-Electrophoresis. Moscow, Russia: 1–247 (in Russian)Google Scholar
  31. Miedaner T, Schilling AG and Geiger HH (2001) Molecular genetic diversity and variation for aggressiveness in populations of Fusarium graminearum and F. culmorum sampled from wheat fields in different countries. Journal of Phytopathology 149: 641-648Google Scholar
  32. Mladenov M and Karadzhova I (1978) Investigation of Fusarium species on wheat in Bulgaria. Rastenievdni Nauk 15: 170-175Google Scholar
  33. Möller EM, Bahnweg G, Sandermann H and Geiger HH (1992) A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies and infected plant tissue. Nucleic Acids Research 20: 6115-6116PubMedGoogle Scholar
  34. Nicholson P, Carter JP, Chandler E and Simpson S (2002) Pathogenicity and genetic diversity in Fusarium graminearum and relationship to nivalenol and deoxynivalenol. In: Abstracts of 7th European seminar on Fusarium-mycotoxins, taxonomy and pathogenicity and WG-4 COST 835 Action Workshop (pp 18-?) Poznan, PolandGoogle Scholar
  35. O'Donnell K, Cigelnik E and Casper HH (1998) Molecular, phylogenetic, morphological, and mycotoxin data support reidentification of the Quorn mycoprotein fungus as Fusarium venenatum. Fungal Genetics and Biology 23: 57-67CrossRefPubMedGoogle Scholar
  36. O'Donnell K, Kistler, HC, Tacke BK and Casper HH (2000) Gene genealogies reveal global phylogeographic structure and reproductive isolation among lineages of Fusarium graminearum, the fungus causing wheat scab. Proceedings of the National Academy of Sciences, USA 97: 7905-7910CrossRefGoogle Scholar
  37. Ostry V and Ruprich J (2001) Fumonisins in corn-based products and Fusarium occurrence in wheat grains in the Czech Republic. In: Logrieco A (ed.) Cost Action 835 Occurrence of Toxigenic Fungi and Mycotoxins in Plants, Food and Feed in Europe (pp 25–36) European Communities, BelgiumGoogle Scholar
  38. Oswald J W (1949) Cultural variation, taxonomy and pathogenicity of Fusarium species associated with cereal root rots. Phytopathology 39: 359-376Google Scholar
  39. Paavanen-Huhtala et al. (1999). ? Paavanen-Huhtala S, Avikainen H and Yli-Mattila T (2000) Development of strain-specific primers for a strain of Gliocladium catenulatum used in biological control. European Journal of Plant Pathology 106: 187–198Google Scholar
  40. Paljchevskiy NA (1891) Disease of cereal grain in the South-Ussuriiskiy region. SPb, Russia: 1–43 (in Russian)Google Scholar
  41. Proctor RH, Hohn TM and McCormick SP (1995) Reduced virulence of Gibberella zeae caused by disruption of a trichothecene toxin biosynthetic gene. Molecular Plant-Microbe Interactions 8: 593-601PubMedGoogle Scholar
  42. Quellet T and Seifert KA (1993) Genetic characterization of Fusarium graminearum strains using RAPD and PCR amplification. Phytopathology 83: 1003-1007Google Scholar
  43. Reddy MN and Stahmann MA (1972) Isozyme patterns of Fusarium species and their significance in taxonomy. Phytopathologische Zeitschrift 74: 115-125Google Scholar
  44. Roux J, Steenkamp ET, Marasas WFO, Wingfield MJ and Wingfield BD (2001) Characterization of Fusarium graminearum from Acacia and Eucalyptus using beta-tubulin and histone gene sequences. Mycologia 93: 704-711Google Scholar
  45. Schilling AG, Moller EM and Geiger HH (1997) Molecular differentiation and diagnosis of the cereal pathogens Fusarium culmorum and F. graminearum. Sydowia 30: 71-82Google Scholar
  46. Snijders CA (1990) Genetic variation for resistance to Fusarium head blight in bread wheat. Euphytica 50: 171-179Google Scholar
  47. Sugiura Y, Watanabe Y, Tanaka T, Yamamoto S and Ueno Y (1990) Occurrence of Gibberella zeae strains that produce both nivalenol and deoxynivalenol. Applied and Environ-mental Microbiology 56: 3047-3051Google Scholar
  48. Szersi A, Szentkiralyi F and Koves-Pechy Ch (1976) Compar-ison of esterase patterns of Fusarium culmorum and Fusarium graminearum. Acta Phytopathologica 11: 183-203Google Scholar
  49. Takeda K and Heta H (1989) Establishing the testing method and a search for resistant varieties to Fusarium head blight in barley. Japanese Journal Breeding 39: 203-216Google Scholar
  50. Thompson JD, Higgins DG and Gibson TJ (1994) Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673-4680PubMedGoogle Scholar
  51. Voronin M (1890) About intoxicating corn in the South-Ussuriiskij region. Botanical notes. SPb, Russia 3: 13–21(in Russian).Google Scholar
  52. Voronin M (1891) Ueber das “Taumelgetreide” in Sud-Ussurien. Botanische Zeitung, Leipzig 49: 81–93.Google Scholar
  53. Walker SL, Leath S, Hagler WMJ and Murphy JP (2001) Variation among isolates of Fusarium graminearum associated with Fusarium head blight in North Carolina. Plant Disease 85: 404–410.Google Scholar
  54. Wheeler WC (1996) Optimization alignment. The end of multiple sequence alignment in phylogenetics? Cladistics 12: 1–9.CrossRefGoogle Scholar
  55. Ylimäki, A, Koponen, H, Hintikka, E-L, Nummi, M, Niku-Paavola, M-L, Ilus, T, Enari, TM (1979) Mycoflora and occurrence of Fusarium toxins in Finnish grain. In: Technical Research Centre of Finland, Materials and Processing Technology Publication, Vol. 21 pp. 1–28, Valtion Painatuskeskus, Helsinki, Finland.Google Scholar
  56. Yli-Mattila T, Paavanen-Huhtala S, Hannukkala A, Papikka P, Tahvonen R and Karjalainen R (1996) Isozyme and RAPDPCR analyses of Fusarium avenaceum strains from Finland. Plant Pathology 45: 126–134.CrossRefGoogle Scholar
  57. Yli-Mattila T, Paavanen-Huhtala S, Bulat SA, Alekhina IA and Nirenberg HI (2002a) Molecular, morphological and phylogenetic analysis of Fusarium avenaceum/F. arthrosporioides/F. tricinctum species complex — a polyphasic approach. Mycological Research 106: 655–669.CrossRefGoogle Scholar
  58. Yli-Mattila T, Paavanen-Huhtala S, Parikka P, Konstantinova P, Gagkaeva T, Eskola M, Jestoi M and Rizzo A (2002b) Occurrence of Fusarium fungi and their toxins in Finnish cereals in 1998 and 2000. Journal of Applied Genetics 43A: 207–214.Google Scholar
  59. Yli-Mattila T, Mach R, Alekhina IA, Bulat SA, Koskinen S, Kullnig-Gradinger CM, Kubicek C. and Klemsdal SS (in press) Phylogenetic relationsip of Fusarium langsethiae to Fusarium poae and F. sporotrichioides as inferred by IGS, ITS, β-tubulin sequence and UP-PCR hybridization analysis. International Journal of Food Microbiology.Google Scholar
  60. Zheng YM, Lin ZF and Zhu ZD (1983) Study on pathogenic species and form of wheat scab fungi in Fijian Province. Acta Phytopathologica Sinica 13: 53–59.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • T.Yu. Gagkaeva
    • 1
  • T. Yli-Mattila
    • 2
  1. 1.Laboratory of Mycology and PhytopathologyAll-Russian Institute of Plant Protection (VIZR)St-Petersburg-PushkinRussia; Fax:
  2. 2.Department of Biology, Laboratory of Plant Physiology and Molecular BiologyUniversity of TurkuFinland

Personalised recommendations