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Variability of Cuban and International Populations of Alternaria solani from Different Hosts and Localities: AFLP Genetic Analysis

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Abstract

As causal agent of early blight disease in tomato and potato, Alternaria solani is an internationally important horticultural pathogen. Genetic variability was surveyed by amplified fragment length polymorphism analysis in a total of 112 isolates from potato and tomato, representing pathogen populations from different Cuban provinces together with isolates from the USA, Brazil, Turkey, Greece and China. Also included in the analysis were isolates from catenulated Alternaria spp. from Brazil, Canada, Greece and Russia, along with single isolates of Alternaria porri, Alternaria alternata and a Curvularia sp. UPGMA clustering revealed a differentiation between the isolates of A. solani and all other species with the exception of A. porri which could not be distinguished from A. solani. Among the isolates of A. solani, two distinct subclusters were formed, with high genetic significance revealed by bootstrapping, corresponding to a general subdivision based on the respective solanaceous host. The results are discussed with regard to potential host specificity of A. solani on tomato and potato, and in terms of the comparative contributions of regional and international genetic variability in populations of this ubiquitous plant pathogen.

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References

  • Adachi Y, Watanabe H and Tsuge T (1996) Relationships between genetic polymorphisms and fungicide resistance within Alternaria alternata. Phytopathology 86: 1248–1254.

    Google Scholar 

  • Akamatsu H, Taga M, Kodama M, Johnson M, Otani R and Kohmoto K (1999) Molecular karyotypes for Alternaria plant pathogens known to produce host-specific toxins. Current Genetics 35: 647–656.

    Article  PubMed  Google Scholar 

  • Andersen B, Kroger E and Roberts RG (2001) Chemical and morphological segregation of Alternaria alternata, A. gaisen and A. longipes. Mycological Research 105: 291–299.

    Article  Google Scholar 

  • Aradhya MK, Chan HM and Parfitt DE (2001) Genetic variability in the pistachio late blight fungus, Alternaria alternata. Mycological Research 105: 300–306.

    Article  Google Scholar 

  • Bock CH, Thrall PH, Brubaker CL and Burdon JJ (2002) Detection of genetic variation in Alternaria brassicicola using AFLP fingerprinting. Mycological Research 106: 428–434.

    Article  Google Scholar 

  • Bonde R (1929) Physiological strains of Alternaria solani. Phytopathology 19: 533–548.

    Google Scholar 

  • Bruner SC (1918) La pudrición del tomate y modo de evitarla. Revista de Agricultura, Comercio y Trabajo 1: 300–303.

    Google Scholar 

  • Ellis JB and Martin GB (1882) New species of North American fungi: Macrosporium solani. American Naturalist 16: 1001–1004.

    Google Scholar 

  • Ellis MB (1971) Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, FAO, Kew, Surrey.

    Google Scholar 

  • Esquivel EA (1984) Pleospora solani sp-nov, telemorphosis of Alternaria solani (Ell and Mart) Jones and Grout. Phytopathology 74: 1014.

    Google Scholar 

  • Excoffier L, Smouse PE and Quattro JM (1992) Analysis of molecular variance inferred from metric distance among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics 131: 479–491.

    PubMed  Google Scholar 

  • Franco J, Crossa J, Ribaut JM, Betran J, Warburton ML and Khairallah M (2001) A method for combining molecular markers and phenotypic attributes for classifying plant genotypes. Theoretical and Applied Genetics 103: 944–952.

    Article  Google Scholar 

  • Gordon TR and Martyn RD (1997) The evolutionary biology of Fusarium oxysporum. Annual Reviews of Phytopathology 35: 111–128.

    Article  Google Scholar 

  • Izquierdo F (1981) Evidencia de la existencia de razas fisiológicas en Alternaria solani. Revista de Ciencias Biológicas 12: 223–233.

    Google Scholar 

  • Martínez B, Bernal A, Pérez S and Munis Y (2002) Variabilidad patogénica de aislamientos en Alternaria solani Sor. Revista de Proteccion Vegetal 17: 45–53.

    Google Scholar 

  • McDonald BA (1997) The population genetics of fungi: Tools and techniques. Phytopathology 87: 448–453.

    Google Scholar 

  • Morris PF, Connolly MS and St Clair DA (2000) Genetic diversity of Alternaria alternata isolated from tomato in California assessed using RAPDs. Mycological Research 104: 286–292.

    Article  Google Scholar 

  • Mukherji S (1961) Untersuchungen zur Änderung der Pathogenität von Alternaria porri (Ell.) Saw. f. sp. solani E. et M. Journal of Phytopathology 41: 317–352.

    Google Scholar 

  • Neergaard P (1945) Danish Species of Alternaria and Stemphylium. Taxonomy, Parasitism, Economical Significance. Humphrey Millford, Oxford University Press, London.

    Google Scholar 

  • Peever TL, Olsen L, Ibañes A and Timmer LW (1999) Genetic differentiation and host specificity among populations of Alternaria spp. causing brown spot of Grapefruit and Tangarina × Grapefruit hybrids in Florida. Phytopathology 90: 407–414.

    Google Scholar 

  • Pei MH and Ruiz C (2000) AFLP evidence of the distinctive patterns of life-cycle in two forms of Melampsora rust on Salix viminalis. Mycological Research 104: 937–942.

    Article  Google Scholar 

  • Petrunak DM and Christ BJ (1992) Isozyme variability in Alternaria solani and Alternaria alternata. Phytopathology 82: 1343–1347.

    Google Scholar 

  • Pryor BM and Gilbertson RL (2000) Molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear ITS and mt SSU rDNA sequences. Mycological Research 104: 1312–1321.

    Article  Google Scholar 

  • Roberts RG, Reymond ST and Andersen B (2000) RAPD fragment pattern analysis and morphological segregation of smallspored Alternaria species and species groups. Mycological Research 104: 151–160.

    Article  Google Scholar 

  • Rotem J (1966) Variability in Alternaria porri f.sp. solani. Israel Journal of Botany 15: 48–57.

    Google Scholar 

  • Rotem J (1994) The Genus Alternaria: Biology, Epidemiology and Pathogenicity. APS Press, St. Paul, Minnesota.

    Google Scholar 

  • Salamiah A, Akamatsu H, Fukumasa-Nakai H, Otani H and Kodama M (2001) Construction and genetic analysis of hybrid strains between apple and tomato pathotypes of Alternaria alternata by protoplast fusion. Journal of General Plant Pathology 67: 97–105.

    Google Scholar 

  • Sambrook J, Fritsh EF and Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Laboratory Press, New York.

    Google Scholar 

  • Sharma TRT and Tewari JP (1998) RAPD analysis of three Alternaria species pathogenic to crucifers. Mycological Research 102: 807–814.

    Article  Google Scholar 

  • Simmons EG (1992) Alternaria taxonomy: Currents status, viewpoint, challenge. In: Chelkowski J and Visconti A (eds) Alternaria: Biology, Plant Diseases and Metabolites. Elsevier Science Publishers, Amsterdam, pp. 1–35.

    Google Scholar 

  • Simmons EG (2000) Alternaria themes and variations (244-286), species on solanaceae. Mycotaxon, LXXV: 1–115.

    Google Scholar 

  • Stancheva Y (1989) Investigations of the phytotoxic activity of culture filtrates of different isolates of Alternaria solani. Rasteniev'dni-Nauki 26: 97–101.

    Google Scholar 

  • Tsuge T, Hayashi N and Nishimura S (1987) Selection of auxotrophic mutants and heterokaryosis in Alternaria alternata. Annals of the Phytopathological Society of Japan 53: 182–190.

    Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J and Kuiper M (1995) AFLP: A new technique for DNA fingerprinting. Nucleic Acids and Research 23: 4407–4414.

    Google Scholar 

  • Weir TL, Huff DR, Christ BJ and Romaine CP (1998) RAPD-PCR analysis of genetic variation among isolates of Alternaria solani and Alternaria alternata from potato and tomato. Mycologia 90: 813–821.

    Google Scholar 

  • Yap IV and Nelson RJ (1996) WinBoot: A program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrograms. IRRI Discussion Paper Series No. 14, International Rice Research Institute, Manila.

    Google Scholar 

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Authors and Affiliations

  1. Dpto, Fitopatología-Centro Nacional de Sanidad Agropecuaria (CENSA), Carretera de Tapaste y Autopista Nacional, San J. de las Lajas, Habana, Cuba

    Simón Pérez Martínez

  2. Institut für Pflanzenbau und Pflanzenzüchtung, Justus-Liebig-Universität, Heinrich-Buff-Ring 26-32, D-35392, Giessen, Germany

    Rod Snowdon & Jörn Pons-Kühnemann

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  1. Simón Pérez Martínez
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  2. Rod Snowdon
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  3. Jörn Pons-Kühnemann
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Correspondence to Jörn Pons-Kühnemann.

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Pérez Martínez, S., Snowdon, R. & Pons-Kühnemann, J. Variability of Cuban and International Populations of Alternaria solani from Different Hosts and Localities: AFLP Genetic Analysis. European Journal of Plant Pathology 110, 399–409 (2004). https://doi.org/10.1023/B:EJPP.0000021071.65146.c0

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  • DOI: https://doi.org/10.1023/B:EJPP.0000021071.65146.c0

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