European Journal of Plant Pathology

, Volume 110, Issue 2, pp 139–154

Pathogenic and Genetic Diversity of Soilborne Isolates of Cylindrocladium from Banana Cropping Systems

  • Jean-Michel Risède
  • Philippe Simoneau
Article

Abstract

Pathogenicity and genetic variation were investigated within a collection of 104 banana-infecting isolates of Cylindrocladium (teleomorph Calonectria) originating from different countries and representing previously described morphological taxa or species. These root-rot fungi, along with endoparasitic nematodes, have been reported to be causal agents of necrotic lesions that induce root breakage and toppling of banana plants. Little is known about the individual pathogenic effects of the species involved or their genetic diversity. In the present study, among the five morphological taxa found in the banana rhizosphere, only isolates showing an atypical morphology relative to Cylindrocladium gracile (named Cy. gracile-like isolates) and Cy. spathiphylli isolates were pathogenic on banana cultivar Grande Naine. When comparing the latter isolates with others of the same species, but originating from different hosts, an analysis of rDNA spacer polymorphism partitioned isolates of Cy. spathiphylli by host into a banana – tea group and a HeliconiaSpathiphyllum group. Furthermore, isolates from Heliconia were not pathogenic on banana. A pathogenicity assessment of representative isolates from the Cy. gracile-like and the Cy. spathiphylli taxa on six different banana cultivars yielded no evidence of differential interactions between isolates and banana genotypes. Significant differences in susceptibility between banana genotypes were nevertheless detected that could potentially be exploited by breeders. Random amplified polymorphic DNA analysis revealed a genetic similarity ranging from 70% to 100% within Cy. spathiphylli isolates from bananas regardless of the geographic origin. Moreover, Cy. gracile-like isolates were highly similar but showed only 60% similarity relative to the Cy. gracile reference isolates, thus raising questions about their species status.

Calonectria pathogenic variation disease severity Heliconia RAPD rDNA spacers 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Castaing V, Beveraggi A, Fouré E and Fogain R (1996) Detection of a Cylindrocladium sp. in Cameroon. Studies on pathogenic activity and interactions with R. similis. Infomusa 5: 4-7Google Scholar
  2. Crous PW (2002) Taxonomy and Pathology of Cylindrocladium (Calonectria) and Allied Genera, APS Press, 294 p, St. Paul, Minnesota, USAGoogle Scholar
  3. Crous PW and Peerally A (1996) Gliocladiopsis irregularis sp. nov. and notes on Cylindrocladium spathiphylli. Mycotaxon 65: 119-128Google Scholar
  4. Crous PW and Wingfield MJ (1994) A monograph of Cylindrocladium including anamorphs of Calonectria. Mycotaxon 51: 341-435Google Scholar
  5. Declerck S, Risede JM, Rufyikiri G and Delvaux B (2002) Effects of arbuscular mycorrhizal fungi on severity of root rot of bananas caused by Cy. spathiphylli. Plant Pathology 51: 109-115Google Scholar
  6. Duchesne LC and Anderson JB (1990) Location and direction of transcription of the 5S rRNA in Armillaria. Mycological Research 94: 266-269Google Scholar
  7. Felsenstein J (1995) PHYLIP (Phylogeny Inference Package) version 3.57c. Seattle: University of Washington, Department of Genetics, USA. Distributed by the authorGoogle Scholar
  8. Goodwin DC and Lee SB (1993) Microwave miniprep of total genomicDNAfrom fungi, plants, protists and animals for PCR. Biotechniques 15: 438-444Google Scholar
  9. Gowen S and Queneherve P (1990) Nematode parasites of bananas, plantains and abaca. In: Luc et al. (eds) Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (pp 431-460) CAB International, Wallingford, Oxon, UKGoogle Scholar
  10. Jeng RS, Dumas M, Liu FH, Wang CL and Hubbes M(1997)DNA analysis of Cylindrocladium floridanum isolates from selected forest nurseries. Mycological Research 101: 285-291Google Scholar
  11. Jenny C, Carreel F, Tomekpe K, Perrier X, Dubois C, Horry JP and Tezenas du Montcel H (1999) Les bananiers. In: Hamon et al. (eds) Diversité génétique des Plantes Cultivées (pp 113-139) Montpellier, FranceGoogle Scholar
  12. Jones DR (1999) Diseases of Banana, Abaca and Enset, CAB International, Wallingford, Oxon, UKGoogle Scholar
  13. Kang JC, Crous PW and Schoch CL (2001) Species concepts in the Cylindrocladium floridanum and Cy. spathiphylli complexes (Hypocreaceae) based on multi-allelic sequence data, sexual compatibility and morphology. Systematic and Applied Microbiology 24: 206-217Google Scholar
  14. Kobenan K (1991) Parasites et ravageurs des bananiers en Côte d'Ivoire. Fruits 46: 633-641Google Scholar
  15. Lassoudière A (1978) Quelques aspects de la croissance et du développement du bananier Poyo en Côte d'Ivoire. Le système radical. Fruits 33: 314-338Google Scholar
  16. Loridat P (1989) Etude de la microflore fongique et des nématodes associés aux nécroses de l'appareil souterrain du bananier en Martinique. Mise en évidence du pouvoir pathogène du genre Cylindrocladium. Fruits 44: 587-597Google Scholar
  17. Peerally A (1991) The classification and phytopathology of Cylindrocladium species. Mycotaxon 40: 323-366Google Scholar
  18. Perrière G and Gouy M(1996)WWW-Query: An on-line retrieval system for biological sequence banks. Biochimie 78: 364-369Google Scholar
  19. Price NS (1995) Banana morphology-Part I: Roots and rhizomes. In: Gowen Chapman et al. (eds) Bananas and Plantains (pp 179-189) London, UKGoogle Scholar
  20. Risède JM (1994) Eléments de caractérisation de Cylindrocladium sp. agent de nécroses racinaires du bananier en Martinique. Fruits 49: 167-178Google Scholar
  21. Risède JM (1995) Spatial and temporal distribution in soils of Cylindrocladium sp. fungal pathogen of bananas. Phytopathology 85: 1563Google Scholar
  22. Risède JM and Simoneau P (2001) Typing Cylindrocladium species by analysis of ribosomal DNA spacers polymorphism: Application to field isolates from the banana rhizosphere. Mycologia 93: 494-504Google Scholar
  23. Rossman AY (1979) Calonectria and its type species, C. daldiniana, a later synonym of C. pyrochroa. Mycotaxon 8: 321-328Google Scholar
  24. Rossman AY (1983) The phragmosporous species of Nectria and related genera. Mycological Papers 150: 1-164Google Scholar
  25. Schoch CL and Crous PW (1999) First report of Cylindrocladium root and petiole rot to Spathiphyllum in South Africa. South African Journal of Botany 65: 208-211Google Scholar
  26. Schoch CL, Crous PW, Wingfield BD and Wingfield MJ (2001) Phylogeny of Calonectria based on comparisons of β-tubulin DNA sequences. Mycological Research 105: 1045-1052Google Scholar
  27. Schoulties CL, El-Gholl NE and Alfieri SA (1982) Cy. spathiphylli sp. nov. Mycotaxon 16: 265-272Google Scholar
  28. Semer CR, Mitchell DJ, Mitchell ME, Martin FR and Alfenas AC (1987) Isolation, identification and chemical control of Cylindrocladium musae sp. nov. associated with toppling disease of banana. Phytopathology 77: 1729Google Scholar
  29. Sokal RRR and Michener CD (1958) A statistical method for evaluation of systematic relationships. University of Kansas Science Bulletin 38: 1409-1438Google Scholar
  30. Stover RH and Simmonds NW (1987) Bananas, 3rd edn. 468 p, Longman Scientific & Technical, Harlow, UKGoogle Scholar
  31. Thies WG and Patton RF (1970) Biology of Cylindrocladium scoparium in Wisconsin forest tree nurseries. Phytopathology 60: 1662-1668Google Scholar
  32. Thompson JD, Higgins DG and Gibson TJ (1994) CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673-4680Google Scholar
  33. Uchida JY (1989) Cylindrocladium rot of Spathiphyllum. Hawaii Institute of Tropical Agriculture and Human Resources Brief 78: 1-4Google Scholar
  34. Uchida JY and Aragaki M (1992) Further characterization of Cy. spathiphylli from Spathiphyllum in Hawaii and Florida. Mycologia 84: 810-814Google Scholar
  35. Uchida JY, Aragaki M and Yahata PS (1989). Heliconia root rot and foliar blight caused by Cylindrocladium. Hawaii Institute of Tropical Agriculture and Human Resources Brief 85: 1-2Google Scholar
  36. White TJ, Bruns T, Lee S and Taylor J (1990) Amplification and direct sequencing of fungal ribosomal genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ and White TJ (eds) PCR Protocols: A Guide to Methods and Applications (pp 315-322) Academic Press, San DiegoGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Jean-Michel Risède
    • 1
  • Philippe Simoneau
    • 2
  1. 1.Laboratoire de Pathologie végétale, Station de Neufchâteau, CIRAD-FLHORCapesterre Belle-eau, Guadeloupe (F.W.I) (Fax
  2. 2.UMR de Pathologie Végétale, Faculté des SciencesUniversité d'AngersAngersFrance

Personalised recommendations