Digestive Diseases and Sciences

, Volume 49, Issue 6, pp 925–930 | Cite as

Gastric and Duodenal Mucosal Protein Fractional Synthesis and Growth Factor Expression in Patients with H. pylori-Associated Gastritis Before and After Eradication of the Organism

  • Trevor A. Winter
  • Mark Kidd
  • Philip Kaye
  • I. N. (Solly) Marks
Article

Abstract

Our purpose was to study the effect of Helicobacter pylori (HP) on mucosal protein fractionalsynthesis (MPFS) and growth factor expression. 14 C-leucine incorporation, and TGF-α, β-FGF, andEGF-receptor levels were assessed in gastric and duodenal mucosa in 20 patients with HP-associatedgastritis and repeated after treatment of the gastritis, with or without eradication of the organism.At entry, MPFS in the fundus, antrum, and duodenum was 43.1, 38.2, and 28.3%/day, respectively.Following HP eradication, fundal and antral rates fell to 28.1 and 21.4%/day ( P < 0:05), whereasthe duodenum was unchanged. MPFS in the patient subset not eradicated remained similar to entryvalues (35.9, 31.6, and 25.4%/day). Expression of TGF-α, β-FGF, and EGFreceptors was unchanged.Eradication of HP results in reduction of gastric, but not duodenal, MPFS and has no effect on thegrowth factors measured. Increased MPFS associated with HP gastritis may relate to the potentialfor neoplastic transformation.

Helicobacter pylori gastritis mucosal protein fractional synthesis growth factors 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

REFERENCES

  1. 1.
    Parsonnet J, Friedman GD, Vandersteen DP, Chang Y, Vogelman JH, Orentreich N, Sibley RK: Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med 325: 1127–1131, 1991Google Scholar
  2. 2.
    Wyatt JI, Rathbone BJ: Immune response of the gastric mucosa to Campylobacter pylori. Scand J Gastroenterol Suppl 142: 44–49, 1988Google Scholar
  3. 3.
    Wotherspoon AC, Ortiz-Hidalgo C, Falzon MR, Isaacson PG: Helicobacter pylori-associated gastritis and primary B-cell gastric lymphoma. Lancet 338: 1175–1176, 1991Google Scholar
  4. 4.
    Hussell T, Isaacson PG, Crabtree JE, Spencer J: The response of cells from low-grade B-cell gastric lymphomas of mucosa-associated lymphoid tissue to Helicobacter pylori. Lancet 342: 571–574, 1993Google Scholar
  5. 5.
    Wotherspoon AC, Doglioni C, Diss TC, Pan L, Moschini A, de Boni M, Isaacson PG: Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pylori. Lancet 342: 575–577, 1993Google Scholar
  6. 6.
    Cammarota G, Tursi A, Montalto M, Papa A, Branca G, Vecchio FM, Renzi C, Verzi A, Armuzzi A, Pretolani S, et al.: Prevention and treatment of low-grade B-cell primary gastric lymphoma by anti-H. pylori therapy. J Clin Gastroenterol 21: 118–122, 1995Google Scholar
  7. 7.
    Zhang T, O'Keefe SJ, Winter T, Marks IN, Ogden J: Effect of chronic duodenal ulceration and its treatment with lanzoprazole or sucralfate on gastroduodenal mucosal protein turnover and TGF-alpha, bFGF, and EGF receptor expression in humans. Dig Dis Sci 43: 2764–2770, 1998Google Scholar
  8. 8.
    Leung WK, To KF, Chan FK, Lee TL, Chung SC, Sung JJ: Interaction of Helicobacter pylori eradication and non-steroidal anti-inflammatory drugs on gastric epithelial apoptosis and proliferation: implications on ulcerogenesis. Aliment Pharmacol Ther 14: 879–885, 2000Google Scholar
  9. 9.
    Honig A, Witte F, Mirecka J, Binder C, Schauer A: Helicobacter pylori-induced hyperproliferation: relevance for gastric cancer development in connection with mutagenic factors. Anticancer Res 20: 1641–1648, 2000Google Scholar
  10. 10.
    Lynch DA, Mapstone NP, Clarke AM, Sobala GM, Jackson P, Morrison L, Dixon MF, Quirke P, Axon AT: Cell proliferation in Helicobacter pylori associated gastritis and the effect of eradication therapy. Gut 36: 346–350, 1995Google Scholar
  11. 11.
    Lynch DA, Mapstone NP, Clarke AM, Jackson P, Moayyedi P, Dixon MF, Quirke P, Axon AT: Correlation between epithelial cell proliferation and histological grading in gastric mucosa.J Clin Pathol 52: 367–371, 1999Google Scholar
  12. 12.
    Safatle-Ribeiro AV, Ribeiro U, Jr., Clarke MR, Sakai P, Ishioka S, Garrido AB, Jr., Gama-Rodrigues J, Safatle NF, Reynolds JC: Relationship between persistence of Helicobacter pylori and dysplasia, intestinal metaplasia, atrophy, inflammation, and cell proliferation following partial gastrectomy. Dig Dis Sci 44: 243–252, 1999Google Scholar
  13. 13.
    Brenes F, Ruiz B, Correa P, Hunter F, Rhamakrishnan T, Fontham E, Shi TY: Helicobacter pylori causes hyperproliferation of the gastric epithelium: pre-and post-eradication indices of proliferating cell nuclear antigen. Am J Gastroenterol 88: 1870–1875, 1993Google Scholar
  14. 14.
    Tarnawski AS, Jones MK: The role of epidermal growth factor (EGF) and its receptor in mucosal protection, adaptation to injury, and ulcer healing: involvement of EGF-R signal transduction path-ways.J Clin Gastroenterol 27(Suppl 1): S12–S20, 1998Google Scholar
  15. 15.
    Wong BC, Wang WP, So WH, Shin VY, Wong WM, Fung FM, Liu ES, Hiu WM, Lam SK, Cho CH: Epidermal growth factor and its receptor in chronic active gastritis and gastroduodenal ulcer before and after Helicobacter pylori eradication. Aliment Pharmacol Ther 15: 1459–1465, 2001Google Scholar
  16. 16.
    Konturek PC, Ernst H, Konturek SJ, Bobrzynski AJ, Faller G, Klingler C, Hahn EG: Mucosal expression and luminal release of epidermal and transforming growth factors in patients with duodenal ulcer before and after eradication of Helicobacter pylori. Gut 40: 463–469, 1997Google Scholar
  17. 17.
    Konturek PC, Bobrzynski A, Konturek SJ, Bielanski W, Faller G, Kirchner T, Hahn EG: Epidermal growth factor and transforming growth factor alpha in duodenal ulcer and non-ulcer dyspepsia patients before and after Helicobacter pylori eradication. Scand J Gastroenterol 33: 143–151, 1998Google Scholar
  18. 18.
    Schiemann U, Konturek J, Assert R, Rembiasz K, Domschke W, Konturek S, Pfeiffer A: mRNA expression of EGF receptor ligands in atrophic gastritis before and after Helicobacter pylori eradication. Med Sci Monit 8: CR53–CR58, 2002Google Scholar
  19. 19.
    Schiemann U, Konturek JW, Osterhoff M, Assert R, Rembiasz K, Pfeiffer D, Schatz H, Domschke W, Pfeiffer A: Decreased expression of epidermal growth factor receptor and mRNA of its ligands in Helicobacter pylori-infected gastric mucosa. Scand J Gastroenterol 36: 23–31, 2001Google Scholar
  20. 20.
    Derynck R: Transforming growth factor-alpha. Mol Reprod Dev 27: 3–9, 1990Google Scholar
  21. 21.
    Cutry AF, Kinniburgh AJ, Krabak MJ, Hui SW, Wenner CE: Induction of c-fos and c-myc proto-oncogene expression by epidermal growth factor and transforming growth factor alpha is calcium-independent. J Biol Chem 264: 19700–51970, 1989Google Scholar
  22. 22.
    Konturek PC, Konturek SJ, Pierzchalski P, Bielanski W, Duda A, Marlicz K, Starzynska T, Hahn EG: Cancerogenesis in Helicobacter pylori infected stomach—Role of growth factors, apoptosis and cyclooxygenases. Med Sci Monit 7: 1092–107, 2001Google Scholar
  23. 23.
    O'Keefe SJ: Measurement of gastric and duodenal mucosal protein turnover in humans. Dig Dis Sci 40: 2465–2468, 1995Google Scholar
  24. 24.
    Bouteloup-Demange C, Boirie Y, Dechelotte P, Gachon P, Beaufrere B: Gut mucosal protein synthesis in fed and fasted humans. Am J Physiol 274: E541–E546, 1998Google Scholar
  25. 25.
    Watt PW, Lindsay Y, Scrimgeour CM, Chien PA, Gibson JN, Taylor DJ, Rennie MJ: Isolation of aminoacyl-tRNA and its labeling with stable-isotope tracers: Use in studies of human tissue protein synthesis. Proc Natl Acad Sci USA 88: 5892–5896, 1991Google Scholar
  26. 26.
    Matthews DE, Schwarz HP, Yang RD, Motil KJ, Young VR, Bier DM: Relationship of plasma leucine and alpha-ketoisocaproate during a L–[1–13 C]leucine infusion in man: a method for measuring human intracellular leucine tracer enrichment. Metabolism 31: 1105–1112, 1982Google Scholar
  27. 27.
    Rennie MJ, Smith K, Watt PW: Measurement of human tissue protein synthesis: an optimal approach. Am J Physiol 266: E298–E307, 1994Google Scholar
  28. 28.
    Bennet WM, O'Keefe SJ, Haymond MW: Comparison of precursor pools with leucine, alpha-ketoisocaproate, and phenylalanine tracers used to measure splanchnic protein synthesis in man. Metabolism 42: 691–695, 1993Google Scholar
  29. 29.
    Carbonnel F, Messing B, Darmaun D, Rimbert A, Rongier M, Rigal O, Koziet J, Thuillier F, Desjeux JF: Energy and protein metabolism in malnutrition due to nonneoplastic gastrointestinal diseases. Metabolism 44: 1110–1115, 1995Google Scholar
  30. 30.
    O'Keefe SJ, Lemmer ER, Ogden JM, Winter T: The influence of intravenous infusions of glucose and amino acids on pancreatic enzyme and mucosal protein synthesis in human subjects. J Parent Enteral Nutr 22: 253–258, 1998Google Scholar
  31. 31.
    Misiewicz JJ: The Sydney System: a new classification of gastritis. Introduction. J Gastroenterol Hepatol 6: 207–208, 1991Google Scholar
  32. 32.
    Louw JA, Zak J, Lucke W, Le Roux E, Jaskiewicz K, Winter T, Lastovica A, Marks IN: Triple therapy with sucralfate is as effective as triple therapy containing bismuth in eradicating Helicobacter pylori and reducing duodenal ulcer relapse rates. Scand J Gastroenterol Suppl 191: 28–31, 1992Google Scholar
  33. 33.
    Terano A, Shimada T, Hiraishi H: Sucralfate and Helicobacter pylori. J Gastroenterol 31(Suppl 9): 63–65, 1996Google Scholar
  34. 34.
    Tarnawski A, Tanoue K, Santos AM, Sarfeh IJ: Cellular and molecular mechanisms of gastric ulcer healing. Is the quality of mucosal scar affected by treatment? Scand J Gastroenterol Suppl 210: 9–14, 1995Google Scholar
  35. 35.
    Szabo S, Kusstatscher S, Sandor Z, Sakoulas G: Molecular and cellular basis of ulcer healing. Scand J Gastroenterol Suppl 208: 3–8, 1995Google Scholar
  36. 36.
    Patchett SE, Katelaris PH, Zhang ZW, Alstead EM, Domizio P, Farthing MJ: Ornithine decarboxylase activity is a marker of premalignancy in longstanding Helicobacter pylori infection. Gut 39: 807–810, 1996Google Scholar
  37. 37.
    Preston-Martin S, Pike MC, Ross RK, Jones PA, Henderson BE: Increased cell division as a cause of human cancer. Cancer Res 50: 7415–7421, 1990Google Scholar
  38. 38.
    Moss SF, Calam J, Agarwal B, Wang S, Holt PR: Induction of gastric epithelial apoptosis by Helicobacter pylori. Gut 38: 498–501, 1996Google Scholar
  39. 39.
    Wagner S, Beil W, Westermann J, Logan RP, Bock CT, Trautwein C, Bleck JS, Manns MP: Regulation of gastric epithelial cell growth by Helicobacter pylori: Evidence for a major role of apoptosis. Gastroenterology 113: 1836–1847, 1997Google Scholar
  40. 40.
    Shibata J, Goto H, Arisawa T, Niwa Y, Hayakawa T, Nakayama A, Mori N: Regulation of tumour necrosis factor (TNF) induced apoptosis by soluble TNF receptors in Helicobacter pylori infection. Gut 45: 24–31, 1999Google Scholar

Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Trevor A. Winter
  • Mark Kidd
  • Philip Kaye
  • I. N. (Solly) Marks

There are no affiliations available

Personalised recommendations