Digestive Diseases and Sciences

, Volume 49, Issue 4, pp 579–589 | Cite as

In Vivo Effects of Bifidobacteria and Lactoferrin on Gut Endotoxin Concentration and Mucosal Immunity in Balb/c Mice

  • Elizabeth A. Griffiths
  • Linda C. Duffy
  • Floyd L. Schanbacher
  • Haiping Qiao
  • Diane Dryja
  • Allen Leavens
  • Jon Rossman
  • Gary Rich
  • Douglas Dirienzo
  • Pearay L. Ogra


The aim of the present study was to examine the effects of oral supplementation of newborn Balb/c mice with bifidobacteria (B. infantis, B. bifidum) and iron-free apo-lactoferrin (bovine, human) on gut endotoxin concentration and mucosal immunity. Endotoxin concentration was measured in ileocecal filtrates at 7, 14, 21, and 28 days postdelivery by a quantitative limulus amebocyte lysate test. While endotoxin levels in bifidobacteria-fed mice showed a steady rise over time, they were consistently lower than that observed in control animals. Results of lactoferrin supplementation varied depending on the specific time point, but overall by day 28, all treatment groups showed lower intestinal endotoxin concentrations compared to saline fed animals. Neither bifidobacteria nor lactoferrin stimulated an increase in B or T cells, or in cytokine production (IL-6, TNF-α, INF-γ), in Peyer's patches as measured by flow cytometry. Bifidobacteria and lactoferrin were well tolerated as dietary supplements and showed promising potential to reduce gut endotoxin levels.

bifidobacteria lactoferrin probiotics endotoxin mucosal immunity 


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Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Elizabeth A. Griffiths
    • 1
  • Linda C. Duffy
    • 1
  • Floyd L. Schanbacher
    • 2
  • Haiping Qiao
    • 1
  • Diane Dryja
    • 1
  • Allen Leavens
    • 1
  • Jon Rossman
    • 1
  • Gary Rich
    • 1
  • Douglas Dirienzo
    • 3
  • Pearay L. Ogra
    • 1
  1. 1.Department of Pediatrics, The Women and Children's Hospital of Buffalo, Kaleida Health, and School of MedicineState University of New York at BuffaloBuffalo
  2. 2.Department of Animal Sciences, Laboratories of Molecular and Developmental Biology, Ohio Agricultural Research and Development CenterOhio State UniversityWooster
  3. 3.National Dairy CouncilDairy Management Inc.RosemontUSA

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