Clinical & Experimental Metastasis

, Volume 21, Issue 3, pp 185–189 | Cite as

ICAM-1 supports adhesion of human small-cell lung carcinoma to endothelial cells

  • Angela H. Finzel
  • Armin J. Reininger
  • Patrick A. Bode
  • Laurenz J. Wurzinger

Abstract

Adhesion of tumor cells to endothelium via cell-adhesion molecules constitutes a crucial step in metastasis, which is largely responsible for the poor prognosis of small-cell lung carcinoma (SCLC). Patients with SCLC were reported to have elevated levels of intercellular adhesion molecule-1 (ICAM-1). The present study therefore focuses on endothelial ICAM-1 in tumor-cell adhesion. We found that the adherence of SCLC cells (cell lines H24, H69, H82) to cultured vascular endothelium in stasis and flow depends on the expression of ICAM-1. After blocking endothelial ICAM-1 with monoclonal antibodies, adhesion was significantly reduced. These results pinpoint ICAM-1 for the first time as a molecule crucially involved in SCLC cell-endothelial adhesion.

Abbreviations: ECGS – endothelial cell growth supplement; FCS – fetal calf serum; HUVECs – human umbilical vein endothelial cells; ICAM-1 – intercellular adhesion molecule-1; IL-1β– Interleukin-1β; SCLC – small-cell lung carcinoma

adhesion endothelium flow ICAM-1 small-cell lung carcinoma 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Angela H. Finzel
  • Armin J. Reininger
  • Patrick A. Bode
  • Laurenz J. Wurzinger

There are no affiliations available

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