Chromosome Research

, Volume 11, Issue 8, pp 749–757 | Cite as

Precise identification of chicken chromosomes in the lampbrush form using chromosome painting probes

  • Svetlana Derjusheva
  • Anna Kurganova
  • Alla Krasikova
  • Alsu Saifitdinova
  • Felix A. Habermann
  • Elena Gaginskaya

Abstract

Chromosome painting probes specific for macrochromosomes 1, 2, 3, 4, 5, and Z were applied to both mitotic and lampbrush chromosomes of the chicken (Gallus gallus domesticus). Five autosomal macrobivalents and sex chromosome Z in the lampbrush phase were identified and their correspondence to the target chromosomes in the metaphase of mitosis was shown. Nascent transcripts on lateral loops of the target lampbrush chromosome were intensively labelled when the hybridization was performed without RNase A treatment according to the DNA/(DNA+RNA) hybridization protocol.

chicken chromosome painting Gallus lampbrush chromosome RNP matrix 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Svetlana Derjusheva
    • 1
  • Anna Kurganova
    • 1
  • Alla Krasikova
    • 1
  • Alsu Saifitdinova
    • 1
  • Felix A. Habermann
    • 2
  • Elena Gaginskaya
    • 1
  1. 1.Oranienbaumskoie sch. 2Biological Institute of St. Petersburg UniversitySt. PetersburgRussia
  2. 2.Technical University of MunichFreising-WeihenstephanGermany

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