Cellular and Molecular Neurobiology

, Volume 24, Issue 4, pp 517–533 | Cite as

Processing, Distribution, and Function of VGF, a Neuronal and Endocrine Peptide Precursor

  • Andrea Levi
  • Gian-Luca Ferri
  • Elizabeth Watson
  • Roberta Possenti
  • Stephen R. J. Salton
Article

Abstract

1. The vgf gene encodes a neuropeptide precursor with a restricted pattern of expression that is limited to a subset of neurons in the central and peripheral nervous systems and to specific populations of endocrine cells in the adenohypophysis, adrenal medulla, gastrointestinal tract, and pancreas. In responsive neurons, vgf transcription is upregulated by neurotrophins, the basis for the original identification of VGF as nerve growth factor- (NGF) inducible in PC12 cells (A. Levi, J. D. Eldridge, and B. M. Paterson, Science229:393–395, 1985).

2. In this review, we shall summarize data concerning the transcriptional regulation of vgf in vitro, the structural organization of the vgf promoter as well as the transcription factors which regulate its activity.

3. On the basis of in situ hybridization and immunohistochemical studies, the in vivo tissue-specific expression of VGF during differentiation and in the adult will be summarized.

4. Parallel biochemical data will be reviewed, addressing the proteolytical processing of the pro-VGF precursor within the secretory compartment of neuroendocrine cells.

5. Finally, analysis of the phenotype of VGF knockout mice will be discussed, implying a nonredundant role of VGF products in the regulation of energy storage and expenditure.

VGF neurotrophin PC12 endocrine feeding metabolism NGF BDNF 

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Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Andrea Levi
    • 1
  • Gian-Luca Ferri
    • 2
  • Elizabeth Watson
    • 3
  • Roberta Possenti
    • 4
  • Stephen R. J. Salton
    • 3
  1. 1.Department of NeuroscienceUniversity of Tor VergataRomeItaly
  2. 2.Neuro-&-Endocrine Research, Department of CytomorphologyUniversity of Cagliari at MonserratoMonserratoCagliari, Italy
  3. 3.Fishberg Research Center for NeurobiologyMount Sinai School of MedicineNew YorkNew York
  4. 4.Institute of NeurobiologyNational Research CouncilRomeItaly

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