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Cellulose

, Volume 11, Issue 3–4, pp 301–312 | Cite as

Identification and expression analysis of genes encoding putative cellulose synthases (CesA) in the hybrid aspen, Populus tremula (L.) × P. tremuloides (Michx.)

  • Soraya Djerbi
  • Henrik Aspeborg
  • Peter Nilsson
  • Björn Sundberg
  • Ewa Mellerowicz
  • Kristina Blomqvist
  • Tuula T. TeeriEmail author
Article

Abstract

Cellulose is synthesized in plant cell walls by large membrane-bound protein complexes proposed to contain several copies of the catalytic subunit of the cellulose synthase, CesA. Here we report identification of 10 distinct CesA genes within a database of 100,000 ESTs of the hybrid aspen, Populus tremula (L.) × P. tremuloides (Michx.). Expression analyses in normal wood undergoing xylogenesis and in tension wood indicate xylem specific expression of four putative CesA isoenzymes, PttCesA1, PttCesA3-1, PttCesA3-2 and PttCesA9. Both the protein sequences and the expression profiles of PttCesA3-1 and PttCesA3-2 are very similar, and they may thus represent redundant copies of an enzyme with essentially the same function. Further, one of the generally more constitutively expressed CesA genes, PttCesA2, seems to be activated on the opposite side of a tension wood induced stem, while PttCesA6 appears to be more specific for leaf tissues. The rest of the hybrid aspen CesA genes were found to be relatively evenly expressed over the poplar tissues hereby studied.

Cellulose synthesis Expression profiling Hybrid aspen Plant cell wall Tension wood 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Soraya Djerbi
    • 1
  • Henrik Aspeborg
    • 1
  • Peter Nilsson
    • 1
  • Björn Sundberg
    • 2
  • Ewa Mellerowicz
    • 2
  • Kristina Blomqvist
    • 1
    • 3
  • Tuula T. Teeri
    • 1
    Email author
  1. 1.Department of Biotechnology, Royal Institute of TechnologyAlbaNova University CenterStockholmSweden
  2. 2.Department of Forest Genetics and Plant PhysiologyUme× Plant Science Center, SLUUme×Sweden
  3. 3.Department of Plant Biology and Forest GeneticsSwedish University of Agricultural Sciences (SLU), Genetic CenterUppsalaSweden

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