Biomedical Microdevices

, Volume 6, Issue 1, pp 33–40

Self-Assembled Coatings on Individual Monodisperse Magnetite Nanoparticles for Efficient Intracellular Uptake

  • Yong Zhang
  • Conroy Sun
  • Nathan Kohler
  • Miqin Zhang


Monodispersed iron oxide superparamagnetic nanoparticles were prepared using a novel circulating system. A simple dialysis method was developed to immobilize nanoparticles with functional biopolymers and targeting agents, which avoids the use of the normal centrifugation process that may cause particle agglomeration during the coating process. To enhance the specific targeting capability of the nanoparticles, a new chemical scheme was introduced, in which folic acid (FA) was chosen as the targeting agent combined with PEG serving to improve biocompatibility of nanoparticles. The AFM characterization showed that the nanoparticles produced are well dispersed with a narrow size distribution. The FTIR and XPS spectrum analyses indicated that PEG and FA-PEG have been chemically/covalently bonded to nanoparticles using synthetic approach introduced in this study. Our biological study showed that coating nanoparticles with PEG-FA significantly enhanced the intracellular uptake of nanoparticles by target cells.

Monodisperse magnetite nanoparticles PEG PEG-FA intracellular uptake 


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  1. 1.
    P. Reimer, R. Weissleder, Radiologe 36, 153 (1996).Google Scholar
  2. 2.
    C.W. Jung, P. Jacobs, Res. Imaging 13, 661 (1995).Google Scholar
  3. 3.
    P.K. Gupta, C.T. Hung, Life Sci. 44, 175 (1989).Google Scholar
  4. 4.
    A.S. Lubbe, C. Bergemann, W. Huhnt, T. Fricke, H. Riess, J.W. Brock, and D. Huhn, Cancer Res. 56, 4694 (1996).Google Scholar
  5. 5.
    D.C.F. Chan, D.B. Kirpotin, and P.A. Bunn, J. Magn. Magn. Mat. 122, 374 (1993).Google Scholar
  6. 6.
    A. Jordan, P. Wust, and R. Scholz, In: Scientific and Clinical Applications of Magnetic Carriers, edited by U. Häfeli, W. Schütt, J. Teller, and M. Zborowski (Plenum Press, New York, 1997), p. 569.Google Scholar
  7. 7.
    R.S. Molday and D. Mackenzie, J. Immunol. Meth. 52, 353 (1982).Google Scholar
  8. 8.
    C. Grüttner, J. Teller, and W. Schütt, In: Scientific and Clinical Applications of Magnetic Carriers, edited by U. Häfeli, W. Schütt, J. Teller, and M. Zborowski (Plenum Press, New York, 1997), p. 53.Google Scholar
  9. 9.
    E. Allemann, R. Gurny, and E. Doelker, Eur. J. Pharm. Biopharm. 39, 173 (1993).Google Scholar
  10. 10.
    I. Brigger, J. Morizet, G. Aubert, H. Chacun, M.J. Terrier-Lacombe, P. Couvreur, and G. Vassal, J. Pharmacol. Exp. Ther. 303, 928 (2002).Google Scholar
  11. 11.
    Y.F. Lam and G. Kotowycz, Can. J. Chem. 50, 2357 (1972).Google Scholar
  12. 12.
    M. Khaled, K. Abu, and L. Carlos, Biochem. Biophys. Res. Commun. 130, 1273 (1985).Google Scholar
  13. 13.
    Y. Zhang, N. Kohler, and M.Q. Zhang, Biomaterials 23, 1553 (2002).Google Scholar
  14. 14.
    Y. Lu and P.S. Low, Advanced Drug Delivery Reviews 54, 675 (2002).Google Scholar
  15. 15.
    J.A. Reddy and P.S. Low, Crit. Rev. Ther. Drug Carrier Syst. 15, 587 (1998).Google Scholar
  16. 16.
    M.O. Oyewumi and R.J. Mumper, Bioconjugate Chem. 13, 1328 (2002).Google Scholar
  17. 17.
    W.J. Guo and R.J. Lee, AAPS PHARMSCI, 1, art. no. 19 (1999).Google Scholar
  18. 18.
    T. Prozorov, R. Prozorov, and A. Gedanken, Adv. Mater. 18, 1529 (1998).Google Scholar
  19. 19.
    D.K. Kim, Y. Zhang, W. Voit, K.V. Rao, and M. Muhammed, J. Magn. Magn. Mater. 225, 30 (2001).Google Scholar
  20. 20.
    E. Tronc, P. Belleville, J.P. Jolivet, and J. Livage, Langmuir 8, 313 (1992).Google Scholar
  21. 21.
    K. Nakatsuka and B. Jeyadevan, IEEE Trans. Magn. Magn. 30, 4671 (1994).Google Scholar
  22. 22.
    K.J. Davies, S. Wells, and S.W. Charles, J. Magn. Magn. Mater. 122, 24 (1993).Google Scholar
  23. 23.
    K.J. Davies, S. Wells, R.V. Upadhyay, S.W. Charles, K. Ogrady, M. Elhilo, T. Meaz, and S. Morup, J. Magn. Magn. Mater. 149, 14 (1995).Google Scholar
  24. 24.
    D.K. Kim, Y. Zhang, W. Voit, K.V. Rao, J. Kehr, B. Bjelke, and M. Muhammed, Scripta mater. 44, 1713 (2001).Google Scholar
  25. 25.
    C.G. Granqvist and R.H. Buhrman, J. Appl. Phys. 47, 2200 (1976).Google Scholar
  26. 26.
    M.D. Butterworth, L. Illum, and S.S. Davis, Physicochemical and Engineering Aspects 179, 93 (2001).Google Scholar
  27. 27.
    N.S. McIntyre and D.G. Zetaruk, Anal. Chem. 49, 1519 (1977).Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Yong Zhang
    • 1
  • Conroy Sun
    • 1
  • Nathan Kohler
    • 1
  • Miqin Zhang
    • 1
  1. 1.Department of Materials Science and EngineeringUniversity of Washington

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