Biochemistry (Moscow)

, Volume 69, Issue 4, pp 413–419

Inhibition of Gap Junction Intercellular Communications in Cell Culture by Polycyclic Aromatic Hydrocarbons (PAH) in the Absence of PAH Metabolism

  • Ju. Ju. Sharovskaja
  • A. V. Vaiman
  • N. A. Solomatina
  • V. A. Kobliakov
Article

Abstract

We have studied the effect of polycyclic aromatic hydrocarbons (PAH) on gap junction intercellular communications (GJIC) in culture of hepatoma cells Hep G2 and G27. Carcinogenic PAH inhibited GJIC in both cultures in contrast to non-carcinogenic PAH. We showed that both constitutive and inducible expressions of mRNAs of Ah receptor and cytochrome P4501A1 (the main isoform involved in PAH metabolism) were absent in hepatoma G27 cells. We concluded that the initial, non-metabolized molecules of carcinogenic PAH are responsible for changes in GJIC through interaction with an unknown factor in the cellular membrane.

gap junction intercellular communications polycyclic aromatic hydrocarbons Ah receptor 

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Copyright information

© MAIK “Nauka/Interperiodica” 2004

Authors and Affiliations

  • Ju. Ju. Sharovskaja
    • 1
  • A. V. Vaiman
    • 2
  • N. A. Solomatina
    • 2
  • V. A. Kobliakov
    • 2
  1. 1.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Institute of Carcinogenesis, Blokhin Russian Cancer Research CenterRussian Academy of Medical SciencesMoscowRussia

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