Biologia Plantarum

, Volume 47, Issue 4, pp 561–568 | Cite as

Structural Changes in Radish Seedlings Exposed to Cadmium

  • A.P. Vitória
  • A.P.M. Rodriguez
  • M. Cunha
  • P.J. Lea
  • R.A. Azevedo
Article

Abstract

Radish (Raphanus sativus L. cv. Redondo Vermelho) seedlings were analysed by light and scanning electron microscopy to characterize the structural changes caused by the exposure to 0.5 or 1.0 mM cadmium chloride for 24, 48 and 72 h. The analyses showed changes in the anatomical and morphological characteristics of roots, stems and leaves of two-week-old seedlings. In all tissues, pressure potential was decreased. Premature death with the disintegration of the epidermis and an increase in the number of root hairs was observed in roots exposed to Cd. The stem of seedlings exposed to Cd exhibited more cells layers in the cambial region. The main effects observed in leaves in response to Cd were stomatal closure, lack of cell wall thickening and alterations in the shape of the chloroplasts. It is suggested that the structural changes observed in seedlings treated with Cd were mainly caused by a Cd-induced decrease in water uptake.

cell wall heavy metals Raphanus salivus stomata 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • A.P. Vitória
    • 1
  • A.P.M. Rodriguez
    • 1
  • M. Cunha
    • 2
  • P.J. Lea
    • 3
  • R.A. Azevedo
  1. 1.Laboratório de Melhoramento Genético Vegetal, Centro de Ciências e Tecnologias AgropecuáriasUniversidade Estadual do Norte Fluminense, Campos dos Goytacazes, CEPRJBrazil, e-mail
  2. 2.Laboratório de Biologia Celular e Tecidual, Centro de Biociências e BiotecnologiaUniversidade Estadual do Norte Fluminense, Campos dos Goytacazes, CEPRJBrazil
  3. 3.Department of Biological SciencesUniversity of LancasterLancaster LA1 4YQUK

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