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Biologia Plantarum

, Volume 47, Issue 4, pp 527–533 | Cite as

Activities of Antioxidant Systems During Germination of Chenopodium rubrum Seeds

  • T. Dučić
  • I. Lirić-rajlić
  • A. Mitrović
  • K. Radotić
Article

Abstract

The activities of superoxide-dismutase (SOD), catalase (CAT) and peroxidase (POD), and concentrations of glutathione and ascorbate have been studied during the first stages of germination in Chenopodium rubrum L. seeds. The highest CAT and SOD activity was found prior to radicle protrusion, while POD activity was maximal at the time of radicle protrusion and seedling development, new POD isozymes simultaneously appearing. The concentrations of total, reduced and oxidized glutathione showed similar changes during germination, the highest values being detected at the time of radicle protrusion. Ascorbic acid was present in the seeds in a detectable concentration only at the time preceding radicle protrusion, while its oxidized form dehydroascorbic acid was detected during the whole germination period studied. Gibberellic acid (GA3, 160 μM) had no effect on germination percentage, but in presence of GA3, SOD and CAT activity notably increased prior to radicle protrusion, and oxidized glutathione concentration decreased in further germination.

ascorbic acid catalase gibberellic acid glutathione peroxidase reactive oxygen species superoxide-dismutase 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • T. Dučić
    • 1
  • I. Lirić-rajlić
    • 1
  • A. Mitrović
    • 1
  • K. Radotić
    • 1
  1. 1.Center for Multidisciplinary StudiesUniversity of Belgrade, 29. Novembar 142BelgradeSerbia and Montenegro

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