Abstract
In vitro regeneration of four Begonia genotypes, B. semperflorens, B. rex, B.×elatior, and hybrid of Begonia with unknown parents ‘Tiger’ was carried out starting from leaf and petiole segments as explants. Five Murashige and Skoog's derived media were tested, three of them supplemented with α-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA), and the other two with NAA and kinetin (KIN) in different concentrations. Shoot regeneration was preferentially induced on the BA containing media, quantitative differences being observed among explants and genotypes.
References
Babber, S., Mittal, K., Ahlawat, R., Varghese, T.M.: Micropropagation of Cardiospermum halicacabum.-Biol. Plant. 44: 603-606, 2001.
Burrit, D.J., Leung, D.W.M.: Organogenesis in cultured petiole explants of Begonia x erythrophylla: the timing and specificity of the inductive stimuli.-J. exp. Bot. 47: 557-567, 1996.
Cassells, A.C., Morrish, F.M.: Variation in adventitious regenerants of Begonia rex Putz. ‘Lucille Closon’ as a consequence of cell ontogeny, callus ageing and frequency of callus sub-culture.-Scientia Hort. 32: 135-143, 1987.
Chlyah, A., Tran Thanh Van, M.: Differential reactivity in epidermal cells of Begonia rex excised and grown in vitro.-Physiol. Plant. 35: 16-20, 1975.
Khoder, M., Villemur, P., Jonard, R.: Vegetative propagation of the Elatior begonia cv. Rieger from different organs grown in vitro.-Compt. rend. Acad. Sci. Paris Sêr. III 293: 403-408, 1981.
Kishimoto, S., Aida, R., Shibata, M.: Agrobacterium tumefaciens-mediated transformation of Elatior Begonia (Begonia x hiemalis Fotsch).-Plant Sci. 162: 697-703, 2002.
Kiyokawa, T., Kikuchi, Y., Kamada, H., Harada, H.: Genetic transformation of Begonia tuberhybrida by Ri rol genes.-Plant Cell Rep. 15: 606-609, 1996.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962.
Saritha, K.V., Prakash, E., Ramamurthy, N., Naidu, C.V.: Micropropagation of Spilanthes acmella Murr.-Biol. Plant. 45: 581-584, 2002.
Simmonds, J.: Induction, growth and direct rooting of adventitious shoots of Begonia x hiemalis.-Plant Cell Tissue Organ Cult. 3: 283-289, 1984.
Takayama, S.: Begonia.-In: Amirato, P.V., Evans, D.A., Sharp, W.R., Bajaj, Y.P.S. (ed.): Handbook of Plant Cell Culture. Vol. 5. Pp. 253-282. McGraw-Hill Publ. Co., New York 1990.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Espino, F., Linacero, R., Rueda, J. et al. Shoot Regeneration in Four Begonia Genotypes. Biologia Plantarum 48, 101–104 (2004). https://doi.org/10.1023/B:BIOP.0000024282.01087.a3
Issue Date:
DOI: https://doi.org/10.1023/B:BIOP.0000024282.01087.a3