Biologia Plantarum

, Volume 47, Issue 2, pp 179–183 | Cite as

Antioxidant Enzyme Activities during in vitro Morphogenesis of Gladiolus and the Effect of Application of Antioxidants on Plant Regeneration

  • S. Dutta Gupta
  • S. Datta


Activity of antioxidant enzymes was evaluated during somatic embryogenesis and shoot organogenesis from cultured leaf segments of Gladiolus hybridus Hort. The effect of exogenous antioxidants on somatic embryogenesis and shoot organogenesis has also been monitored. Activity of superoxide dismutase (SOD) gradually increased during somatic embryogenesis. while activities of catalase (CAT) and peroxidase (POX) decreased. In contrast, increase in CAT and POX activity and a concomitant decrease in SOD activity were noted during shoot organogenesis. Exogenous application of antioxidants such as glutathione (GSH), α-tocopherol and ascorbate (AA) inhibited somatic embryogenesis but stimulated shoot organogenesis. The frequency of somatic embryogenesis increased with the addition of H2O2. However, H2O2 inhibited shoot organogenesis.

catalase Gladiolus hybridus peroxidase reactive oxygen species shoot organogenesis somatic embryogenesis superoxide dismutase 


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© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.Department of Agricultural and Food EngineeringIndian Institute of TechnologyKharagpurIndia

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