Biotechnology Letters

, Volume 26, Issue 20, pp 1593–1594

Improved long-term cryostorage of Escherichia colicompetent cells using trehalose

  • Taeho Ahn
  • Seong Su Kang
  • Chul-Ho Yun


Trehalose added to storage buffers for chemical-induced transformations at 200 mm increased the survival of Escherichia coli competent cells 1.3∼4.8-fold over 180 d cryostorage compared with dimethyl sulfoxide or glycerol.

competent Escherichia coli transformation trehalose 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Taeho Ahn
    • 1
  • Seong Su Kang
    • 2
  • Chul-Ho Yun
    • 3
  1. 1.Department of BiochemistryCollege of Veterinary MedicineKorea
  2. 2.Interdisciplinary Program of Brain ScienceChonnam National UniversityGwangjuKorea
  3. 3.School of Biological Sciences and TechnologyChonnam National UniversityGwangjuKorea

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