Biotechnology Letters

, Volume 26, Issue 13, pp 1057–1059 | Cite as

Scale-up production of puerarin from hairy roots of Pueraria phaseoloides in an airlift bioreactor

  • Spiridon Kintzios
  • Olga Makri
  • Eleni Pistola
  • Theodoros Matakiadis
  • He Ping Shi
  • Athanassios Economou
Article

Abstract

Hairy roots of the Chinese herb, Pueraria phaseoloides, obtained from leaf explants and transformed with the Agrobacterium rhizogenes, were cultured in 2.5 l airlift bioreactors for three weeks. Puerarin accumulated at 5570 μg g−1 dry wt, which is near 200 times as much as in 250 ml flask cultures. In addition, puearin was exuded into the nutrient medium at final concentrations higher than in the hairy roots themselves.

airlift bioreactor Pueraria phaseoloides puerarin transformed roots 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Spiridon Kintzios
    • 1
  • Olga Makri
    • 1
  • Eleni Pistola
    • 1
  • Theodoros Matakiadis
    • 1
  • He Ping Shi
    • 2
  • Athanassios Economou
    • 3
  1. 1.Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology, AgriculturalUniversity of AthensAthensGreece
  2. 2.College of Life ScienceSouth China Normal UniversityGuangzhouP.R. China
  3. 3.Laboratory of FloricultureAristotle UniversityThessalonikiGreece

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