Biotechnology Letters

, Volume 26, Issue 12, pp 1013–1017

Improvement of recombinant hirudin production by controlling NH4+ concentration in Pichia pastoris fermentation

  • Jizhong Yang
  • Xiangshan Zhou
  • Yuanxing Zhang
Article

DOI: 10.1023/B:BILE.0000030049.75092.95

Cite this article as:
Yang, J., Zhou, X. & Zhang, Y. Biotechnology Letters (2004) 26: 1013. doi:10.1023/B:BILE.0000030049.75092.95

Abstract

In recombinant Pichia pastoris fermentation for hirudin production in a 5 l fermenter, a new strategy was explored to match the short fermentation time at low NH4+ concentration with decreased hirudin degradation at high NH4+ concentration. A combination of a defined medium containing initial 0.025 m NH4+ with NH4+ addition up to 0.6 m in the growth phase was achieved in both the improvement of hirudin production and the repression of hirudin degradation. Intact and total hirudin reached 2.63 g l−1 and 4.25 g l−1, respectively.

fermentation hirudin NH4+ Pichia pastoris proteolytic degradation 

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Jizhong Yang
    • 1
    • 2
  • Xiangshan Zhou
    • 1
  • Yuanxing Zhang
    • 1
  1. 1.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiP.R. China
  2. 2.Dong E E Jiao Ehua Biopharmaceutical CompanyShandong ProvinceP.R. China

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