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Biotechnology Letters

, Volume 26, Issue 10, pp 813–817 | Cite as

Cloning of two carotenoid ketolase genes from Nostoc punctiforme for the heterologous production of canthaxanthin and astaxanthin

  • Sabine Steiger
  • Gerhard Sandmann
Article

Abstract

For the heterologous synthesis of keto-carotenoids such as astaxanthin, two carotenoid ketolase genes crtW38 and crtW148, were cloned from the cyanobacterium, Nostoc punctiforme PCC 73102 and functionally characterized. Upon expression in Escherichia coli, both genes mediated the conversion of β-carotene to canthaxanthin. However in a zeaxanthin-producing E. coli, only the gene product of crtW148 introduced 4-keto groups into the 3,3′-dihydroxy carotenoid zeaxanthin yielding astaxanthin. The gene product of crtW38 was unable to catalyze this reaction. Both ketolases differ in their interaction with a hydroxylase in the biosynthetic pathway from β-carotene to astaxanthin.

astaxanthin canthaxanthin β-carotene ketolase heterologous carotenoid production 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Sabine Steiger
    • 1
  • Gerhard Sandmann
    • 1
  1. 1.Biosynthesis Group, Botanical InstituteGoethe UniversityFrankfurtGermany

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