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Biotechnology Letters

, Volume 26, Issue 6, pp 525–528 | Cite as

Michael addition of imidazole with acrylates catalyzed by alkaline protease from Bacillus subtilis in organic media

  • Ying Cai
  • Shi-Ping Yao
  • Qi Wu
  • Xian-Fu Lin
Article

Abstract

A new activity of alkaline protease from Bacillus subtilis for Michael addition reactions of imidazole, 4-nitro-1H-imidazole and 2-methyl-4-nitro-1H-imidazole with acrylates and acrylic acid was investigated. The reactions were carried out in pyridine at 50 °C for 72 h. Five N-substituted imidazole derivatives were obtained using acrylate esters, but not acrylic acid, in yields from 62% to 76%.

alkaline protease enzymatic synthesis imidazole Michael addition organic media 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Ying Cai
    • 1
  • Shi-Ping Yao
    • 1
  • Qi Wu
    • 1
  • Xian-Fu Lin
    • 1
  1. 1.Department of ChemistryZhejiang UniversityHangzhouP.R. China

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