Biotechnology Letters

, Volume 26, Issue 1, pp 41–44 | Cite as

Quantitative characterisation of the morphology of Trichoderma harzianum cultured in shake-flasks and containing Tween 40

  • Savidra Lucatero
  • Enrique Galindo
  • C. Patricia Larralde-Corona
Article

Abstract

Image analysis was used to measure the effect of Tween 40 on the morphology of Trichoderma harzianum. The percentage of pellets was maximal (93%) with Tween at 0.2 ml l−1, whereas the maximal proportion of dispersed mycelia (40%) was with Tween at 1.6 ml l−1. The particle median diameter decreased from 2 to 0.5 mm as Tween concentration was increased and was inversely correlated to the biomass dry weight. Adding pre-determined aliquots of Tween 40 to the culture medium can be used to define the morphological characteristics of Trichoderma harzianum in submerged shake-flask culture.

fungi morphology shake-flasks Trichoderma Tween 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Savidra Lucatero
    • 1
  • Enrique Galindo
    • 1
  • C. Patricia Larralde-Corona
  1. 1.Instituto de Biotecnología-UNAMCuernavaca, MorelosMéxico

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