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Biotechnology Letters

, Volume 26, Issue 1, pp 15–19 | Cite as

Microbial degradation of poly(d-3-hydroxybutyrate) by a new thermophilic Streptomyces isolate

  • Buenaventurada P. Calabia
  • Yutaka Tokiwa
Article

Abstract

A new thermophilic microorganism capable of degrading poly(d-3-hydroxybutyrate) (PHB) was isolated from soil. A phylogenetic analysis based on 16S rDNA sequences indicated that the new isolate belongs to genus Streptomyces. PHB film and powder were completely degraded after 6 and 3 d cultivation, respectively at 50 °C. Scanning micrographs showed adherence of the microbial cells to the entire film surface, indicating that biodegradation occurs by colonization of the PHB surface. The film was degraded both by microbial attack and by the action of an extracellular enzyme secreted by the microorganism. The strain can also degrade poly(ethylene succinate), poly(ester carbonate), polycaprolactone and poly(butylene succinate), but to a lesser extent.

biodegradation microbial degradation poly(3-hydroxybutyrate) Streptomyces thermophilic 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Buenaventurada P. Calabia
    • 1
  • Yutaka Tokiwa
    • 2
  1. 1.Department of Science and TechnologyIndustrial Technology Development InstituteMetro ManilaPhilippines
  2. 2.National Institute of Advanced Industrial Science and Technology (AIST)Tsukuba Central 6Tsukuba, IbarakiJapan

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