Microbial degradation of poly(d-3-hydroxybutyrate) by a new thermophilic Streptomyces isolate
- 251 Downloads
A new thermophilic microorganism capable of degrading poly(d-3-hydroxybutyrate) (PHB) was isolated from soil. A phylogenetic analysis based on 16S rDNA sequences indicated that the new isolate belongs to genus Streptomyces. PHB film and powder were completely degraded after 6 and 3 d cultivation, respectively at 50 °C. Scanning micrographs showed adherence of the microbial cells to the entire film surface, indicating that biodegradation occurs by colonization of the PHB surface. The film was degraded both by microbial attack and by the action of an extracellular enzyme secreted by the microorganism. The strain can also degrade poly(ethylene succinate), poly(ester carbonate), polycaprolactone and poly(butylene succinate), but to a lesser extent.
Unable to display preview. Download preview PDF.
- Budwill KP, Fedorak M, Page WJ (1992) Methanogenic degradation of poly(3-hydroxyalkanoates). Appl. Environ. Microbiol. 58: 1398–1401.Google Scholar
- Delafield FP, Doudoroff M, Palleroni NJ, Lusty CJ, Cantopoulus R (1965) Decomposition of poly-hydroxybutyrate by pseudomonads. J. Bacteriol. 90: 1455–1466.Google Scholar
- Doi Y (1990) Microbial Polyesters. New York: VCH.Google Scholar
- Janssen PH, Harfoot CG (1996) Ilyobacter delafieldi sp. nov., a metabolically restricted anaerobic bacterium fermenting PHB. Arch. Microbiol. 154: 253–259.Google Scholar
- Jendrossek D, Knoke I, Habibian RB, Steinbuchel A, Schelegel HG (1993) Degradation of poly(3-hydroxybutyrate), PHB by bacteria and purification of novel PHB depolymerase from Comamonas sp. J. Environ. Polym. Degrad. 1: 53–63.Google Scholar
- Mergaert J, Webb V, Anderson C, Wouters A, Swings J (1993) Microbial degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in soil. Appl. Environ. Microbiol. 59: 3233–3238.Google Scholar
- Nishida H, Tokiwa Y (1993a) Distribution of poly(3-hydroxybutyrate) and poly(å-caprolactone) aerobic degrading microorganisms in different environments. J. Environ. Polym. Degrad. 1: 227–233.Google Scholar
- Nishida H, Tokiwa Y (1993b) Distribution of poly(3-hydroxybutyrate), degrading anaerobic bacteria. Jap. J. Polym. Sci.Tech. 50: 745–746.Google Scholar
- Oda Y, Asari H, Urakami T, Tonomora K (1995) Microbial degradation of poly(3-hydroxybutyrate) and polycaprolactone by filamentous fungi. J. Ferment. Bioeng. 80: 265–269.Google Scholar
- Pranamuda H, Tokiwa Y, Tanaka H (1997) Polylactide degradation by an Amycolatopsis sp. Appl. Environ. Microbiol. 63: 1637–1640.Google Scholar
- Takeda M, Koizumi J, Yabe K, Adachi K (1998) Thermostable poly(3-hydroxybutyrate) depolymerase of a thermophilic strain of Leptothrix sp. isolated from a hot spring. J. Ferment. Bioeng. 85: 375–380.Google Scholar
- Tansengco ML, Tokiwa Y (1998) Comparative population study of aliphatic polyesters-degrading microorganisms at 50 °C. Chem. Lett. 10: 1043–1044.Google Scholar