Biotechnology Letters

, Volume 25, Issue 24, pp 2079–2083

Methodology for using a universal primer to label amplified DNA segments for molecular analysis

Article

DOI: 10.1023/B:BILE.0000007075.24434.5e

Cite this article as:
Guo, D. & Milewicz, D.M. Biotechnology Letters (2003) 25: 2079. doi:10.1023/B:BILE.0000007075.24434.5e

Abstract

Detection of human DNA polymorphisms using high throughput methods often relies on generating a labeled DNA fragment which is generated by PCR using sequence-specific primers with an end labeled tag to detect a variant. The disadvantage of the synthesis of an end-labeled, sequence-specific primer to assay each DNA variant lies in the costs and time consume. In this report, we have demonstrated a methodology that can generate labeled DNA fragments using a labeled universal primer instead of requiring sequence-specific primers for each DNA variant.

biotin-labeled primer fluorescently labeled primer genotyping Pyrosequencing universal primer 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.Department of Internal MedicineUniversity of Texas – Houston Medical SchoolHoustonUSA

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