Methodology for using a universal primer to label amplified DNA segments for molecular analysis
- Cite this article as:
- Guo, D. & Milewicz, D.M. Biotechnology Letters (2003) 25: 2079. doi:10.1023/B:BILE.0000007075.24434.5e
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Detection of human DNA polymorphisms using high throughput methods often relies on generating a labeled DNA fragment which is generated by PCR using sequence-specific primers with an end labeled tag to detect a variant. The disadvantage of the synthesis of an end-labeled, sequence-specific primer to assay each DNA variant lies in the costs and time consume. In this report, we have demonstrated a methodology that can generate labeled DNA fragments using a labeled universal primer instead of requiring sequence-specific primers for each DNA variant.