Biotechnology Letters

, Volume 25, Issue 24, pp 2055–2059

Cellulose production by Gluconacetobacter hansenii in a medium containing ethanol



The addition of 1% (v/v) ethanol to the basal medium inhibited growth of Gluconacetobacter hansenii but decreased the numbers of non-cellulose producing cells. Cellulose production increased 1.7 times to approx. 2.5 g l−1 and showed a pattern of mixed growth-associated production. Microbial cells produced rigid pellicle-type bacterial cellulose as the shell of a large lump of bacterial cellulose like a static culture. The inoculum cultivated for 3 d maintained cellulose production by the fifth batch.

bacterial cellulose cellulose-negative mutant ethanol Gluconacetobacter hansenii 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Borzani W, de Souza SJ (1995) Mechanism of the film thickness increasing during the bacterial production of cellulose on nonagitated liquid media. Biotechnol. Lett. 17: 1271–1272.Google Scholar
  2. Delmer DP (1999) Cellulose biosynthesis: exciting times for a diffi-cult field of study. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50: 245–276.Google Scholar
  3. Jeong YJ, Lee IS (2000) A view of utilizing cellulose produced by Acetobacter bacteria. Food Ind. Nutr. 5: 25–29.Google Scholar
  4. Jung JY, Park YH, Park JK (2003) Effects of alcohols on the production of bacterial cellulose. Korean J. Biotechnol. Bioeng. 18: 127–132.Google Scholar
  5. Klemm D, Schumann D, Udhard U, Marsch S (2001) Bacterial synthesized cellulose–artficial blood vessels for microsurgery. Prog. Polym. Sci. 26: 1561–1603.Google Scholar
  6. Matthysse AG, Thomas DL, White AR (1995) Mechanism of cellulose synthesis in Agrobacterium tumefaciens. J. Bacteriol. 177: 1076–1081.Google Scholar
  7. Naritomi T, Kouda T, Yano H, Yoshinaga F (1998) Effect of ethanol on bacterial cellulose production from fructose in continuous culture. J. Ferment. Bioeng. 85: 598–603.Google Scholar
  8. Park JK, Park YH, Jung JY (2003) Production of bacterial cellulose by Gluconacetobacter hansenii isolated from rotten apple. Biotechnol. Bioprocess Eng. 8: 83–88.Google Scholar
  9. Schramm M, Hestrin S (1954) Factors affecting production of cellulose at the air/liquid interface of a culture of Acetobacter xylinum. J. Gen. Microbiol. 11: 123–129.Google Scholar
  10. Son HJ, Lee OM, Kim YG, Park YK, Lee SJ (2000) Characteristics of cellulose production by Acetobacter sp. A9 in static culture. Korean J. Biotechnol. Bioeng. 15: 573–577.Google Scholar
  11. Valla S, Kjosbakken J (1981) Cellulose-negative mutants of Acetobacter xylinum. J. Gen. Microbiol. 128: 1401–1408.Google Scholar
  12. Yamanaka S, Watanabe K, Kitamura N, Iguchi M, Mitsuhashi S, Nishi Y, Uryu M (1989) The structure and mechanical properties of sheets prepared from bacterial cellulose. J. Mater. Sci. 24: 3141–3145.Google Scholar
  13. Yoshino T, Asakura T, Toda K (1996) Cellulose production by Acetobacter pasteurianus on silicone membrane. J. Ferment. Bioeng. 81: 32–36.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Joong Kon Park
    • 1
  • Jae Yong Jung
    • 1
  • Youn Hee Park
    • 1
  1. 1.Department of Chemical EngineeringKyungpook National UniversityTaeguRepublic of Korea

Personalised recommendations