Gene Frequency Distribution of the BoLA-DRB3 Locus in Saavedreño Creole Dairy Cattle
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The objective of this study is to describe the gene frequency distribution of the bovine lymphocyte antigen (BoLA)-DRB3 locus in Saavedreño Creole dairy cattle and to compare it with previously reported patterns in other cattle breeds. One hundred and twenty-five Saavedreño Creole dairy cattle were genotyped for the BoLA-DRB3.2 allele by polymerase chain reaction and restriction fragment length polymorphism. Twenty-two out of 53 previously identified BoLA-DRB3.2 alleles were detected, with gene frequencies ranging from 0.4 to 16.8%. Seventy percent of the variation corresponded to the seven most frequent alleles (BoLA-DRB3.2*7, *8, *11, *16, *27, *36, and *37). The studied population exhibits a high degree of expected heterozygosity (he = 0.919). The FIS index did not show significant deviation from Hardy-Weinberg equilibrium. However, the neutrality test showed an even gene frequency distribution. This result could be better explained assuming balancing selection instead of neutral or positive selection for one or a few alleles. In conclusion, the results of this study demonstrated that BoLA-DRB3.2 is a highly polymorphic locus in Saavedreño Creole dairy cattle, with significant variation in allele frequency among cattle breeds.
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- Aravindakshan, T. V., and Nainar, A. M. (1999). Genetic polymorphism of the BoLA-DRB3 gene in Jersey crossbred and Ongole cattle revealed by PCR-RFLP. J. Anim. Sci. 69:424–427.Google Scholar
- Black, F. L., and Salzano, F. M. (1983). Evidence for heterosis in the HLA system. Am. J. Hum. Genet. 33:894–899.Google Scholar
- Boyce, W. M., Hedrick, P. W., Muggli-Cockett, N. E., Kalinowski, S., Penedo, M. C. T., and Ramey, R., II (1996). Genetic variation of major histocompatibility complex and microsatellite loci: A comparison in Bighorn sheep. Genetics 145:421–433.Google Scholar
- Golijow, C. D. (1996). Estudio de la reducción de la variabilidad genética por acción de la selección artificial en poblaciones de Bos taurus, PhD Thesis, Universidad Nacional de La Plata, Argentina.Google Scholar
- Hedrick, P. W. (1994). Evolutionary genetics of the major histocompatibility complex. Am. Nat. 143:945–964.Google Scholar
- Klein J. (1986). Natural History of the Major Histocompatibility Complex, Wiley, New York.Google Scholar
- Lewin, H. A. (1994). Host genetic mechanism of resistance and susceptibility to a bovine retroviral infection. Anim. Biotechnol. 5:183–191.Google Scholar
- Potts, W. K., Manning, C. J., and Wakeland, E. K. (1991). MHC genotype influences matting patterns in semi-natural populations of Mus. Nature (Lond.) 354:453.Google Scholar
- Schmutz, S. M., Berryere, T. G., Robbins, J.W., and Carruthers, T. D. (1992). Resistance to Staphylococcus aureusmastitis detected by DNA markers. In Proc. 31st Annu. Mtg. Natl. Mastitis Counc., Arlington, VA., Natl. Mastitis Counc., Inc., Madison, WI, pp. 124–133.Google Scholar
- Schneider, S., Roessli, D., and Excoffier, L. (2000). Arlequin: a Software for Population Genetic Data Analysis, Ver. 2.000, University of Geneva, Geneva.Google Scholar
- Sharif, S., Mallard, B. A., Wilkie, B. N., Sardeant, J. M., Scott, H. M., Dekkers, J. C. M., and Leslie, K. E. (1998). Associations of the bovine major histocompatibility complex DRB3 (BoLA-DRB3) alleles with occurrence of disease and milk somatic cell score in Canadian dairy cattle. Anim. Genet. 29:185–193.PubMedGoogle Scholar
- Weir, B. C., and Cockerham, C. C. (1984). Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370. Zanotti, M., Poli, G., Ponti,W., Polli, M., Rocchi, M., Bolzani, E., Longeri, M., Russo, S., Lewin, H. A., and van Eijk, M. J. T. (1996). Association of BoLA class II haplotypes with subclinical progression of bovine leukaemia virus infection in Holstein-Friesian cattle. Anim. Genet. 27:337-341.Google Scholar