Advertisement

Biochemical Genetics

, Volume 42, Issue 1–2, pp 1–9 | Cite as

Genetic Diversity and Relationship of Yunnan Native Cattle Breeds and Introduced Beef Cattle Breeds

  • Ying Yu
  • Lin-Sheng Lian
  • Ji-Kun Wen
  • Xian-Wei Shi
  • Fang-Xian Zhu
  • Long Nie
  • Ya-Ping Zhang
Article

Abstract

In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic diversity and relationship in 134 samples belonging to two native cattle breeds from the Yunnan province of China (DeHong cattle and DiQing cattle) and four introduced beef cattle breeds (Brahman, Simmental, MurryGrey, and ShortHorn). Ten primers were used, and a total of 84 bands were scored, of which 63 bands (75.0%) were polymorphic. The genetic distance matrix was obtained by proportions of shared fragment. The results indicate that the Yunnnan DeHong cattle breed is closely related to the Brahman (Bos indicus), and the Yunnan DiQing cattle breed is closely related to the Simmental, ShortHorn, and MurryGrey (Bos taurus) breeds. Our results imply that Bos indicus and Bos taurus were the two main origins of Yunnan native cattle. The results also provide the basic genetic materials for conservation of cattle resources and crossbreeding of beef cattle breeds in South China.

native cattle breeds beef cattle breeds RAPD genetic diversity relationship 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

REFERENCES

  1. Bardin, M. G., Bandi, C., Comincini, S., et al. (1992). Use of RAPDs markers to estimate genetic variation in bovine populations. Anim. Genet. 23:57.Google Scholar
  2. Chen, Y. C., Wang, Y. Y., and Cao, H. H., et al. (1990). Characteristics of Chinese Yellow Cattle Ecospecies and Their Course of Utilization, Agricultural Publishing House, Beijing, China.Google Scholar
  3. Gwakisa, P. S., Kemp, S. J., and Teale, A. J. (1994). Characterization of Zebu cattle breeds in Tanzania using random amplified polymorphic DNA markers. Anim. Genet. 25:89-94.Google Scholar
  4. He, Z. Q. (1999). Study on Genetic Diversity of Guizhou Native Cattle Populations Revealed by RAPD, PhD Dissertation, Guizhou Agricultural University.Google Scholar
  5. Kemp, S. J., and Teale, A. J. (1994). Randomly primed PCR amplification of pooled DNA reveals polymorphism in a ruminant repetitive DNA sequence which differentiates Bos indicusand B. taurus . Anim. Genet. 25:83–88.PubMedGoogle Scholar
  6. Kumar, S., Tamura, K., Jakobsen, I. B., and Nei, M. (2001). MEGA2: Molecular Evolutionary Genetics Analysis software, Arizona State University, Tempe, AZ.Google Scholar
  7. Lan, H., Xiong, X. K., and Lin, S. Y., et al. (1993). Mitochondrial DNA polymorphism of cattle and mithum in Yunnan province. Acta Genet. Sin. 20(5):419.PubMedGoogle Scholar
  8. Namikkawa, R., Amano, T., and Kawamoto, Y., et al. (1995). Coat-color variations, blood groups and blood protein/enzyme polymorphisms in the native cattle of Dali Bai and Xishuangbanna Dai autonomous prefectures of Yunnan province and Gayals (Bos gaurus frontalis) in China. Rep. Soc. Res. Native Livestock 15:27–28.Google Scholar
  9. Nei, M. (1979). Mathematical model for studying genetic variation in terms of restriction endonuclease. Proc. Natl. Acad. Sci. U.S.A. 76(10):5267–5272.Google Scholar
  10. Nie, L., Yu, Y., and Zhang, X. Q., et al. (1999). Genetic diversity of cattle in South China as revealed by blood protein electrophoresis. Biochem. Genet. 37:257–265.PubMedGoogle Scholar
  11. Qiu, H., Qing, Z. R., and Chen, Y. C., et al. (1988). Bovine Breeds in China, Shanghai Scientific and Technical Publishers, Shanghai, China.Google Scholar
  12. Wachria, F. N., Waugh, R., and Hackett, C. A., et al. (1995). Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers. Genome 38:201–209.PubMedGoogle Scholar
  13. Williams, J. G. K., Kubelik, A. R., and Livak, K. J., et al. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18:6531–6535.PubMedGoogle Scholar
  14. Yu, Y., Nie, L., and He, Z. Q., et al. (1999). Extensive mitochondrial DNA variation of cattle in South China: Origin and introgression Anim. Genet. 30:1–7.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Ying Yu
    • 1
    • 2
    • 3
  • Lin-Sheng Lian
    • 3
  • Ji-Kun Wen
    • 2
  • Xian-Wei Shi
    • 1
  • Fang-Xian Zhu
    • 2
  • Long Nie
    • 1
  • Ya-Ping Zhang
    • 1
    • 4
  1. 1.Yunnan Laboratory of Molecular Biology of Domestic Animals and Laboratory of Cellular and Molecular Evolution, Kunming Institute of ZoologyChinese Academy of SciencesKunmingP.R. China
  2. 2.Yunnan Beef Cattle and Pasture Research Center, cKunmingP.R. China
  3. 3.College of Animal Science and TechnologyYunnan Agricultural UniversityKunmingP.R. China
  4. 4.Laboratory for Conservation and Utilization of Bio-resourceYunnan UniversityKunmingP.R. China

Personalised recommendations