, Volume 49, Issue 3, pp 315–322 | Cite as

Delivery methods for introducing endophytic bacteria into maize

  • Wellington Bressan
  • Marcela T. Borges


The effectiveness of fivemethods for delivery of ten endophyticbacteria into maize stem and root tissues wasstudied in greenhouse conditions at EmbrapaMilho Sorgo, Sete Lagoas, MG, Brazil. Thedelivery methods included seed inoculation,soil drench, foliar spray, pruned-root dip andseed inoculation + soil drench. The bacterialendophytes were previously isolated from maizeplants, and reinoculated and recovered aftertreatments from maize, cv BR201. Each of thefive methods led to establishment and recoveryof bacterial endophytes in root tissues, butonly four isolates were recovered by the seedtreatment method. All 10 isolates wererecovered by pruned-root dip and seed treatment+ soil drench. No isolates were recovered instem tissues by the seed treatment method, andin the root by foliar spray method. However,all isolates were recovered in stem tissue bypruned-root dip method. The pruned-root dip wasthe most efficient method to deliver bacterialendophytes into maize. The isolate, BR201, wasrecovered by almost all methods in root andstem tissues. The results demonstrate thatendophytic bacteria can be recovered from maizetissues following inoculation by the differentmethods described, but the delivery depends onthe methods used and the endophytic bacterialisolate.

colonization endophytic bacteria root stem 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Embrapa Milho e Sorgo, Microbiology DepartmentSete Lagoas, MGBrazil
  2. 2.Centro Universitário Izabela HendrixBelo Horizonte, MGBrazil

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